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The map label for this gene is ligT [H]

Identifier: 209400335

GI number: 209400335

Start: 165804

End: 166334

Strand: Reverse

Name: ligT [H]

Synonym: ECH74115_0156

Alternate gene names: 209400335

Gene position: 166334-165804 (Counterclockwise)

Preceding gene: 209399556

Following gene: 209400329

Centisome position: 2.99

GC content: 55.74

Gene sequence:

>531_bases
ATGTCTGACCCGCAACGTCTGTTCTTTGCTATCGACTTACCTGCAGAAATCCGCGAACAGATTATCCACTGGCGCGCCAC
ACACTTCCCACTTGAGGCGGGACGTCCGGTCGCCGCCGATAATTTGCATCTGACTCTGGCATTTTTAGGCGAAGTGAGCG
CAGAGAAAGAGAAGGCGCTTTCTCTTTTAGCCGGACGGATTCGTCAACCTGGTTTCACACTCACGCTTGATGACGCCGGC
CAATGGCTGCGTTCGCGTGTGGTGTGGTTAGGGATGCGTCAGCCGCCACGCGGCTTAATCCAGCTGGCGAATATGCTCCG
TTCACAGGCTGCCCGCAGCGGTTGTTTTCAAAGCAATCGTCCGTTTCATCCACATATTACCTTATTGCGCGACGCCAGCG
AGGCGGTGACAATCCCGCCGCCAGGTTTTAACTGGTCATATGCGGTGACGGAGTTCACCCTTTACGCCTCCTCGTTTGCC
CGTGGACGCACACGCTACACGCCGCTAAAACGCTGGGTGCTAACGCAATAA

Upstream 100 bases:

>100_bases
CACGGCAGCAACGGGCAACGACGACACAAAACGCTCCAGAGGGTTAACATTCTTCGCGCCACATTGTAGCATCGCGGTAA
TTCATAACCGAGTGCCTCAC

Downstream 100 bases:

>100_bases
CAAGGATTGTCGCAATGGAATTTTCTCCCCCTCTACAGCGCGCGACGCTAATTCAGCGTTACAAACGTTTTTTAGCCGAT
GTGATCACACCCGATGGTCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
RGRTRYTPLKRWVLTQ

Sequences:

>Translated_176_residues
MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
RGRTRYTPLKRWVLTQ
>Mature_175_residues
SDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQ
WLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFAR
GRTRYTPLKRWVLTQ

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=98.2954545454545, Blast_Score=353, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19964; Mature: 19833

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWVLTQ
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWVLTQ
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]