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The map label for this gene is yjfC [H]

Identifier: 209400204

GI number: 209400204

Start: 5341955

End: 5343118

Strand: Direct

Name: yjfC [H]

Synonym: ECH74115_5702

Alternate gene names: 209400204

Gene position: 5341955-5343118 (Clockwise)

Preceding gene: 209396445

Following gene: 209399702

Centisome position: 95.87

GC content: 53.01

Gene sequence:

>1164_bases
ATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGA
CAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTG
CGGAACTGCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCG
CCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGACGGATGGATTTTGCCTGGTG
TGGCAATGCGCCGGTGAAGCTGCTGGAGTACAACGCCGATACGCCAACTTCATTGTACGAGTCGGCTTATTTCCAGTGGC
TGTGGCTGGAGGATGCCCGGCGCAGCGGCATTATTCCGCGTGATGCCGATCAGTACAATGCTATTCAGGAACGCCTGATT
TCACGCTTTAGCGAGCTTTACAGTCGGGAACCGTTTTATTTTTGCTGCTGTCAGGACACCGATGAAGACAGGAGTACCGT
GCTGTACTTGCAGGACTGCGCCCAGCAGGCAGGGCAGGAGTCGCGGTTTATCTACATTGAAGATCTCGGTTTGGGCGTCG
GCGGCGTACTGACCGATCTTGATGATAATGTCATCCAGCGTGCATTTAAGCTGTATCCGCTGGAGTGGATGATGCGTGAC
GATAACGGTCCGCTGCTGCGCAAGCGTCGCGAGCAATGGGTGGAGCCGTTATGGAAAAGTATCTTGAGTAATAAAGGGCT
AATGCCGCTGCTTTGGCGCTTCTTCCCTGGTCATCCTAACCTTCTTGCATCCTGGTTTGAGGGTGAAAAACCGCAGATTG
CCGCTGGCGAAAGCTATGTGCGTAAACCGATCTACTCGCGCGAAGGCGGAAATGTCACCATTTTCGACGGCCAGAATAAC
GTCGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGA
TAGCTACACACTCATCGGTAGCTGGATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCA
CCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAA

Upstream 100 bases:

>100_bases
CTTACTCTTCAGGCGGCTTTACCACCAAAAAAGCGTCAACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGT
GGGCATTGGGGAGGCTAATC

Downstream 100 bases:

>100_bases
GAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGAC
ACAGTGCACTGGCAAACTCA

Product: glutathionylspermidine synthase domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNN
VVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG

Sequences:

>Translated_387_residues
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNN
VVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG
>Mature_387_residues
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNN
VVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=387, Percent_Identity=99.4832041343669, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=387, Percent_Identity=50.9043927648579, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1789361, Length=374, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 45034; Mature: 45034

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCEEEEEECCC
TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT
CCHHHHHHHHEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
CCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEC
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEECCHHHH
RKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
HCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCH
ACGMGIREDNTLITKDTSRFVPHYIAG
HHCCCCCCCCEEEECCHHHHCCHHHCC
>Mature Secondary Structure
MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCEEEEEECCC
TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT
CCHHHHHHHHEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
CCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEC
DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEECCHHHH
RKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
HCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCH
ACGMGIREDNTLITKDTSRFVPHYIAG
HHCCCCCCCCEEEECCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]