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The map label for this gene is tadA

Identifier: 209400171

GI number: 209400171

Start: 3521854

End: 3522357

Strand: Reverse

Name: tadA

Synonym: ECH74115_3793

Alternate gene names: 209400171

Gene position: 3522357-3521854 (Counterclockwise)

Preceding gene: 209397529

Following gene: 209398316

Centisome position: 63.21

GC content: 53.37

Gene sequence:

>504_bases
TTGTCTGAAGTCGAATTTAGCCACGAATACTGGATGCGTCATGCAATGACGCTGGCGAAGCGTGCCTGGGATGAGCGGGA
AGTACCGGTCGGCGCGGTATTAGTGCATAACAATCGTGTTATTGGCGAAGGCTGGAACCGTCCGATTGGTCGACATGATC
CCACCGCACATGCAGAAATCATGGCCCTGAGGCAGGGCGGTCTGGTGATGCAAAATTATCGTCTGATCGACGCCACGTTG
TATGTCACGCTTGAACCATGTGTAATGTGTGCCGGAGCGATGATCCACAGTCGCATTGGTCGCGTGGTCTTTGGTGCGCG
TGACGCGAAAACTGGCGCTGCGGGATCTTTAATGGATGTGCTGCATCATCCGGGTATGAATCACCGAGTGGAAATTACGG
AAGGAATACTGGCGGATGAGTGCGCGGCGTTGCTCAGTGACTTCTTTCGCATGCGCCGCCAGGAAATTAAAGCGCAGAAA
AAAGCGCAATCCTCGACGGATTAA

Upstream 100 bases:

>100_bases
CTGGCGAGTAACCCCGCGCGGTGAACTCCAGCAACTGGAATAGAGTAAAGCATAGCGTCCGTGTATAATGCGCCGCGCTT
TTATAACCGGAGTTTTCTTT

Downstream 100 bases:

>100_bases
TTTTGTTTCTCTTCACTCCCTTTCCTGGAAAACAGCAGAGAGGGAGAATGGGTCAAATAGTTTGATGACGACTGGGAAAG
AAACGAGAGAAAAGGAAATT

Product: tRNA-specific adenosine deaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATL
YVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
KAQSSTD

Sequences:

>Translated_167_residues
MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATL
YVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
KAQSSTD
>Mature_166_residues
SEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLY
VTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKK
AQSSTD

Specific function: Deaminates adenosine-34 to inosine in tRNA-Arg2

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family

Homologues:

Organism=Homo sapiens, GI124107608, Length=151, Percent_Identity=34.4370860927152, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI145693172, Length=167, Percent_Identity=99.4011976047904, Blast_Score=347, Evalue=2e-97,
Organism=Escherichia coli, GI1786616, Length=168, Percent_Identity=32.1428571428571, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17541138, Length=155, Percent_Identity=38.7096774193548, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554608, Length=97, Percent_Identity=38.1443298969072, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322425, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355527, Length=160, Percent_Identity=33.125, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TADA_ECO57 (Q8XA44)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91057
- PIR:   E85901
- RefSeq:   NP_289115.1
- RefSeq:   NP_311452.1
- ProteinModelPortal:   Q8XA44
- SMR:   Q8XA44
- EnsemblBacteria:   EBESCT00000028202
- EnsemblBacteria:   EBESCT00000060480
- GeneID:   914909
- GeneID:   958078
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3839
- KEGG:   ecs:ECs3425
- GeneTree:   EBGT00050000008984
- HOGENOM:   HBG741046
- OMA:   DACSAQL
- ProtClustDB:   PRK10860
- BioCyc:   ECOL83334:ECS3425-MONOMER
- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193

Pfam domain/function: PF00383 dCMP_cyt_deam_1; SSF53927 Cytidine_deaminase-like

EC number: NA

Molecular weight: Translated: 18736; Mature: 18605

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHH
MALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDV
HHHHCCCEEEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQSSTD
HHCCCCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHH
MALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDV
HHHHCCCEEEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQSSTD
HHCCCCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796