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The map label for this gene is artJ [H]

Identifier: 209400027

GI number: 209400027

Start: 1031804

End: 1032535

Strand: Reverse

Name: artJ [H]

Synonym: ECH74115_1019

Alternate gene names: 209400027

Gene position: 1032535-1031804 (Counterclockwise)

Preceding gene: 209396948

Following gene: 209398497

Centisome position: 18.53

GC content: 46.86

Gene sequence:

>732_bases
ATGAAAAAGTTAGTTCTTGCCGCATTACTGGCATCCTGTGCGTTCGGCGCTTCTGCCGCAGAGAAAATCAATTTTGGCGT
ATCAGCGACCTATCCGCCGTTCGAGTCGATGGATGCCAATAACCAGATCGTCGGCTTTGATATCGACCTAGCAAAAGCGT
TATGCAAACAGATGCAGGCAGAATGTACTTTTACCAATCACGCGTTCGACAGCCTGATCCCGTCACTGAAATTCAGGAAA
TATGACGCCGTAATCTCCGGTATGGATATCACACCAGATCGTAGCAAGCAGGTATCGTTCACCACGCCCTATTACGAAAA
CTCAGCCGTCGTGATTGCCAAAAAAGATACCTACAAAACGTTTGCCGACCTGAAAGGCAAACGCATTGGGATGGAAAACG
GTACTACGCACCAGAAATATATTCAGGATCAGCACCCGGAAGTGAAAACGGTCTCCTATGACAGTTATCAAAATGCCTTT
ATCGATCTGAAAAATAGCCGTATTGATGGCGTATTTGGTGACACTGCGGTGGTTAATGAATGGCTGAAAACCAATCCGCA
ACTGGGCGTTGCTACTGAAAAAGTGACCGATCCGCAATACTTTGGCACCGGTCTGGGTATCGCTGTACGTCCGGATAACA
AAGCTCTGCTGGAAAAACTGAATAACGCGCTGGCAGCAATTAAAGCTGACGGTACATATCAACAAATCAGCGATCAGTGG
TTCCCGCAATAA

Upstream 100 bases:

>100_bases
TTTAATCATATTTATTGCATATAAATTCACTTGATGGCATTGTTATCCCATGCCGCAGACACGGCCAATAATCATAAGAT
TGACAGACGGGAGTTCCATC

Downstream 100 bases:

>100_bases
TTATTCATTTGCCGGAGTGGGCGAACACCCACTCCGGCAAAATCACTTCTCTACGCTAATCCCAGGCGCTGAAACTGCTC
TGGCGGCGCATCATTATCTT

Product: arginine ABC transporter, periplasmic arginine-binding protein ArtJ

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPDRSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQQISDQW
FPQ

Sequences:

>Translated_243_residues
MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPDRSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQQISDQW
FPQ
>Mature_243_residues
MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIVGFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRK
YDAVISGMDITPDRSKQVSFTTPYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQQISDQW
FPQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=96.7078189300412, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=63.9004149377593, Blast_Score=339, Evalue=9e-95,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=38.8646288209607, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=35.5731225296443, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=31.8181818181818, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=29.0983606557377, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26847; Mature: 26847

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIVGFDIDLAKALCKQMQA
CHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
ECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTTPYYENSAVVIAKKDTYKT
CCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAFIDLKNSRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCCCCCHHHHHH
WLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQQISDQW
HHHCCCCCCCEECCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHEEEECCCCHHHHHHHC
FPQ
CCC
>Mature Secondary Structure
MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIVGFDIDLAKALCKQMQA
CHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
ECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTTPYYENSAVVIAKKDTYKT
CCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHH
FADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAFIDLKNSRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCEECCCCCCHHHHHH
WLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLEKLNNALAAIKADGTYQQISDQW
HHHCCCCCCCEECCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHEEEECCCCHHHHHHHC
FPQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]