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The map label for this gene is wcaD [H]

Identifier: 209399912

GI number: 209399912

Start: 2769871

End: 2771088

Strand: Reverse

Name: wcaD [H]

Synonym: ECH74115_2991

Alternate gene names: 209399912

Gene position: 2771088-2769871 (Counterclockwise)

Preceding gene: 209398209

Following gene: 209396416

Centisome position: 49.73

GC content: 42.53

Gene sequence:

>1218_bases
ATGTCAACTTCTATCAGAATCTGTAGCTACCTGCTGCTGCCGCTGATCTACCTGCTGGTTAACGTCAAAATCACCCAGCT
TGGCGAAAGTTTCCCCATCACCATCGTCACTTTTTTACCTGTCTTGTTACTGCTGTTTTTAGAACGCATCAGCGTTAAAA
AATTGATGATTGCCTTAGGGATTTGCGCGGGACTCACGGCGTTTAACTACCTGTTTGGTCAGTCGCTGGATGCCAGTAAA
TACGTCACTTCAACTATGCTGTTTGTCTATATCGTGATCATTATTGGTATGGTGTGGAGTATTCGCTTTAAAACGATTTC
GCCACACAACCATCGCAAGATATTACGTTTCTTTTATCTGGTGGTCGGGCTGGTGGTGGCGCTGGCGGCGGTGGAGATGG
CGCAAATTATCCTCACCGGCGGCAGCAGTATTATGGAGTCGATTTCGAAATATCTGATTTACAGCAACAGCTATGTGCTG
AATTTCATCAAATTCGGCGGCAAGCGCACGACAGCACTTTATTTCGAACCGGCATTTTTCGCTCTGGCATTAATCTCAAT
TTGGCTCAGCATCAAACAGTTTGGTATCAAAACGCCTAAAACAGATGCTATGATTCTCGCAGGGATAATATTATCCGGAT
CGTTTTCAGGGGTTATGACCTTTATCCTGTTTTATTTGCTGGAGTGGGCATTTCAATATCTGAATAAAGAGGCGATTAAG
AAAAAGTTACCGTTAGCATTGATTTCTCTGGCAGTATTCCTGGTTGGTGTGGTAATTGCATTTCCTTATATTTCCACCCG
TCTGGGCGATTTAGGTACGGAAGGATCGTCATCGTATTATCGTATTGTCGGTCCGCTGGTGATGGTCGGTTATTCTTTGA
CCCATATTGACGGTGTAGTCAGATTTGGCTCACTTTATGAATATGTCGCATCATTCGGAATATTTAACGGTGCGGATGTC
GGAAAAACCATAGACAATGGTTTGTATCTGCTGATTATTTATTTTTCTTGGTTCGCGGTGTTTTTATCGCTGTGGTACAT
GGGGAAAGTGATAAAAATGATGATCAACGCTTTTGGTGATAACCGCAATTTTCGCGTGCAATTATATCTTTTTACTCCGG
TATCGCTGTTTTTTACCGGTTCGATATTTAGCCCGGAATATGCATTTTTAATCGTCTGTCCGTTTATTTTGCGAAAAGCG
TTAAATATTACGAGGTAA

Upstream 100 bases:

>100_bases
AGCAAACCGGAAATCGCGCAGGCGTTATTTGGTACCACGCTGGCTGAGTTCAGCCAACGCAGCCGCGCCGCCTACAGTGG
ACAACAGATGCTGGAGGAGT

Downstream 100 bases:

>100_bases
GAATAAGAACATGTTGCTTAGCATAATCACTGTCGCGTTTCGTAACCTCGAAGGGATAGTCAAAACACATGCCTCGCTGG
CGCATCTGGCGCAGGCGGAA

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLLLFLERISVKKLMIALGICAGLTAFNYLFGQSLDASK
YVTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKEAIK
KKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADV
GKTIDNGLYLLIIYFSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKA
LNITR

Sequences:

>Translated_405_residues
MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLLLFLERISVKKLMIALGICAGLTAFNYLFGQSLDASK
YVTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKEAIK
KKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADV
GKTIDNGLYLLIIYFSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKA
LNITR
>Mature_404_residues
STSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLLLFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKY
VTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVLN
FIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKEAIKK
KLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVG
KTIDNGLYLLIIYFSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKAL
NITR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788369, Length=405, Percent_Identity=99.5061728395062, Blast_Score=795, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45486; Mature: 45355

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLLLFLERISVKKLMIALG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRFFYL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
VVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVLNFIKFGGKRTTALYFEPAFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECHHHH
ALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKEAIK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
KKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVV
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIYFSWFAVFLSLWYMGKVIKMMINAFGD
HHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKALNITR
CCCEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLLLFLERISVKKLMIALG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIRFKTISPHNHRKILRFFYL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
VVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVLNFIKFGGKRTTALYFEPAFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECHHHH
ALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGVMTFILFYLLEWAFQYLNKEAIK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
KKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGSSSYYRIVGPLVMVGYSLTHIDGVV
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIYFSWFAVFLSLWYMGKVIKMMINAFGD
HHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIVCPFILRKALNITR
CCCEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]