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The map label for this gene is chpB [H]

Identifier: 209399815

GI number: 209399815

Start: 5379355

End: 5379705

Strand: Direct

Name: chpB [H]

Synonym: ECH74115_5742

Alternate gene names: 209399815

Gene position: 5379355-5379705 (Clockwise)

Preceding gene: 209400249

Following gene: 209400962

Centisome position: 96.54

GC content: 53.56

Gene sequence:

>351_bases
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Upstream 100 bases:

>100_bases
GCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCC
ACCCCTGCGGGTGACGAAAT

Downstream 100 bases:

>100_bases
GGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACT
TATTTATTCTTTGCGCGCTC

Product: toxin ChpB

Products: NA

Alternate protein names: Endoribonuclease ChpB; Toxin ChpB [H]

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Sequences:

>Translated_116_residues
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE
>Mature_116_residues
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Specific function: Part of a toxin-antitoxin (TA) module. ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. Cleavage is independent of the ribosome. Cleavage occurs at ACY sequences where Y is no

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1790672, Length=116, Percent_Identity=99.1379310344828, Blast_Score=231, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 12512; Mature: 12512

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARY
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEEECCCCCEEEE
AGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE
CCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARY
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEEECCCCCEEEE
AGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE
CCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226627; 7610040; 9278503; 3525538; 2848015; 8083180 [H]