Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 209399667

GI number: 209399667

Start: 4022681

End: 4024573

Strand: Reverse

Name: parE [H]

Synonym: ECH74115_4344

Alternate gene names: 209399667

Gene position: 4024573-4022681 (Counterclockwise)

Preceding gene: 209400424

Following gene: 209395843

Centisome position: 72.23

GC content: 53.94

Gene sequence:

>1893_bases
ATGACGCAAACTTATAACGCTGATGCCATTGAGGTACTCACCGGGCTTGAGCCGGTTCGCCGCCGTCCGGGGATGTATAC
CGATACCACTCGCCCTAACCATTTGGGGCAAGAAGTCATTGATAACAGTGTGGATGAAGCACTGGCGGGTCACGCAAAAC
GCGTGGACGTTATTTTACATGCTGACCAGTCGTTAGAAGTTATTGACGATGGGCGCGGGATGCCGGTGGATATTCACCCG
GAAGAGGGTGTACCGGCGGTTGAACTGATTCTTTGCCGTCTGCATGCAGGCGGTAAATTCTCTAACAAAAATTACCAGTT
CTCTGGCGGCCTGCATGGCGTGGGGATTTCGGTGGTTAACGCCCTGTCGAAGCGCGTAGAAGTTAACGTGCGCCGCGATG
GTCAGGTTTATAACATCGCCTTTGAAAATGGCGAAAAGGTGCAGGATTTACAGGTTGTCGGCAATTGCGGTAAACGCAAT
ACCGGCACCAGCGTCCATTTCTGGCCGGATGAAACCTTCTTCGATAGCCCGCGTTTTTCTGTTTCACGCCTGACGCATGT
GCTGAAAGCCAAAGCAGTACTGTGCCCCGGCGTTGAGATTACCTTTAAAGATGAGATCAACAACACCGAACAGCGCTGGT
GCTATCAGGACGGTCTGAATGATTACCTGGCGGAAGCGGTAAACGGTTTACCGACGCTGCCAGAAAAACCGTTTATCGGT
AATTTCGCTGGCGATACTGAAGCGGTGGACTGGGCGCTACTGTGGCTGCCGGAAGGCGGTGAACTGCTGACCGAAAGCTA
CGTCAACCTGATCCCAACGATGCAGGGCGGTACCCATGTTAATGGCCTGCGTCAGGGCCTGCTGGACGCGATGCGTGAGT
TCTGTGAATACCGCAACATTTTGCCGCGCGGTGTAAAGTTGTCGGCGGAAGATATCTGGGATCGCTGCGCCTATGTGCTG
TCAGTAAAAATGCAGGATCCACAGTTTGCCGGGCAGACCAAAGAGCGTCTCTCTTCGCGTCAGTGTGCGGCATTCGTTTC
GGGCGTGGTGAAAGATGCCTTCATCCTGTGGCTGAACCAGAACGTTCAGGCGGCGGAGTTGCTGGCAGAGATGGCGATTT
CCAGCGCCCAGCGTCGTATGCGTGCGGCTAAAAAAGTGGTGCGTAAAAAGCTGACCAGCGGCCCGGCGCTGCCTGGCAAA
CTGGCTGATTGTACCGCGCAGGACCTTAACCGTACCGAACTGTTCCTCGTGGAAGGTGACTCCGCAGGCGGATCTGCCAA
GCAGGCGCGCGATCGCGAATATCAGGCGATCATGCCGCTGAAAGGTAAGATCCTTAACACCTGGGAAGTTTCTTCCGACG
AAGTGCTGGCCTCGCAGGAAGTGCATGATATTTCGGTAGCGATCGGTATCGATCCTGACAGCGACGATCTGAGCCAGCTT
CGTTATGGCAAAATCTGTATCCTCGCGGATGCGGACTCTGATGGTTTGCACATTGCTACACTGCTCTGCGCTTTGTTCGT
AAAACACTTCCGCGCGTTGGTGAAACACGGTCACGTTTACGTCGCACTGCCACCGCTCTACCGTATTGATCTCGGGAAAG
AGGTTTATTACGCGCTGACGGAAGAAGAGAAAGAGGGCGTACTTGAGCAATTAAAACGCAAGAAAGGCAAGCCGAACGTC
CAGCGTTTTAAAGGTCTGGGGGAAATGAACCCGATGCAATTGCGCGAAACCACGCTTGATCCGAACACTCGCCGTCTGGT
GCAGCTGACTATCGATGATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAG
ATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTCTGA

Upstream 100 bases:

>100_bases
AACCACGCATTCACGGGCTTCGAAGATTATTTCAACCCGATCGTCGATTTTCTTGGTCTGCACCATCTCTGACGATCAAC
TCGAATTGCTAACTTAAACC

Downstream 100 bases:

>100_bases
TAATCCTCCCGCCAGAAGAATAAAACATAGCCCGGTATACAAGTGTATACCGGGCTTTTTTGATCAAAATTCCATTTTCA
CCGTGAATTGCACTTCACGC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Sequences:

>Translated_630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
>Mature_629_residues
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPE
EGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNT
GTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGN
FAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLS
VKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKL
ADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLR
YGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQ
RFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=577, Percent_Identity=25.1299826689775, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI19913406, Length=582, Percent_Identity=24.0549828178694, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=99.8412698412698, Blast_Score=1304, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=40.7207207207207, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645845, Length=606, Percent_Identity=24.0924092409241, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=597, Percent_Identity=24.4556113902848, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=230, Percent_Identity=26.9565217391304, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=683, Percent_Identity=23.7188872620791, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17136538, Length=582, Percent_Identity=22.8522336769759, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70258; Mature: 70127

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]