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The map label for this gene is pinE [H]

Identifier: 209399537

GI number: 209399537

Start: 2814234

End: 2814827

Strand: Reverse

Name: pinE [H]

Synonym: ECH74115_3031

Alternate gene names: 209399537

Gene position: 2814827-2814234 (Counterclockwise)

Preceding gene: 209398160

Following gene: 209397008

Centisome position: 50.52

GC content: 52.86

Gene sequence:

>594_bases
ATGCTTATTGGCTATGTACGCGTGTCAACAAATGACCAGAACACGGAATTGCAGCGTAATGCGCTGGAGTGTGCAGGATG
TGAGCTGATTTTTGAGGATAAAATCAGCGGCACGAAGTCCGACCGACCGGGACTGAAAAAACTGCTCAGAACATTATCAG
CAGGTGACACTCTGGTAGTCTGGAAGCTGGATAGGCTGGGGCGTAGTATGCGGCATCTGGTCATTCTGGTTGAGGAACTG
CGCGAACGCGGCGTTAATTTTCGCAGCCTGACGGATGCTATTGATACCAGCACGCCGATGGGGCGCTTTTTCTTTCATGT
GATGGGGGCCCTGGCAGAAATGGAGCGAGAACTCATTGTCGAGCGGACACGCGCCGGACTGGAAGCGGCCAGAGCCAAAG
GTCGTATTGGTGGCAGACGACCGAAACTCACCACGAGTGAGTGGGAGCAGGTCGGACGGTTGCTGGCTGCGGGGGAATCT
CGTCAACGTGTGGCGCTGATTTTTGATATTGGCCTGTCCACGCTCTATAAAAAATTCCCCTCATCAGCGACAAAGAATAA
ATTGTGTCATCCCTTAGCCAACCGGGACAAATAG

Upstream 100 bases:

>100_bases
CAACGGGTAATGCCGAACCTTCTCCCAAACCAACGTTTATGAAAATGCAGAAATAACGGGTAACTGGCATCATCTCCGGT
TTTTATTCAGGGAGATGCTC

Downstream 100 bases:

>100_bases
CCTGACATCTCCGGCACAACTGAAAATATCACTCACCCATTAACCACGGAGTTAAACGGATGAGTGATTATCATCACGGC
GTGCAGGTGCTGGAGATTAA

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK

Sequences:

>Translated_197_residues
MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK
>Mature_197_residues
MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=86.7403314917127, Blast_Score=333, Evalue=4e-93,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=37.7049180327869, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=37.7049180327869, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22143; Mature: 22143

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGESRQRVALIFDIGLSTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC
SSATKNKLCHPLANRDK
CCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGESRQRVALIFDIGLSTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC
SSATKNKLCHPLANRDK
CCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]