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The map label for this gene is caiA [H]

Identifier: 209399530

GI number: 209399530

Start: 43651

End: 44793

Strand: Reverse

Name: caiA [H]

Synonym: ECH74115_0043

Alternate gene names: 209399530

Gene position: 44793-43651 (Counterclockwise)

Preceding gene: 209399200

Following gene: 209397439

Centisome position: 0.8

GC content: 53.81

Gene sequence:

>1143_bases
ATGGATTTTAATTTAAATGATGAGCAGGAACTGTTTGTCGCCGGTATCCGCGAACTGATGGCCAGCGAAAACTGGGAGGC
CTATTTTGCCGAGTGCGACCGTGACAGCGTCTACCCGGAACGTTTTGTCAAAGCACTGGCGGATATGGGTATCGACAGTC
TGCTGATCCCTGAAGAGCACGGTGGTCTGGACGCGGGGTTTGTTACTCTCGCCGCCGTGTGGATGGAGCTGGGACGTCTG
GGGGCACCAACCTATGTACTGTACCAGTTGCCGGGCGGGTTCAATACCTTCCTGCGCGAAGGCACACAAGAGCAGATCGA
CAAGATTATGGCTTTCCGCGGCACCGGTAAGCAGATGTGGAACTCAGCGATTACCGAACCGGGCGCGGGCTCCGACGTGG
GTAGCCTGAAAACGACTTATACCCGTAGAAATGGTAAGATTTATCTTAATGGTAGTAAGTGTTTTATTACCAGTAGCGCC
TACACCCCGTACATCGTGGTGATGGCGCGCGACGGGGCTTCTCCGGACAAACCTGTCTACACCGAATGGTTTGTTGATAT
GAGCAAACCGGGCATCAAAGTGACCAAACTTGAGAAGCTCGGTCTGCGTATGGATAGCTGCTGTGAAATCACCTTTGACG
ATGTGGAACTGGACGAGAAAGACATGTTCGGTCGGGAAGGTAACGGCTTTAACCGCGTCAAAGAAGAGTTCGACCATGAA
CGTTTCCTGGTAGCCCTGACCAACTACGGTACGGCGATGTGCGCCTTTGAAGATGCGGCGCGCTACGCCAACCAGCGCGT
GCAGTTTGGCGAGGCTATTGGTCGTTTCCAGTTGATTCAGGAAAAATTCGCCCACATGGCGATCAAATTAAACTCCATGA
AAAACATGCTGTATGAAGCAGCGTGGAAAGCAGACAACGGCACCATCACCTCTGGCGATGCAGCGATGTGCAAATACTTC
TGCGCCAATGCGGCATTTGAAGTGGTGGATAGCGCAATGCAGGTGCTGGGCGGTGTCGGGATTGCGGGCAACCACCGCAT
CAGCCGCTTCTGGCGTGACCTGCGTGTAGACCGCGTTTCCGGAGGATCTGACGAAATGCAGATCCTGACGCTGGGTCGTG
CGGTGCTGAAGCAATACCGCTAA

Upstream 100 bases:

>100_bases
GCTGTTCTTCGTCAACATTATGGTGACGCTCTCCTTTATTAAAGACGCGAAACAGAACTGGAAAGATTAATTAACCCCCA
AAATATCAAGAGGTTGAAAG

Downstream 100 bases:

>100_bases
GTCCGTGCGCTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGAGCACGAATGTAGGCCGGATAAGGCGTTTA
CGCCGCATCCGGCAGTCATG

Product: crotonobetainyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Sequences:

>Translated_380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR
>Mature_380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=354, Percent_Identity=29.9435028248588, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4501859, Length=379, Percent_Identity=26.9129287598945, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI226958412, Length=369, Percent_Identity=28.4552845528455, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI226958414, Length=340, Percent_Identity=28.8235294117647, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4557231, Length=389, Percent_Identity=28.0205655526992, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI187960098, Length=389, Percent_Identity=28.0205655526992, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI21361497, Length=363, Percent_Identity=31.129476584022, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4501857, Length=378, Percent_Identity=24.8677248677249, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI76496475, Length=381, Percent_Identity=29.6587926509186, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4557235, Length=381, Percent_Identity=29.6587926509186, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI7656849, Length=386, Percent_Identity=26.6839378238342, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4503943, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI7669494, Length=262, Percent_Identity=25.9541984732824, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI48976061, Length=296, Percent_Identity=23.3108108108108, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI211971008, Length=296, Percent_Identity=23.3108108108108, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI38505218, Length=345, Percent_Identity=23.1884057971014, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786223, Length=380, Percent_Identity=100, Blast_Score=792, Evalue=0.0,
Organism=Escherichia coli, GI87081958, Length=382, Percent_Identity=47.9057591623037, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI87082384, Length=329, Percent_Identity=25.2279635258359, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=382, Percent_Identity=31.6753926701571, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17569725, Length=368, Percent_Identity=28.5326086956522, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17534899, Length=393, Percent_Identity=28.498727735369, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17508101, Length=380, Percent_Identity=25.7894736842105, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17506239, Length=382, Percent_Identity=27.4869109947644, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17570075, Length=389, Percent_Identity=28.5347043701799, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17533517, Length=356, Percent_Identity=27.247191011236, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71985184, Length=380, Percent_Identity=26.8421052631579, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505929, Length=361, Percent_Identity=25.4847645429363, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17551932, Length=369, Percent_Identity=24.9322493224932, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71990804, Length=296, Percent_Identity=27.3648648648649, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985192, Length=320, Percent_Identity=25.9375, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17534353, Length=278, Percent_Identity=28.4172661870504, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982178, Length=278, Percent_Identity=22.6618705035971, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562446, Length=334, Percent_Identity=23.3532934131737, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17531909, Length=248, Percent_Identity=20.9677419354839, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21356377, Length=378, Percent_Identity=28.5714285714286, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24646207, Length=351, Percent_Identity=29.6296296296296, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355753, Length=344, Percent_Identity=31.1046511627907, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24666513, Length=378, Percent_Identity=27.5132275132275, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI281363737, Length=352, Percent_Identity=30.1136363636364, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24660351, Length=350, Percent_Identity=28.8571428571429, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI19920834, Length=282, Percent_Identity=26.241134751773, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42559; Mature: 42559

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEH
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMW
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSAYTPYIVVMARDGASPDKPVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCCC
TEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
HHHEEECCCCCEEEEEHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHCCCC
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEA
EEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVS
HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEH
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMW
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSAYTPYIVVMARDGASPDKPVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCCC
TEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
HHHEEECCCCCEEEEEHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHCCCC
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEA
EEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVS
HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA