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The map label for this gene is cmtB

Identifier: 209399343

GI number: 209399343

Start: 3923895

End: 3924338

Strand: Reverse

Name: cmtB

Synonym: ECH74115_4235

Alternate gene names: 209399343

Gene position: 3924338-3923895 (Counterclockwise)

Preceding gene: 209400255

Following gene: 209398789

Centisome position: 70.43

GC content: 42.57

Gene sequence:

>444_bases
ATGCGGCTTAGTGACTATTTTCCAGAATCATCAATCTCAGTTATACATTCAGCAAAAGATTGGCAGGAAGCTATCGATTT
CTCGATGGTATCATTGCTGGATAAAAACTATATCAGCGAGAATTACATTCAGGCAATTAAAGATTCCACCATTAACAATG
GCCCTTATTATATTCTCGCACCAGGCGTGGCAATGCCTCATGCGCGACCGGAATGTGGGGCGCTTAAAACCGGGATGTCT
TTGACATTACTTGAACAAGGTGTTTATTTTCCGGGGAATGACGAACCAATTAAATTACTCATCGGACTCTCTGCTGCCGA
TGCCGATTCGCACATTGGCGCTATTCAGGCGTTAAGTGAATTACTGTGCGAAGAAGAAATACTCGAACAACTCTTAACAG
CATCATCAGAAAAACAATTAGCGGACATTATCAGCCGCGGATAA

Upstream 100 bases:

>100_bases
TCAAAACACCGGTTTCCCTGCTCAATTGCTTTCATTAAACCGCACGAGTATTTTATTTTTACCGCCGGACGGTACCCTAA
AAATAAAATAAGGATAATTT

Downstream 100 bases:

>100_bases
TTACTCTCTCCTCTACAGGATAATATTATGGAAAACAAGTCTGCTCGTGCAAAGGTCCAGGCTTTTGGGGGCTTTTTGAC
TGCAATGGTCATCCCCAATA

Product: putative mannitol phosphotransferase subunit EIIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS
LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG

Sequences:

>Translated_147_residues
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS
LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG
>Mature_147_residues
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS
LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1789302, Length=147, Percent_Identity=100, Blast_Score=300, Evalue=2e-83,
Organism=Escherichia coli, GI2367359, Length=150, Percent_Identity=37.3333333333333, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1790755, Length=132, Percent_Identity=37.8787878787879, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTMA_ECO57 (P69825)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98105
- PIR:   D85950
- RefSeq:   NP_289505.1
- RefSeq:   NP_311836.1
- ProteinModelPortal:   P69825
- SMR:   P69825
- EnsemblBacteria:   EBESCT00000024416
- EnsemblBacteria:   EBESCT00000059898
- GeneID:   916372
- GeneID:   959538
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4278
- KEGG:   ecs:ECs3809
- GeneTree:   EBGT00050000009676
- HOGENOM:   HBG697201
- OMA:   YIQAIKG
- ProtClustDB:   PRK09854
- BioCyc:   ECOL83334:ECS3809-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- Gene3D:   G3DSA:3.40.930.10

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 16046; Mature: 16046

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
CCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLCEEEILEQLLTASSEKQLADIISRG
HHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
CCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLCEEEILEQLLTASSEKQLADIISRG
HHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796