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The map label for this gene is nlpA

Identifier: 209399316

GI number: 209399316

Start: 4728613

End: 4729431

Strand: Reverse

Name: nlpA

Synonym: ECH74115_5086

Alternate gene names: 209399316

Gene position: 4729431-4728613 (Counterclockwise)

Preceding gene: 209399558

Following gene: 209398567

Centisome position: 84.88

GC content: 46.76

Gene sequence:

>819_bases
ATGAAACTGACAACACATCATCTACGGGCGGGGGCCGCATTATTACTGGCAGGAGTTCTGCTGGCAGGTTGCGACCAGAG
CAGCAGCGATGAAAAGCATATTAAAGTTGGCGTTATTAATGGCGCAGAACAAGATGTCGCAGAAGTCGCTAAAAAGGTAG
CAAAAGAGAAATATGGCCTCGATGTTGAACTGGTAGGATTTAGCGGTTCGTTGCTACCGAACGATGCGACAAATAATGGC
GAGCTTGATGCGAACGTCTTCCAGCATCGACCCTTTCTTGAACAAGATAATCAGACGCATGGTTATAAACTGGTAGCGGT
GGGAAATACGTTTGTCTTCCCTATGGCGGGCTATTCCAAAAAAATCAAAACCGTGGCGCAAATAAAAGAAGGTGCGACAG
TGGCGATCCCCAACGACCCGACTAACCTTGGTCGCGCGCTTTTACTGCTGCAAAAAGAGAAGCTAATTACTCTGAAAGAG
GGAAAAGGGTTATTACCTACCGCGTTGGATATAACCGATAATCCACGCCATTTGCAGATAATGGAGCTGGAAGGGGCACA
GTTGCCACGTGTACTGGACGACCCGAAAGTGGATGTAGCGATTATCAGCACCACTTACATCGAGCAAACAGGACTTTCTC
CGGTTAACGACAGCGTATTTATTGAAGATAAAAATTCGCCGTATGTGAATATTCTGGTGGCACGGGAAGATAATAAGAAT
GCAGAAAACGTGAAGGAATTTCTGCAATCTTATCAATCACCCGAAGTCGCTAAAGCGGCAGAAACCATCTTTAACGGTGG
TGCGGTGCCTGGCTGGTAA

Upstream 100 bases:

>100_bases
CACTTTTATTCCTTTTTATTCTTATCGATAGCGTTTTGTTTTTTAAACTGCAGCGACCTTACCGCTATAGTCCGGTAATC
ATTAATAAAAGGATAAAAAA

Downstream 100 bases:

>100_bases
TTCTCACTCATGCCGCGCCGGACGATTTATTTTTCTGGCGCGGCGGCGGGAATCCATTGAAATCAAAATCACCGATGACA
GAATCAGCAGCGTTCCCAGC

Product: cytoplasmic membrane lipoprotein-28

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNG
ELDANVFQHRPFLEQDNQTHGYKLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDSVFIEDKNSPYVNILVAREDNKN
AENVKEFLQSYQSPEVAKAAETIFNGGAVPGW

Sequences:

>Translated_272_residues
MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNG
ELDANVFQHRPFLEQDNQTHGYKLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDSVFIEDKNSPYVNILVAREDNKN
AENVKEFLQSYQSPEVAKAAETIFNGGAVPGW
>Mature_272_residues
MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNG
ELDANVFQHRPFLEQDNQTHGYKLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDSVFIEDKNSPYVNILVAREDNKN
AENVKEFLQSYQSPEVAKAAETIFNGGAVPGW

Specific function: Unknown

COG id: COG1464

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface antigen

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpA lipoprotein family

Homologues:

Organism=Escherichia coli, GI1790093, Length=272, Percent_Identity=97.4264705882353, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786396, Length=263, Percent_Identity=61.9771863117871, Blast_Score=348, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NLPA_ECO57 (Q8XC50)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91203
- PIR:   D86049
- RefSeq:   NP_290292.1
- RefSeq:   NP_312622.1
- ProteinModelPortal:   Q8XC50
- SMR:   Q8XC50
- EnsemblBacteria:   EBESCT00000025818
- EnsemblBacteria:   EBESCT00000058260
- GeneID:   915435
- GeneID:   960822
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5147
- KEGG:   ecs:ECs4595
- GeneTree:   EBGT00050000009441
- HOGENOM:   HBG661773
- OMA:   DNKAHNY
- ProtClustDB:   PRK09861
- BioCyc:   ECOL83334:ECS4595-MONOMER
- InterPro:   IPR004872
- InterPro:   IPR004478
- TIGRFAMs:   TIGR00363

Pfam domain/function: PF03180 Lipoprotein_9

EC number: NA

Molecular weight: Translated: 29423; Mature: 29423

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKEKYGL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
DVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGYKLVAVGNTFVFPMAGYSK
CEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCEEEEECCCCHH
KIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKEGKGLLPTALDITDNPRHLQI
HHHHHHHHHCCCEEEECCCCHHHHHEEEEEECCCEEEEECCCCCCCEEEECCCCCCEEEE
MELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDSVFIEDKNSPYVNILVAREDNKN
EEECCCCCCHHCCCCCCEEEEEEEEEHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
AENVKEFLQSYQSPEVAKAAETIFNGGAVPGW
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKEKYGL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
DVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGYKLVAVGNTFVFPMAGYSK
CEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCEEEEECCCCHH
KIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKEGKGLLPTALDITDNPRHLQI
HHHHHHHHHCCCEEEECCCCHHHHHEEEEEECCCEEEEECCCCCCCEEEECCCCCCEEEE
MELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDSVFIEDKNSPYVNILVAREDNKN
EEECCCCCCHHCCCCCCEEEEEEEEEHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
AENVKEFLQSYQSPEVAKAAETIFNGGAVPGW
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796