Click here to switch to the map view.

The map label for this gene is ubiH [H]

Identifier: 209399230

GI number: 209399230

Start: 3893866

End: 3895044

Strand: Reverse

Name: ubiH [H]

Synonym: ECH74115_4200

Alternate gene names: 209399230

Gene position: 3895044-3893866 (Counterclockwise)

Preceding gene: 209398591

Following gene: 209397458

Centisome position: 69.9

GC content: 59.54

Gene sequence:

>1179_bases
ATGAGCGTAATCATCGTCGGTGGCGGCATGGCGGGCGCGACGCTGGCGCTGGCTATTTCCCGGTTAAGTCACGGGGCGCT
GCCGGTACATTTGATTGAAGCGACTGCGCCAGAGTCACATGCTCATCCGGGCTTTGATGGACGAGCGATAGCGCTGGCGG
CGGGTACCTGTCAGCAACTGGCGCGCATCGGCGTTTGGCAATCTCTGGCGCATTGCGCAACTGCCATCACCACCGTGCAT
GTCAGCGATCGAGGTCACGCTGGATTTGTCACCCTCGCCGCAGAAGATTACCAACTGGCGGCGCTGGGACAGGTTGTCGA
ATTGCACAATGTCGGGCAACGGCTGTTTGCATTGCTGCGTAAAGCACCTGGCGTAACGCTGCATTGCCCTGATCGCGTGG
CTAACGTTGCCCGTACTCAGAGTCACGTTGAAGTGACGCTGGAGGGTGGCGAGACGCTGACGGGCCGCGTGCTGGTAGCC
GCTGATGGCACCCATTCAGCGTTAGCCACTGTGTGCGGCGTTGACTGGCAGCAGGAGCCTTACGAACAACTGGCTGTGAT
TGCCAACGTTGCGACTTCCGTTGCGCATGAAGGGCGCGCTTTTGAACGCTTTACGCAACATGGCCCGCTGGCGATGTTGC
CGATGTCTGACGGACGCTGTTCGCTGGTCTGGTGTCATCCACTGGAACGGCGCGAAGAGGTGCTGTCGTGGAGCGACGAG
AAGTTTTGCCGTGAACTCCAGTCGGCCTTTGGCTGGCGACTTGGGAAAATTACCCACGCTGGTAAACGCAGTGCTTATCC
GCTGGCGTTAACCCACGCCGCCAGATCTATTACCCATCGTACCGTGCTGGTGGGCAATGCGGCGCAAACCCTGCACCCGA
TTGCCGGGCAAGGGTTCAACCTCGGTATGCGAGATGTCATGAGTCTTGCGGAAACCCTGACTCAGGCGCAGGAGCGCGGA
GAAGACATGGGTGATTACGGCGTATTGTGTCGTTATCAGCAGCGTCGACAGAGCGATCGCGAAGCAACCATTGGCGTCAC
GGACAGCCTTGTACATCTTTTTGCCAACCGTTGGACGCCACTGGTTGTCGGGCGCAACATCGGGCTGATGACGATGGAAT
TATTCACCCCGGCACGCGATGTGCTGGCGCAGCGCACCCTCGGTTGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
ACGACATTGTGATTACCGAAACCGGCAACGAAAACCTCACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATCGAAGCGTTG
ATGGCTGCTGCGAGAAAGCA

Downstream 100 bases:

>100_bases
AGGCGGTTATTAAGGAATAAACAATGCAAAGTGTTGATGTAGCCATTGTTGGCGGCGGCATGGTGGGGCTGGCGGTTGCC
TGCGGCTTACAGGGTAGCGG

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVHV
SDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVAA
DGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDEK
FCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGE
DMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=29.0155440414508, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI32967307, Length=374, Percent_Identity=30.2139037433155, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=98.9795918367347, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1786883, Length=372, Percent_Identity=32.5268817204301, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.2798982188295, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=25.6470588235294, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=24.7240618101545, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920760, Length=420, Percent_Identity=23.8095238095238, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42339; Mature: 42208

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVAADGTHSALATVCGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEEHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVAADGTHSALATVCGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEEHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]