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The map label for this gene is yeiE

Identifier: 209399117

GI number: 209399117

Start: 3025078

End: 3025959

Strand: Reverse

Name: yeiE

Synonym: ECH74115_3293

Alternate gene names: 209399117

Gene position: 3025959-3025078 (Counterclockwise)

Preceding gene: 209396884

Following gene: 209397992

Centisome position: 54.31

GC content: 53.63

Gene sequence:

>882_bases
ATGCACATCACCCTCCGGCAGTTGGAAGTTTTTGCAGAAGTATTGAAAAGTGGATCAACCACCCAAGCGTCGGTGATGCT
GGCGTTGTCGCAATCAGCAGTGAGCGCAGCCTTGACCGACCTGGAAGGGCAGCTTGGCGTGCAACTGTTTGATCGCGTGG
GGAAAAGACTGGTTGTTAATGAACACGGGCGGCTGCTCTATCCGCGTGCGTTGGCATTGCTTGAACAGGCGGTTGAAATC
GAACAACTGTTTCGCGAAGACAACGGCGCGATTCGTATCTATGCCAGTAGTACCATCGGTAACTACATTCTGCCTGCAGT
TATCGCCCGTTATCGCCATGATTATCCGCAGTTGCCGATTGAACTTAGCGTTGGGAATAGCCAGGACGTGATGCAAGCGG
TGCTGGATTTCCGCGTTGATATTGGCTTTATTGAAGGACCGTGCCACAGCACTGAAATCATTTCTGAACCGTGGCTGGAA
GACGAGCTGGTGGTTTTCGCCGCGCCGACTTCGCCGTTGGCCCGTGGTCCGGTCACCTTAGAACAGCTGGCCGCTGCGCC
GTGGATCCTGCGTGAACGCGGTTCCGGCACGCGGGAGATTGTCGATTATCTGTTGCTGTCACATTTACCGAAGTTTGAGA
TGGCGATGGAATTAGGTAACTCCGAGGCAATCAAACATGCGGTGCGTCATGGGTTGGGAATTAGTTGCCTGTCGCGACGT
GTGATTGAAGATCAATTGCAGGCAGGCACATTAAGTGAAGTTGCGGTCCCTCTGCCGCGCCTGATGCGTACGTTGTGGCG
TATACATCATCGGCAAAAACACCTTTCCAACGCGCTACGGCGCTTTCTGGACTATTGCGATCCCGCAAATGTGCCGCGTT
AA

Upstream 100 bases:

>100_bases
ATAACCCTCTCCTTTAACCGGGCATAAGGTTACGACTAACTGGTTTAAAAATAAAATTGATTATATATTTATAATTAATC
TTTATAAGTGGTAAGCGACT

Downstream 100 bases:

>100_bases
GTTGCTGTACAAGAACATGCTGGTGCTGTGTCGATTTCGTGACGCAGCGCCTTCAGCATGCATTCGCCAGAAAAGAGATT
GGCTGCTTTACTTATAATCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR

Sequences:

>Translated_293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
>Mature_293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=25.8503401360544, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=27.681660899654, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787601, Length=198, Percent_Identity=29.2929292929293, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788296, Length=293, Percent_Identity=27.3037542662116, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=290, Percent_Identity=25.1724137931034, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787806, Length=180, Percent_Identity=31.1111111111111, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=206, Percent_Identity=29.6116504854369, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789639, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=279, Percent_Identity=27.2401433691756, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=25.0996015936255, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789506, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=25.2491694352159, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=24.8995983935743, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YEIE_ECO57 (P0ACR6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98010
- PIR:   C85854
- RefSeq:   NP_288740.1
- RefSeq:   NP_311076.1
- ProteinModelPortal:   P0ACR6
- SMR:   P0ACR6
- EnsemblBacteria:   EBESCT00000028705
- EnsemblBacteria:   EBESCT00000056050
- GeneID:   916753
- GeneID:   957119
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3414
- KEGG:   ecs:ECs3049
- GeneTree:   EBGT00070000031692
- HOGENOM:   HBG685425
- OMA:   QKHISKA
- ProtClustDB:   PRK10837
- BioCyc:   ECOL83334:ECS3049-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32724; Mature: 32724

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPI
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLARGPVTL
EEECCCCHHHHHHHHHHHHHCCEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCH
EQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPI
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLARGPVTL
EEECCCCHHHHHHHHHHHHHCCEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCH
EQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796