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The map label for this gene is fdoI

Identifier: 209399092

GI number: 209399092

Start: 4982973

End: 4983608

Strand: Reverse

Name: fdoI

Synonym: ECH74115_5342

Alternate gene names: 209399092

Gene position: 4983608-4982973 (Counterclockwise)

Preceding gene: 209398803

Following gene: 209400177

Centisome position: 89.44

GC content: 52.36

Gene sequence:

>636_bases
ATGAAACGACGTGACACCATCGTGCGCTACACCGCGCCGGAACGTATCAACCACTGGATCACCGCCTTCTGCTTCATCCT
GGCGGCGGTGAGCGGGCTGGGCTTTTTGTTCCCGTCCTTCAACTGGTTGATGCAAATCATGGGCACACCGCAGCTGGCGC
GAATTCTGCACCCGTTTGTCGGCGTGGTTATGTTTGCCTCGTTCATCATCATGTTTTTCCGTTACTGGCATCACAACCTA
ATCAATCGGGATGATATCTTTTGGGCGAAGAATATTCGTAAGATCGTCGTCAACGAGGAAGTAGGTGACACCGGGCGTTA
TAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGC
GTCCTTATTTTGCGCCTGCTTTCTCAATCCCGGTGATCCGATTCGCGTTAATGCTGCATTCATTTGCCGCAGTGGCGTTA
ATTGTGGTTATCATGGTGCATATCTACGCCGCCCTTTGGGTGAAAGGCACGATTACCGCCATGGTGGAAGGATGGGTTAC
CAGCGCTTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAAGACAACGGAAAAGAAAGCTGAATGA

Upstream 100 bases:

>100_bases
CTACCTTCGCAGCCAGTATCTTCCACTACGTGGGTGTCGGTCCGAACCATGCGGATGAGGAAGAGAATAATCTGCACGAA
GAGAAAGACGAGGAGCGCAA

Downstream 100 bases:

>100_bases
GTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCT
CGGCTCAAGAATTTATACAA

Product: formate dehydrogenase-O subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE

Sequences:

>Translated_211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
>Mature_211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family

Homologues:

Organism=Escherichia coli, GI1790325, Length=211, Percent_Identity=100, Blast_Score=429, Evalue=1e-122,
Organism=Escherichia coli, GI1787750, Length=213, Percent_Identity=47.887323943662, Blast_Score=213, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FDOI_ECO57 (P0AEL2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86078
- PIR:   B91231
- RefSeq:   NP_290521.1
- RefSeq:   NP_312845.1
- ProteinModelPortal:   P0AEL2
- SMR:   P0AEL2
- EnsemblBacteria:   EBESCT00000026423
- EnsemblBacteria:   EBESCT00000058977
- GeneID:   915078
- GeneID:   960260
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5434
- KEGG:   ecs:ECs4818
- GeneTree:   EBGT00050000010162
- HOGENOM:   HBG488114
- OMA:   SGLAMFH
- ProtClustDB:   PRK10639
- BioCyc:   ECOL83334:ECS4818-MONOMER
- GO:   GO:0006810
- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471
- TIGRFAMs:   TIGR01583

Pfam domain/function: PF00033 Cytochrom_B_N; SSF81342 Transmembr_di-haem_cytochrome

EC number: 1.2.1.2

Molecular weight: Translated: 24607; Mature: 24607

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13165464)-; HASH(0x132af668)-; HASH(0x12e03d88)-; HASH(0x10ee3920)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFV
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITA
HHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCH
>Mature Secondary Structure
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFV
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITA
HHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796