Click here to switch to the map view.

The map label for this gene is gadW [H]

Identifier: 209399020

GI number: 209399020

Start: 1182144

End: 1182731

Strand: Direct

Name: gadW [H]

Synonym: ECH74115_1164

Alternate gene names: 209399020

Gene position: 1182144-1182731 (Clockwise)

Preceding gene: 209399037

Following gene: 209399945

Centisome position: 21.22

GC content: 42.01

Gene sequence:

>588_bases
ATGCATTATGGCAAAGTTAAAATTTTCGATATAAACCATTCCATAGTAAGTCAATATCTGGAAATTCAGCATAAGCTGAC
AAGAACTCATCTGACTGACGTTCCGCTTTATCTGTCACTGGAACCCAACAACCCTGCGTTGGCTGAGGCTTTAATTACCA
GCCAGAGATTTTCCGGAGATACCACGGATATGTTTCTTATGATGGCATGCCTGTCGCTGTTTGAATCAGATGAACGGATA
TTATTATTTTTAAGTGGATGTTTATCCAGTATAAGTGCCAAAGTCAGGGCGATAATTCAGACAGATATATCAGCAAGCTG
GACGCTTGGTGCGATTGCGTTACGCCTGCATATGAGTGAGAGTTTGTTAAAGATAAAACTGAAAAATGAAGGGCACATGT
TCAGTCGCTTGTTGCTGGAAGAGCGGATGCGTGTTGCTGTCAATATGTTATGTTCCCGGCATGGATATGGACAGGCTGTA
GCAGAAAAATGCGGTTATTCAAGCTGGTCCTACTTTATTTCTGTATTTCACCGCTATTATGGCTTCCCGCCAGACAGATA
TGTATCCAGGCAAGGGCTTGATTATTGA

Upstream 100 bases:

>100_bases
GGTTTGCTCCCCAATTAATATTTTTCTTGAAAAGGATACGTTGTCACTTAAGCCCGGCTCAGTCGTTCTGGCCACCAAAT
GCATCAGGGCGCTTTTCCTT

Downstream 100 bases:

>100_bases
TTTTCATCTGATTATTATTTTTTGACCCAGCCCTTTAGCTCAGTGGTGAGAGCGAGCGACTCATAATCGCCAGGTCGCTG
GTTCAAATCCAGCAAGGGCC

Product: putative envelope protein encoded within prophage CP-933N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERI
LLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAV
AEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY

Sequences:

>Translated_195_residues
MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERI
LLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAV
AEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY
>Mature_195_residues
MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERI
LLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAV
AEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=186, Percent_Identity=37.0967741935484, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787776, Length=126, Percent_Identity=40.4761904761905, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1786776, Length=192, Percent_Identity=29.6875, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789933, Length=154, Percent_Identity=38.3116883116883, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790557, Length=193, Percent_Identity=33.160621761658, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786778, Length=127, Percent_Identity=44.8818897637795, Blast_Score=84, Evalue=4e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 22296; Mature: 22296

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCC
TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
SLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYY
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHH
GFPPDRYVSRQGLDY
CCCCHHHHCCCCCCC
>Mature Secondary Structure
MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCC
TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
SLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYY
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHH
GFPPDRYVSRQGLDY
CCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]