Click here to switch to the map view.

The map label for this gene is kgtP [H]

Identifier: 209398967

GI number: 209398967

Start: 3548762

End: 3550141

Strand: Reverse

Name: kgtP [H]

Synonym: ECH74115_3823

Alternate gene names: 209398967

Gene position: 3550141-3548762 (Counterclockwise)

Preceding gene: 209397795

Following gene: 209397290

Centisome position: 63.71

GC content: 48.26

Gene sequence:

>1380_bases
ATGTATAACATTTTCTTTGCCAAAAGAAATAAACAAAAGCGACCGACAAAAGCATCGGATTACGGCAGGAGACATAATGG
CATGGCTGAAAGTACTGTAACGGCAGACAGCAAACTGACAAGTAGTGATACTCGTCGCCGCATTTGGGCGATTGTGGGGG
CCTCTTCAGGTAATCTGGTCGAGTGGTTCGATTTCTATGTCTACTCGTTCTGTTCACTCTACTTTGCCCACATCTTCTTC
CCTTCCGGGAACACGACGACTCAACTACTACAAACAGCAGGTGTTTTTGCTGCGGGATTCCTGATGCGCCCAATAGGCGG
TTGGCTATTTGGCCGCATAGCCGATAAACATGGTCGCAAAAAATCGATGCTGCTATCGGTGTGTATGATGTGTTTCGGCT
CACTGGTAATCGCCTGTTTGCCTGGTTATGAAACAATCGGTACGTGGGCTCCGGCATTATTGCTTCTCGCGCGTTTATTT
CAGGGATTATCCGTTGGCGGAGAATATGGCACCAGCGCCACCTATATGAGTGAAGTTGCCGTTGAAGGGCGCAAAGGTTT
TTACGCATCATTTCAGTATGTGACGTTGATCGGCGGACAACTGCTAGCCCTACTGGTTGTCGTGGTTTTACAACACACCA
TGGAAGACTCTGCACTCAGAGAGTGGGGATGGCGTATTCCTTTCGCGTTAGGAGCTGTGTTAGCTGTTGTGGCGTTGTGG
TTACGTCGTCAGTTAGATGAAACTTCGCAACAAGAAACGCGCGCTTTAAAAGAAGCTGGATCTCTGAAAGGATTATGGCG
CAATCGCCGTGCATTCATCATGGTTCTCGGTTTTACCGCTGCGGGCTCCCTTTGTTTCTATACCTTCACCACTTATATGC
AGAAGTATCTGGTAAATACTGCGGGAATGCATGCCAACGTGGCGAGTGGCATTATGACTGCCGCATTGTTTGTATTCATG
CTTATTCAACCACTCATTGGCGCGCTGTCGGATAAGATTGGTCGCCGTACCTCAATGTTATGTTTCGGTTCGCTGGCAGC
CATTTTTACCGTTCCTATTCTCTCAGCATTGCAAAACGTTTCCTCGCCTTATGCCGCTTTTGGTCTGGTGATGTGTGCCC
TGCTGATAGTGAGTTTTTATACATCAATCAGTGGAATACTGAAGGCTGAGATGTTCCCGGCACAGGTTCGCGCATTAGGC
GTTGGTCTGTCATATGCGGTCGCTAATGCTATATTTGGTGGTTCGGCGGAGTACGTAGCGTTGTCGCTGAAATCAATAGG
AATGGAAACAGCCTTCTTCTGGTATGTGACCTTGATGGCCGTGGTGGCGTTTCTGGTTTCTTTGATGCTACATCGCAAAG
GGAAGGGGATGCGTCTTTAG

Upstream 100 bases:

>100_bases
ATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGCTCCTCAATAACCATTTTCCTGCTAACTAAATTCATGGTTAAGGT
TGCATAATGATACGTAACAA

Downstream 100 bases:

>100_bases
TGACGGGTCAGTTGCCAGACGGTATAGCCGGTGCTTGCACCGGCGACATCCCAGGCCAAATCCTTCCAGCTCCAGCCGCT
CCCTTCGGGGCGGCTATCCC

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MYNIFFAKRNKQKRPTKASDYGRRHNGMAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFF
PSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLF
QGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALW
LRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFM
LIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALG
VGLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL

Sequences:

>Translated_459_residues
MYNIFFAKRNKQKRPTKASDYGRRHNGMAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFF
PSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLF
QGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALW
LRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFM
LIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALG
VGLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL
>Mature_459_residues
MYNIFFAKRNKQKRPTKASDYGRRHNGMAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFF
PSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLF
QGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALW
LRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFM
LIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALG
VGLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=432, Percent_Identity=99.7685185185185, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=29.7727272727273, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=31.25, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=31.25, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI87082231, Length=320, Percent_Identity=25.625, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.3455497382199, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50336; Mature: 50336

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNIFFAKRNKQKRPTKASDYGRRHNGMAESTVTADSKLTSSDTRRRIWAIVGASSGNLV
CCEEEEECCCCCCCCCCHHHCCHHCCCCCHHHCCCCCCCCCCHHHHEEEEEEECCCCCHH
EWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
VEGRKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALW
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AGMHANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
SSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYNIFFAKRNKQKRPTKASDYGRRHNGMAESTVTADSKLTSSDTRRRIWAIVGASSGNLV
CCEEEEECCCCCCCCCCHHHCCHHCCCCCHHHCCCCCCCCCCHHHHEEEEEEECCCCCHH
EWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
VEGRKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALW
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AGMHANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
SSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]