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The map label for this gene is cas3 [H]

Identifier: 209398890

GI number: 209398890

Start: 3708787

End: 3711486

Strand: Reverse

Name: cas3 [H]

Synonym: ECH74115_4014

Alternate gene names: 209398890

Gene position: 3711486-3708787 (Counterclockwise)

Preceding gene: 209399654

Following gene: 209395699

Centisome position: 66.61

GC content: 50.33

Gene sequence:

>2700_bases
ATGCGTAAATATCCTTTAAGTTTACTGAAGGATAAAAATATTGTGACTTTCTTTGATTTCTGGGGAAAAACCCGACGTGG
CGAGAAAGAGGGTGGCGACGGCTATCACCTTCTTTGCTGGCATTCGCTGGATGTGGCCGCAATGGGCTATTTAATGGTTA
AAAGAAATTGCTTCGGGCTGGCTGATTACTTTCGTCAATTAGGGATTTCTGACAAGGAACAGGCGGCTCAATTTTTCGCT
TGGTTGCTGTGCTGGCACGATATTGGAAAATTTGCCCGCTCTTTTCAGCAACTTTACCTGGCCCCTGAACTCAAGATTCC
GGAAGGTTCCAGAAAGAATTACGAAAAGATCTCTCATTCAACGCTGGGTTACTGGCTGTGGAATTATTATTTAAGTGAAT
GTGAGGAGTTGCTTCCTTCATCTTCACTCTCTTCTCGTAAACTTACACGTGTAATAGAGATGTGGATGTCCATAACTACC
GGGCATCATGGTCGACCACCTGACCGTATTGATGAGCTGGATAATTTTCTGCCTGAAGACAAAGCTGCCGCGCGAGATTT
TCTCCTTGAAATCAAGGCACTGTTTCCGCTCATAGAGATTCCCACATTCTGGGATGATGACGAGGGCGTTGAACTTTTAA
AACAACTTTCCTGGTATATCTCTGCAACAGTCGTACTCGCAGACTGGACGGGTTCGTCAACGCGATTTTTTCCACGCGTC
GCACACCCAATGGATATTAAAGATTACTGGCAGAAAACTTTAGTTCAGGCTCAAAACGCCTTAACCGTCTTTCCTCCAAA
AGCAGAAACCGCACCTTTCACCGGAATTAATACGCTGTTTCCTTTTATTGAGCACCCGACACCATTACAGCAAAAGGTAC
TGGATCTGGATATCAGCCAGCCAGGGCCACAGTTATTTATTCTGGAAGACGTGACTGGCGCAGGTAAAACAGAAGCGGCG
CTTATCCTGGCGCACAGGTTGATGGCTGCGAGGAAAGCACAGGGTTTGTTTTTTGGCCTGCCAACAATGGCAACGGCCAA
TGCCATGTACGATCGGCTGGTCAAAACCTGGCTTGCTTTCTATTCGCCAGAGTCCCGCCCCAGCTTGGTGCTGGCACACA
GTGCCCGCACATTAATGGACCGCTTCAATGAATCACTCTGGTCCGGTGATTTAGTCGGGTCAGAAGAACCGGATGAACAA
ACATTCAGTCAGGGATGTGCGGCCTGGTTTGCCAACAGTAACAAGAAGGCGCTACTGGCTGAAATTGGCGTCGGCACGCT
GGATCAGGCGATGATGGCAGTGATGCCGTTTAAACATAATAATCTGCGGCTTCTGGGGTTGAGTAACAAAATCCTGCTGG
CTGATGAGATCCATGCCTGTGATGCTTACATGTCGTGCATTCTTGAAGGGCTGATCGAGCGGCAGGCGCGTGGCGGAAAC
AGCGTCATTTTGCTTTCTGCTACGTTATCCCAACAGCAGCGCGACAAACTCGTCGCCGCCTTTGCGCGTGGCACAGAGGG
CCAGCAAGAAGCTCCGTTCCTTGAAAAGGATGATTACCCCTGGCTGACGCATGTCACGAAATCCGATGTGAACTCACACC
GGGTAGCGACGCGCAAAGACGTTGAGCGTAGCGTCAGCGTGGGTTGGCTTCATAGTGAACAAGAGAGTATTGCGCGTATC
GAATCGGCGGTAAGTCAGGGAAAATGCATCGCCTGGATCCGGAATTCTGTCGATGACGCTATTAAGGTTCATCGTCAGCT
GCTTGCCCGCGGCGTCATTCCCGCTTCCAGCCTTTCACTCTTTCATAGCCGCTTTGCTTTTAGCGATCGCCAGCGAATTG
AAATGGAGACGCTGGCACGCTTTGGTAAAGAAGACGGTTCACAGCGTGCCGGAAAAGTCCTCATTTGTACTCAGGTCTTA
GAGCAGAGCGTTGATTGTGACCTGGACGAAATGATCTCCGACCTGGCCCCTGTTGATTTGCTGATTCAGCGAGCGGGGCG
ATTACAGCGGCATATCCGCGATATTAATGGTCAGTTAAAGCGTGACGGAAAAGACGAGCGTTCCCCTCCTGAATTGCTGA
TTCTGGCCCCCGTCTGGGACGACGCTCCTGGTGACGAATGGTTCGGCAGTGCCATGCGTAACAGTGCATATGTCTATCCC
GATCATGGACGAATCTGGCTGACGCAGCGTGTACTGCGTGAGCAAGGCGCTATTCAAATGCCACACGCAGCCCGCCTTCT
TATTGAATCAGTCTACGGTGAGGACGTGGTAATGCCGGAAGGATTTGCCCGCAGCGAGCAGGAGCAAGTGGGCAAATATT
ACTGCGATCGCGCAATGGCTAAAAAGTTTGTCCTGAACTTCAAGCCTGGCTATGCCGCCAATATCAACGATTACCTTCCG
GAAAAGCTGTCGACACGTCTGGCTGAGGAATCTGTTTCCCTGTGGCTGGCTACCTGTATTGCCGGTGTGGTGAAGCCTTA
TGCCACCGGTGCTCACGCATGGGAAATGAGCGTTGTCAGAGTGCGTCGAAGCTGGTGGAAAAAACATCGGGATGAGTTTT
CTTTACTGGAAGGGGAAGCGTTCAGGCAGTGGTGCATTGAACAGCGGCAAGATCCGGAAATGGCAAACGTGATTTTAGTC
ACTGATGACGAAAGTTGCGGGTATTCGGCCAGGGAGGGATTGATTGGCAAGGTTGATTGA

Upstream 100 bases:

>100_bases
TAGTCTCTATTTATCATTATGAATTTCAAATGAAAATCATTAATTTATTCATAATATAAATGTTTATTGGCTGATATATA
TCACAGATAATTTTTAAAAT

Downstream 100 bases:

>100_bases
GATACCTGCGACAGGACTCTCTGAATAGTTGATACACCGAAAAGGATGGAATGATGGACAACCGACCAGGGATGGTCTTT
TTTACGAGAGGATACATATG

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 899; Mature: 899

Protein sequence:

>899_residues
MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFA
WLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRV
AHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAA
LILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQ
TFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGN
SVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARI
ESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVL
EQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYP
DHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLP
EKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILV
TDDESCGYSAREGLIGKVD

Sequences:

>Translated_899_residues
MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFA
WLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRV
AHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAA
LILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQ
TFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGN
SVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARI
ESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVL
EQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYP
DHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLP
EKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILV
TDDESCGYSAREGLIGKVD
>Mature_899_residues
MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFA
WLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRV
AHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAA
LILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQ
TFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGN
SVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARI
ESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVL
EQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYP
DHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLP
EKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILV
TDDESCGYSAREGLIGKVD

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789119, Length=822, Percent_Identity=28.5888077858881, Blast_Score=264, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006483
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 101878; Mature: 101878

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGL
CCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
ADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
TLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDNFLPED
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
KAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCC
AHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQ
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCC
PGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWLAF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHH
YSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFANSNKKALLA
HCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
EIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGN
CCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
SVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKD
EEEEEEEHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
VERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSL
HHHHCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHHCCCCHHHHHHHHCHHHH
LIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYP
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEC
DHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMA
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
KKFVLNFKPGYAANINDYLPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVR
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGL
CCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
ADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISHS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
TLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDNFLPED
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
KAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTRFFPRV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCC
AHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKVLDLDISQ
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCC
PGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAMYDRLVKTWLAF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHH
YSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFANSNKKALLA
HCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
EIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGN
CCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
SVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKD
EEEEEEEHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
VERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSL
HHHHCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHHCCCCHHHHHHHHCHHHH
LIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYP
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEC
DHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMA
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
KKFVLNFKPGYAANINDYLPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVR
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2005873; 7984428 [H]