Click here to switch to the map view.

The map label for this gene is imp [H]

Identifier: 209398538

GI number: 209398538

Start: 59726

End: 62047

Strand: Reverse

Name: imp [H]

Synonym: ECH74115_0060

Alternate gene names: 209398538

Gene position: 62047-59726 (Counterclockwise)

Preceding gene: 209396031

Following gene: 209398433

Centisome position: 1.11

GC content: 51.34

Gene sequence:

>2322_bases
ATGATTGCCACCGCCCTTTATAGTCAACAGGGACTGGCAGCCGACCTCGCCTCACAGTGCATGTTGGGCGTGCCAAGCTA
TGACCGTCCTCTGGTACAGGGCGATACCAATGACTTACCCGTGACTATCAATGCTAACCACGCGAAAGGGGACTACCCGG
ATGACGCCGTGTTTACTGGCAGCGTGGATATCATGCAGGGTAACAGCCGTCTGCAGGCCGACGAAGTGCAGCTCCATCAA
AAAGAGGCACCAGGACAACCGGAGCCGGTACGTACCGTTGATGCGCTCGGTAATGTCCATTACGACGATAACCAGGTGAT
CCTCAAAGGGCCGAAAGGCTGGGCGAATCTGAACACCAAAGATACCAACGTCTGGGAAGGTGATTACCAGATGGTGGGTC
GCCAGGGTCGCGGTAAAGCGGACCTGATGAAACAACGTGGCGAAAACCGCTATACCATTCTGGATAACGGTAGCTTTACC
TCCTGTCTGCCGGGTTCTGACACCTGGAGCGTGGTAGGTAGCGAAATTATTCATGACCGCGAAGAACAAGTTGCGGAGAT
CTGGAACGCCCGCTTTAAGGTGGGTCCGGTATCGATCTTTTATAGCCCCTATTTGCAGTTGCCGGTGGGTGACAAACGTC
GCTCTGGTTTCTTGATCCCGAACGCCAAGTACACCACCACCAACTACTTTGAGTTCTACCTGCCATATTACTGGAACATC
GCGCCAAATATGGATGCCACCATCACGCCGCATTATATGCATCGTCGTGGCAACATCATGTGGGAGAACGAATTCCGCTA
CCTCTCCCAGGCGGGCGCTGGCTTGATGGAACTGGACTATCTGCCTTCAGATAAAGTCTATGAAGATGAACACCCGAACG
ATGACAGTTCACGTCGTTGGTTATTCTACTGGAACCACTCCGGGGTCATGGATCAGGTGTGGCGTTTCAACGTCGACTAC
ACCAAGGTCAGCGATCCTAGCTACTTCAATGATTTCGATAACAAGTACGGTTCCAGTACTGACGGCTACGCAACGCAAAA
ATTCAGCGTTGGCTATGCGGTGCAAAACTTCAATGCCACCGTTTCAACCAAGCAGTTCCAGGTTTTCAGCGAACAGAACA
CCAGTAGCTACTCGGCAGAGCCGCAGTTAGACGTTAATTACTACCAGAATGATGTTGGTCCGTTTGATACGCGTATTTAC
GGCCAGGCAGTGCACTTTGTTAACACCAGAGACGACATGCCTGAAGCAACCCGTGTTCACCTGGAACCGACCATCAATTT
GCCGCTCTCTAATAACTGGGGCAGCATCAATACCGAAGCGAAGTTGCTGGCAACCCATTATCAGCAAACCAATCTTGACT
GGTATAACTCCAGAAACACGACCAAGCTGGACGAATCCGTTAACCGCGTAATGCCGCAATTCAAAGTTGACGGCAAAATG
GTCTTTGAACGCGATATGGAAATGCTGGCTCCGGGTTATACCCAAACGCTGGAACCGCGCGCGCAGTATTTGTACGTGCC
GTATCGCGATCAGAGCGACATCTATAACTACGACTCGTCTCTGCTGCAATCTGACTACTCTGGCCTGTTCCGGGACCGGA
CTTACGGCGGTCTTGACCGTATTGCCTCCGCTAACCAGGTGACGACCGGTGTCACATCTCGCATATATGATGATGCTGCC
GTTGAACGTTTTAATATTTCCGTTGGTCAAATCTACTATTTCACGGAGTCTCGCACTGGCGATGACAACATAACATGGGA
GAATGACGACAAAACGGGTTCACTGGTGTGGGCAGGCGATACTTACTGGCGTATCTCCGAGCGTTGGGGATTGCGTGGCG
GGATTCAGTACGATACACGTCTGGATAACGTAGCGACCAGTAACTCCAGCATTGAATACCGTCGGGATGAAGACCGTCTG
GTACAGCTGAATTACCGTTACGCCAGCCCGGAATATATTCAGGCTACGCTGCCTAAGTACTATTCCACTGCTGAGCAATA
TAAGAATGGTATTTCGCAGGTAGGTGCTGTCGCCAGCTGGCCAATTGCCGATCGTTGGTCCATTGTTGGGGCCTACTACT
ACGACACCAATGCGAACAAGCAAGCCGACTCTATGTTAGGTGTGCAATACAGCTCCTGCTGCTATGCAATTCGCGTCGGT
TACGAGCGGAAGCTGAACGGTTGGGATAACGATAAACAACATGCGGTATATGACAACGCAATCGGCTTTAACATCGAACT
TCGCGGCCTGAGCTCCAACTACGGTCTGGGTACGCAAGAGATGCTGCGTTCGAACATTCTGCCGTATCAAAACACTTTGT
GA

Upstream 100 bases:

>100_bases
GAGTACGTTAGTCTCTGACCGTTTGTCACGCGCAACGTTACCGATGATGGAACAATAAAATCAACGTATGAAAAAACGTA
TCCCCACTCTCCTGGCCACC

Downstream 100 bases:

>100_bases
TTTGTTGATTTACCACGTAATCCGCAGTGCGGTTAATTGAAATGGAAAAAGTATGAAGAACTGGAAAACGCTGCTTCTCG
GTATCGCCATGATCGCGAAT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQ
KEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFT
SCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNI
APNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDY
TKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIY
GQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKM
VFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRIYDDAA
VERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRL
VQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL

Sequences:

>Translated_773_residues
MIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQ
KEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFT
SCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNI
APNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDY
TKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIY
GQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKM
VFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRIYDDAA
VERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRL
VQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
>Mature_773_residues
MIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQ
KEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFT
SCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNI
APNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDY
TKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIY
GQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKM
VFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRIYDDAA
VERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRL
VQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=773, Percent_Identity=99.7412677878396, Blast_Score=1600, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88407; Mature: 88407

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINANHAKGDYPDDAVFTG
CCEEEEECCCCHHHHHHHHHEECCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCEEEC
SVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTK
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEECCCCCCCCCCC
DTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTWSVVGSEIIHDR
CCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHCH
EEQVAEIWNARFKVGPVSIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNI
HHHHHHHHCCEEEECEEEEEECCEEECCCCCCCCCCEECCCCCEEECCEEEEEEEEEEEE
APNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRW
CCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEE
LFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNAT
EEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEEEEEEEECCCCE
VSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVH
EEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEE
LEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKM
EEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHHHCCCEECCCEE
VFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDR
EEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
IASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGD
HCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCCCCCCCEEECCC
TYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKY
CEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHHH
YSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
HHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEEECCCCCCHHHHHCCCHHCEEEEEEEC
YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
CEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINANHAKGDYPDDAVFTG
CCEEEEECCCCHHHHHHHHHEECCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCEEEC
SVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTK
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEECCCCCCCCCCC
DTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTWSVVGSEIIHDR
CCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHCH
EEQVAEIWNARFKVGPVSIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNI
HHHHHHHHCCEEEECEEEEEECCEEECCCCCCCCCCEECCCCCEEECCEEEEEEEEEEEE
APNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRW
CCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEE
LFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNAT
EEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEEEEEEEECCCCE
VSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVH
EEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEE
LEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKM
EEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHHHCCCEECCCEE
VFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDR
EEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
IASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGD
HCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCCCCCCCEEECCC
TYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKY
CEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHHH
YSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
HHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEEECCCCCCHHHHHCCCHHCEEEEEEEC
YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
CEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA