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The map label for this gene is mdtE

Identifier: 209398509

GI number: 209398509

Start: 4504444

End: 4505601

Strand: Direct

Name: mdtE

Synonym: ECH74115_4873

Alternate gene names: 209398509

Gene position: 4504444-4505601 (Clockwise)

Preceding gene: 209400509

Following gene: 209399072

Centisome position: 80.84

GC content: 54.06

Gene sequence:

>1158_bases
ATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTGCGGCGCGATGCTCACCGCCTGCGATGACAAATCGGCGGA
AAACACCGCCGCCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCTGGGTCGGTCAATGTGTTGAGCGAATTGCCCG
GTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGC
GATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTC
GCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACG
TTAGCCGTCAGGATTACGACACCGCGCGCACCCAGTTGAATGAAGCAGAAGCCAATGTCACCGTCGCCAAAGCGGCTGTT
GAACAGGCGACGATCAATCTGCAATACGCGAATGTCACCTCGCCGATTACGGGCGTCAGCGGGAAATCGTCGGTGACCGT
CGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGC
AGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTA
CAGCTCAATCTGGAAAATGGTAAACGTTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACGGTTGATGAAACCAC
GGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTTGCTGCCTGGCATGTATGTCACGGCATTAGTGG
ATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAAGGTAAAGCAACGGCGCTC
ATTCTGGATAAAGACGATGTCGTGAAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGTTGTCACCTC
TGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCT
CCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAA

Upstream 100 bases:

>100_bases
GCTGACAACCCCACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTC
AAGAATTTTCAGGGACTAAA

Downstream 100 bases:

>100_bases
CGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTAT
GATGCTTGCAGGTGGTCTGG

Product: multidrug efflux system protein MdtE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVKLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ

Sequences:

>Translated_385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVKLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ
>Mature_385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVKLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=99.4805194805195, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI1786668, Length=382, Percent_Identity=54.4502617801047, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789665, Length=382, Percent_Identity=49.2146596858639, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI87082036, Length=311, Percent_Identity=28.6173633440514, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081796, Length=346, Percent_Identity=24.2774566473988, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081951, Length=130, Percent_Identity=35.3846153846154, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTE_ECO57 (Q8X4L0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91178
- PIR:   B86024
- RefSeq:   NP_290093.1
- RefSeq:   NP_312420.1
- ProteinModelPortal:   Q8X4L0
- SMR:   Q8X4L0
- EnsemblBacteria:   EBESCT00000026719
- EnsemblBacteria:   EBESCT00000055646
- GeneID:   915750
- GeneID:   961155
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4926
- KEGG:   ecs:ECs4393
- GeneTree:   EBGT00050000009284
- HOGENOM:   HBG742930
- OMA:   DDEARIT
- ProtClustDB:   PRK09859
- BioCyc:   ECOL83334:ECS4393-MONOMER
- InterPro:   IPR006143
- TIGRFAMs:   TIGR01730

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 41221; Mature: 41221

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCHHHHHCCCCCCCCH
VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARI
HHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEE
TFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVS
EECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHEEEEEEEECCCCCCCCCC
GKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
CCCCEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQN
EEEECCCCEECCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
VLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQAGDRVIVSGL
EEEECCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
QRIRPGIKARAISSSQENASTESKQ
HHHCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCHHHHHCCCCCCCCH
VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARI
HHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEE
TFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVS
EECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHEEEEEEEECCCCCCCCCC
GKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
CCCCEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQN
EEEECCCCEECCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
VLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQAGDRVIVSGL
EEEECCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
QRIRPGIKARAISSSQENASTESKQ
HHHCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796