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The map label for this gene is ygcF

Identifier: 209398503

GI number: 209398503

Start: 3728952

End: 3729623

Strand: Reverse

Name: ygcF

Synonym: ECH74115_4033

Alternate gene names: 209398503

Gene position: 3729623-3728952 (Counterclockwise)

Preceding gene: 209400352

Following gene: 209397522

Centisome position: 66.93

GC content: 51.49

Gene sequence:

>672_bases
ATGCAGTACCCGATTAACGAGATGTTCCAGACCCTGCAAGGTGAGGGTTACTTTACCGGCGTCCCCGCCATTTTTATTCG
TTTACAGGGATGCCCGGTTGGCTGTGCCTGGTGCGACACCAAACACACCTGGGAAAAGCTTGAGGATCGGGAAGTCTCCC
TTTTCAGCATTCTGGCGAAGACCAAAGAGAGTGATAAGTGGGGGGCGGCGAGCAGTGAAGACTTGCTGGCTGTCATTGGT
CGCCAGGGATATACCGCGCGGCATGTGGTGATTACGGGTGGTGAGCCTTGCATTCATGATTTGCTGCCACTGACTGACCT
GCTCGAAAAGAACGGTTTTAGCTGCCAGATCGAAACCAGTGGTACTCATGAAGTACGCTGTACACCGAATACCTGGGTTA
CCGTATCGCCAAAGCTGAACATGCGCGGCGGCTATGAAGTGTTGTCACAGGCACTGGAGCGAGCCAACGAAATCAAGCAT
CCAGTGGGACGCGTACGTGATATTGAAGCACTGGATGAACTTTTGGCGACGCTGACCGATGATAAACCGCGAGTCATTGC
ACTGCAGCCGATTAGCCAAAAGGATGATGCCACACGTTTGTGCATTGAAACCTGCATTGCGCGTAATTGGCGTTTGTCGA
TGCAAACCCATAAATATTTAAATATTGCCTGA

Upstream 100 bases:

>100_bases
CTTTTTGCCATAAACCTGCCTGTAACAGAATGTTAATAAGACATGATTGTTGTGGCAGGATCTGCAATAATTAACAATTA
TCCACTTCACAGAGAATGCT

Downstream 100 bases:

>100_bases
TTAAACATTTATAAGCGTTATAAATGGGTGGAACCTGTAAAGACTTCTACTCATTTATATTCTTTGTCGTCTCTGAAAAC
TCATCCATCTTGCCTATCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIG
RQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA

Sequences:

>Translated_223_residues
MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIG
RQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA
>Mature_223_residues
MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIG
RQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA

Specific function: Unknown

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily

Homologues:

Organism=Escherichia coli, GI1789139, Length=223, Percent_Identity=100, Blast_Score=466, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGCF_ECO57 (P64555)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91083
- RefSeq:   NP_289328.1
- RefSeq:   NP_311660.1
- ProteinModelPortal:   P64555
- EnsemblBacteria:   EBESCT00000024387
- EnsemblBacteria:   EBESCT00000058052
- GeneID:   912281
- GeneID:   958233
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4089
- KEGG:   ecs:ECs3633
- GeneTree:   EBGT00050000009993
- HOGENOM:   HBG507714
- OMA:   TICLQPI
- ProtClustDB:   CLSK874339
- BioCyc:   ECOL83334:ECS3633-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 25030; Mature: 25030

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAK
CCCCHHHHHHHHCCCCEEECCCEEEEEECCCCCEEEECCCHHHHHHHCCCHHHHHHHHHH
TKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETS
CCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECC
GTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKHPVGRVRDIEALDELLATLTD
CCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEECC
>Mature Secondary Structure
MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAK
CCCCHHHHHHHHCCCCEEECCCEEEEEECCCCCEEEECCCHHHHHHHCCCHHHHHHHHHH
TKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETS
CCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECC
GTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKHPVGRVRDIEALDELLATLTD
CCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796