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The map label for this gene is bcr [H]

Identifier: 209398488

GI number: 209398488

Start: 3054862

End: 3056052

Strand: Reverse

Name: bcr [H]

Synonym: ECH74115_3320

Alternate gene names: 209398488

Gene position: 3056052-3054862 (Counterclockwise)

Preceding gene: 209400555

Following gene: 209396240

Centisome position: 54.85

GC content: 53.48

Gene sequence:

>1191_bases
GTGACCACCCGACAGCATTCGTCGTTTGCTATTGTTTTTATCCTTGGCCTGCTGGCCATGTTGATGCCGCTGTCGATTGA
TATGTATCTGCCCGCGCTACCGGTAATTTCAGCGCAGTTTGGCGTACCGGCGGGCAGTACGCAGATGACCCTCAGTACTT
ATATTCTGGGCTTTGCGTTGGGGCAGTTAATCTACGGGCCGATGGCAGACAGCTTCGGGCGTAAGCCGGTGGTTCTTGGC
GGTACGCTGGTGTTTGCCGCCGCCGCGGTGGCGTGTGCGTTGGCACAAACCATCGATCAGCTGATTGTGATGCGTTTCTT
CCACGGGCTGGCGGCGGCTGCGGCCAGCGTGGTTATCAACGCTTTGATGCGTGATATCTACCCGAAAGAAGAGTTCTCGC
GGATGATGTCGTTTGTCATGCTGGTGACGACCATTGCACCGCTGATGGCACCGATAGTTGGCGGCTGGGTGCTGGTGTGG
CTGAGCTGGCATTACATCTTCTGGATCCTCGCCGTGGCGGCGATTCTGGCTTCGGCAATGATCTTCTTTCTGATCAAAGA
AACCTTACCACCGGAGCGTCGCCAGCCTTTTCACATTCGTACCACTATTGGTAACTTTGCGGCGCTGTTCCGTCATAAGC
GTGTCCTGAGTTATATGCTGGCCAGTGGTTTCAGCTTTGCCGGGATGTTCTCGTTCTTAAGCGCCGGACCGTTTGTCTAC
ATTGAAATTAACCACGTCGCGCCGGAAAACTTTGGTTATTACTTTGCACTAAACATTGTTTTCCTGTTCGTGATGACCAT
CTTTAATAGCCGCTTCGTCCGCCGTATTGGCGCGTTAAATATGTTCCGCTCGGGGTTGTGGATACAATTTATTATGGCAG
CGTGGATGGTCATCAGTGCGCTGCTGGGTCTGGGATTTTGGTCGCTGGTGGTTGGCGTTGCGGCGTTTGTGGGCTGCGTG
TCGATGGTGTCATCCAATGCGATGGCGGTCATTCTTGATGAGTTTCCCCATATGGCGGGAACGGCATCTTCGCTGGCAGG
AACCTTCCGTTTTGGCATAGGGGCAATTGTTGGCGCATTGCTTTCTCTTGCGACCTTTAACTCTGCATGGCCGATGATTT
GGTCAATTGCATTCTGCGCAACCAGCTCCATTCTCTTCTGTCTGTACGCCAGTCGGCCGAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TACGCTGGATGCTGATTTAGCCCCCGGTGAATATCGTCCGTTAACTGAAGAAGAAATTGCCAGCGTCGTCTAACTTTTCA
ATGACTTTCAGGAGCCCGTT

Downstream 100 bases:

>100_bases
TCTATTGCACAACGAGGAAGCTAAAAGGCTTCCTTTGTTGATGCATGTCAACCACAAATCTATCATTCCCCCGATATATG
TTTATTTTATGTAAAATCAA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR

Sequences:

>Translated_396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR
>Mature_395_residues
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGG
TLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWL
SWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYI
EINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVS
MVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=99.4949494949495, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=337, Percent_Identity=29.080118694362, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790146, Length=226, Percent_Identity=30.9734513274336, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082312, Length=337, Percent_Identity=26.1127596439169, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787065, Length=303, Percent_Identity=24.0924092409241, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790794, Length=284, Percent_Identity=20.4225352112676, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081738, Length=357, Percent_Identity=25.4901960784314, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787880, Length=209, Percent_Identity=29.6650717703349, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=26.6272189349112, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=25.6281407035176, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325414, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319517, Length=205, Percent_Identity=22.9268292682927, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=182, Percent_Identity=22.5274725274725, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43353; Mature: 43222

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILAVAAILASAM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]