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The map label for this gene is tnpR [H]

Identifier: 209398462

GI number: 209398462

Start: 5426793

End: 5427431

Strand: Direct

Name: tnpR [H]

Synonym: ECH74115_5792

Alternate gene names: 209398462

Gene position: 5426793-5427431 (Clockwise)

Preceding gene: 209400288

Following gene: 209396646

Centisome position: 97.39

GC content: 52.43

Gene sequence:

>639_bases
TTGGCTTTTCCGGCAGGCCATCCTATTTTCATCACCGTTTTCTGGCAGGGGCCGGATGAGGAGATGACTATGGCTATTTT
TGGCTACGGGCGTGTTTCGACATCCCAGCAGGATACTGAGAACCAGCGCATGGAACTTGAACAAGCTGGGTGGACGTTCG
ACTTTTGGTTTTCTGATGTAGTCAGCGGAAAGGTTCCTGCGGTGCAACGCAAAGCGTTTTTTGAAATGCTCAGCAAAATC
CGCGATGGGGAAACGTTGGTAGTTGCAAAGCTGGACCGCCTCGGGCGTGATGCAATTGACGTTCTGCAAACTGTCAGGAC
GTTGGCTGACCGAAACATCAAGGTTATTGTCCATCAGCTTGGCACCACTGATCTCACCTCTGCTGCCGGTAAGCTCCTGT
TGTCGATGCTAGCAGCGGTTGCGGAGATGGAGCGTGACCTGCTGATTGAACGGACTAATGCGGGGCTGTTACGGGCAAAA
GCTGAAGGCAGGAAATTGGGCAGGCCTGCTAAAATCGCACCGGAAGCTCGTGGAGCGATTTTAGACAAAAGGGCCGCTGG
GGTTAGTGTTAGCGCATTGGCGCGTGAATACGGTGTTTCAAGAGCGACGCTTGCTGCATTACTGAATAAGGGTTACTGA

Upstream 100 bases:

>100_bases
GGTTTCAGCCTAACATGTCTTCACCCGTTTTAAGCGCCAACAAACGACCGTTTTCCGGCATCCTCCAACCGCACGCTTTC
ACACTGTTGGAAAATTAGCC

Downstream 100 bases:

>100_bases
TATACTTAAAACATGCTCTAACACTTTGCAGATACGAGCAATACTTGGTAAAACAGCACACTTTCCGCCAGAATCGGATT
TTTGAGGTCCTGGCAACAGC

Product: resolvase TnpR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAFPAGHPIFITVFWQGPDEEMTMAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPAVQRKAFFEMLSKI
RDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDLTSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAK
AEGRKLGRPAKIAPEARGAILDKRAAGVSVSALAREYGVSRATLAALLNKGY

Sequences:

>Translated_212_residues
MAFPAGHPIFITVFWQGPDEEMTMAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPAVQRKAFFEMLSKI
RDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDLTSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAK
AEGRKLGRPAKIAPEARGAILDKRAAGVSVSALAREYGVSRATLAALLNKGY
>Mature_211_residues
AFPAGHPIFITVFWQGPDEEMTMAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPAVQRKAFFEMLSKIR
DGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDLTSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKA
EGRKLGRPAKIAPEARGAILDKRAAGVSVSALAREYGVSRATLAALLNKGY

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787827, Length=181, Percent_Identity=42.5414364640884, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1787638, Length=181, Percent_Identity=41.9889502762431, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787404, Length=179, Percent_Identity=37.4301675977654, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23238; Mature: 23107

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPAGHPIFITVFWQGPDEEMTMAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDV
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHH
VSGKVPAVQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQL
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCCCEEEEECC
GTTDLTSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCHHCCCCCCCCCHHHHHH
LDKRAAGVSVSALAREYGVSRATLAALLNKGY
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AFPAGHPIFITVFWQGPDEEMTMAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDV
CCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHH
VSGKVPAVQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQL
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCCCEEEEECC
GTTDLTSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCHHCCCCCCCCCHHHHHH
LDKRAAGVSVSALAREYGVSRATLAALLNKGY
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]