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The map label for this gene is gatA

Identifier: 209398381

GI number: 209398381

Start: 2844933

End: 2845385

Strand: Reverse

Name: gatA

Synonym: ECH74115_3073

Alternate gene names: 209398381

Gene position: 2845385-2844933 (Counterclockwise)

Preceding gene: 209400959

Following gene: 209399554

Centisome position: 51.07

GC content: 42.16

Gene sequence:

>453_bases
ATGACTAACCTGTTTGTTCGTAGCGGAATTTCTTTTGTCGATCGTAGCGAAGTTTTAACCCATATCGGTAATGAGATGCT
CGCCAAAGGTGTGGTTCATGATACCTGGCCACAGGCATTAATTGCCAGAGAAGCAGAATTCCCTACCGGGATAATGCTTG
AGCAGCACGCCATTGCAATACCGCATTGTGAGGCGATTCATGCTAAGTCGTCAGCCATTTATCTGTTAAGGCCAACAAAT
AAAGTTCATTTTCAGCAAGCGGATGATGATAATGACGTGGCGGTATCGTTGGTTATTGCGTTGATTGTGGAAAATCCGCA
GCAGCAATTGAAACTTTTACGCTGTTTATTTGGCAAGTTACAACAGCCCGATATCGTTGAGACACTAATCACTCTTCCTG
AAACTCAGTTAAAGGAATACTTCACAAAGTATGTTTTAGATTCAGACGAATAA

Upstream 100 bases:

>100_bases
GTCCGGGGAATTATCAGCAATACCGCATCAGCTGATTATGGATAAAATTTATGATGTTTTGCGCGCCTATCGCTACGGCT
GTGCGGAATAAGGACGGTAT

Downstream 100 bases:

>100_bases
ATCCCTCTGTAACAATAAAAAGGACTATTTATGAAACGCAAGATTATTGTCGCTTGCGGAGGCGCGGTTGCGACCTCTAC
GATGGCGGCGGAAGAAATTA

Product: PTS system galactitol-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gat; PTS system galactitol-specific EIIA component

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTN
KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE

Sequences:

>Translated_150_residues
MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTN
KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE
>Mature_149_residues
TNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNK
VHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1788410, Length=150, Percent_Identity=100, Blast_Score=311, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTKA_ECO57 (P69814)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90991
- PIR:   C85836
- RefSeq:   NP_288596.1
- RefSeq:   NP_310924.1
- ProteinModelPortal:   P69814
- SMR:   P69814
- EnsemblBacteria:   EBESCT00000028021
- EnsemblBacteria:   EBESCT00000055803
- GeneID:   916599
- GeneID:   956751
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3257
- KEGG:   ecs:ECs2897
- GeneTree:   EBGT00050000009664
- HOGENOM:   HBG467844
- OMA:   DESSYRI
- ProtClustDB:   PRK09665
- BioCyc:   ECOL83334:ECS2897-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- Gene3D:   G3DSA:3.40.930.10

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 16908; Mature: 16776

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAI
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEECCCEEEC
PHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKL
CCCHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
QQPDIVETLITLPETQLKEYFTKYVLDSDE
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAI
CCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEECCCEEEC
PHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKL
CCCHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
QQPDIVETLITLPETQLKEYFTKYVLDSDE
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796