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The map label for this gene is insO2 [H]

Identifier: 209398278

GI number: 209398278

Start: 4685913

End: 4686659

Strand: Direct

Name: insO2 [H]

Synonym: ECH74115_5034

Alternate gene names: 209398278

Gene position: 4685913-4686659 (Clockwise)

Preceding gene: 209397454

Following gene: 209399834

Centisome position: 84.1

GC content: 53.01

Gene sequence:

>747_bases
TTGTGCAGCGAAATACGCCGGGCGTGGAACCAAAGTAGGGGCTCTGCGGGGGCGCGCACGCTGGCTGAAATGCTGACCCA
AAACGGCGTCCCGATGAGCCGTTACCGTGCCGGGCGTCTGATGAAATATCTGAACCTGAGCAGTTGTCAGCCCGGAAAAC
ATCAGTACAAAAATGCTCGTCAGGAACATACCTGCCTGCCGAATCTGCTTGAGCGCCAGTTCGCTGTGCCCGAGCCAGAT
AGGGTATGGTGCGGAGATATTACGTATATCTGGGCAGGAAATCGCTGGTGCTATCTGGCGGTCGTCATGGATCTTTTTGC
CCGCAGGGTTATCGGCTGGAGCCTGTCAGCGAATGCCGATACTGCCCTGATAAGCAGTGCCCTGCGGATGGCGTATGAGG
TGCGTGGTCAGCCGCGGGACGTCATGTTCCATAGCGACCAGGGAAGTCAATATACAGGACTGAAATATCAACAACTTCTC
TGGCGTTACAGGATAAAGCAAAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCAATGGAACGCTTCTTCCGCAG
TCTGAAAACAGAATGGGTGCCAACCGATGGCTACACAGGCAAAGACGTGGCCCGGCAGCAAATCAGCAGTTATATTCTGA
ATTATTACAATAGCGTCAGGCCTCACCATTATAACGGAGGGCTGACGCCGGAAGAGTCAGAGAACAGATATCATTTTTAC
TGTAAAACCGTGGCCAGTATTACTTGA

Upstream 100 bases:

>100_bases
CCACTCAGTTGTCAGCCTCTGTTCTGCTCTGGAAATACACCGCAGCAGTTACCAGTACTGGCGAAAACGACGCGATACGG
TTAATCCGGCGCGAGTCAGG

Downstream 100 bases:

>100_bases
CCACTACACTGGAACTCATGCTCACCAGCGGTGAGCTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGG
CTGACCTGCAAAGCCGATAC

Product: IS911 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPD
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLL
WRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFY
CKTVASIT

Sequences:

>Translated_248_residues
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPD
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLL
WRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFY
CKTVASIT
>Mature_248_residues
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPD
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLL
WRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFY
CKTVASIT

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=210, Percent_Identity=32.3809523809524, Blast_Score=124, Evalue=7e-29,
Organism=Escherichia coli, GI1788405, Length=237, Percent_Identity=35.4430379746835, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787263, Length=237, Percent_Identity=35.4430379746835, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786753, Length=237, Percent_Identity=35.4430379746835, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786570, Length=237, Percent_Identity=35.4430379746835, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786490, Length=237, Percent_Identity=35.4430379746835, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789981, Length=233, Percent_Identity=34.3347639484979, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28851; Mature: 28851

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNAR
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
HHHHHHHHHHHHHCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCH
TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSP
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
MERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
CKTVASIT
EHHHHHCC
>Mature Secondary Structure
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNAR
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
HHHHHHHHHHHHHCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCH
TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSP
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
MERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
CKTVASIT
EHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]