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The map label for this gene is ulaC

Identifier: 209398074

GI number: 209398074

Start: 5350635

End: 5351099

Strand: Direct

Name: ulaC

Synonym: ECH74115_5711

Alternate gene names: 209398074

Gene position: 5350635-5351099 (Clockwise)

Preceding gene: 209397475

Following gene: 209396289

Centisome position: 96.03

GC content: 53.55

Gene sequence:

>465_bases
ATGAAATTACGTGATTCGCTGGCTGAAAATAAATCCATCCGTCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAA
AATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGT
TCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTC
TCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGC
GGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTT
TACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCAGCTTAA

Upstream 100 bases:

>100_bases
GGTTGGCGTGCGCAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGG
ACGTGAAGTAAGGACGCGCT

Downstream 100 bases:

>100_bases
GAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTC
GCCTGATTGCCGAAGAAGTC

Product: PTS system L-ascorbate-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGF
SLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA

Sequences:

>Translated_154_residues
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGF
SLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA
>Mature_154_residues
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGF
SLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI2367359, Length=154, Percent_Identity=100, Blast_Score=317, Evalue=3e-88,
Organism=Escherichia coli, GI1790755, Length=103, Percent_Identity=39.8058252427184, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1789302, Length=120, Percent_Identity=40, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790027, Length=142, Percent_Identity=33.8028169014084, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ULAC_ECO57 (P69821)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C86116
- PIR:   C91275
- RefSeq:   NP_290825.1
- RefSeq:   NP_313198.1
- ProteinModelPortal:   P69821
- SMR:   P69821
- EnsemblBacteria:   EBESCT00000028904
- EnsemblBacteria:   EBESCT00000055153
- GeneID:   914001
- GeneID:   959928
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5804
- KEGG:   ecs:ECs5171
- GeneTree:   EBGT00050000009676
- HOGENOM:   HBG697201
- OMA:   PEEGVIK
- ProtClustDB:   PRK10372
- BioCyc:   ECOL83334:ECS5171-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- Gene3D:   G3DSA:3.40.930.10

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 17238; Mature: 17238

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS00387 PPASE

Important sites: ACT_SITE 68-68

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
CCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEE
APGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQ
ECCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHH
IVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
CCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEE
APGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQ
ECCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHH
IVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796