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The map label for this gene is ligB [H]

Identifier: 209397991

GI number: 209397991

Start: 4666454

End: 4668187

Strand: Reverse

Name: ligB [H]

Synonym: ECH74115_5017

Alternate gene names: 209397991

Gene position: 4668187-4666454 (Counterclockwise)

Preceding gene: 209396688

Following gene: 209399724

Centisome position: 83.78

GC content: 52.83

Gene sequence:

>1734_bases
TTGCCTTCACCACGTTGTATATCTGCCAGCCTGACTACAGCGGAGGTGAAGATGAAAGTATGGATGGCGATATTAATAAG
TATCTTGTGCTGGCAATCATCTGTGTGGGCGGTCTGTCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTTCCGCC
TGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAG
TTAAGCGCCCGTCTTACGCAGTGGCAACGCTGCTTTGTGAGCGAGCCCCGCGACGTGATGATGCCGCCTTTAAATGGTGC
GGTTATGCATCCGGTTGCCCATACGGGTGTACGCAAAATGGCGGATAAAAACGCATTAAGTCTGTGGATGCGAGAACGTA
GCGATCTTTGGGTGCAGCCAAAAGTTGATGGCGTTGCGGTAACCCTGGTTTATCGGGACGGGAAACTGAACAAAGCAATC
AGTCGCGGTAATGGCCTGAAAGGCGAGGACTGGACGCAGAAAGTTAGCTTAATTTCCGCTGTGCCGCAAACCGTTAGCGG
GCCTTTAGCCAACAGTACGCTTCAGGGGGAAATATTTCTCCAGCGCGAGGGGCATATCCAACAACAAATGGGGGGAATAA
ATGCCCGCGCAAAAGTTGCTGGCCTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGG
GCATGGCCGGATGGACCGCAGTTAATGACCGATCGTTTAAAGGAGCTGGCTACCGCAGGTTTTACTCTAACGCAGAGGTA
TACCCGTGCGGTTAAAAATGCTGATGAGGTTGCGCGCGTACGCAATGAGTGGTGGAAAGCGAAATTACCCTTCGTCACCG
ATGGCGTGGTTGTGCGAGGGGCGAAAGAACCCGAATCCCGCCATTGGCTACCAGGCCAGGCAGAATGGCTGGTGGCCTGG
AAATATCAACCTGTAGCTCAGGTTGCCAAAGTGAAGGCGATTCAGTTTGCGGTGGGTAAGAGCGGTAAAATATCGGTGGT
TGCGTCACTCGCACCTGTCATGCTGGATGATAAAAAAGTCCAGCGGGTGAATATTGGTTCCGTCAGGCGCTGGCAGGAGT
GGGATATTGCGCCTGGTGATCAGATCCTCGTCAGCCTTGCCGGTCAGGGTATTCCTCGCATTGATGATGTGGTCTGGCGC
GGTGCAGAACGTACAAAACCGACACCACCAGAAAACCGCTTTAACTCGTTGACCTGCTACTTTGCTTCTGATGTTTGTCA
GGAACAGTTCATTTCACGCTTAGTCTGGCTGGGGTCAAAACAGGTTCTGGGGCTGGATGGCATTGGTGAGGCCGGCTGGC
GCGCGCTGCATCAGACTCATCGCTTTGAGCATATCTTTTCCTGGCTTTTATTAACGCCAGAGCAATTACAGAACACGCCG
GGGATCGCGAAAAGTAAAAGTGCGCAGCTATGGCATCGGTTTAATCTGGCTCGTAAGCAGCCTTTTACTCGCTGGGTGAT
GGCAATGGGAATACCGCTAACCCGGGCGGCGCTTAATGCCAGTGATGAACGGTCCTGGTCGCAACTTTTATTTAGCACGG
AGCAGTTCTGGCAGCAGCTGCCGGGGACTGGATCGGGGCGCGCCAGACAGGTTATTGAATGGAAGGAAAATGCGCAAATC
AAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGATCACAGGTTTTGAACCTTAG

Upstream 100 bases:

>100_bases
TTGCGTTTTGCGTAGCTGTTTCCGCATTTGCCTGTGATTTACTGCAATAAAGACGATTCTCAGGAAGCGTCCTCGCAGAC
TCCGTTTTTGCCACTCGGCT

Downstream 100 bases:

>100_bases
TGTCCTTCGTGGCTGTAGTAAAAGACCGGTAATCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATAC
CAGGGTGGAGATAATTACCA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MPSPRCISASLTTAEVKMKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQWDDDYWKEGKSEVEDGVYDQ
LSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAI
SRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVW
AWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAW
KYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWR
GAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTP
GIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQI
KKLGSWLAAQQITGFEP

Sequences:

>Translated_577_residues
MPSPRCISASLTTAEVKMKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQWDDDYWKEGKSEVEDGVYDQ
LSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAI
SRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVW
AWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAW
KYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWR
GAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTP
GIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQI
KKLGSWLAAQQITGFEP
>Mature_576_residues
PSPRCISASLTTAEVKMKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQWDDDYWKEGKSEVEDGVYDQL
SARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAIS
RGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWA
WPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWK
YQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRG
AERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPG
IAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIK
KLGSWLAAQQITGFEP

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=560, Percent_Identity=98.0357142857143, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=408, Percent_Identity=26.2254901960784, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 65154; Mature: 65023

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPRCISASLTTAEVKMKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQ
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
WDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKM
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEECHHHHHHHHHH
ADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISA
HHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHH
VPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVW
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEE
AWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRG
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEEC
AKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKV
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHCCCCHH
QRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCY
EEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEE
FASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTP
HHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PSPRCISASLTTAEVKMKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQ
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
WDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKM
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEECHHHHHHHHHH
ADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISA
HHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHH
VPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVW
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEE
AWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRG
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEEC
AKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKV
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHCCCCHH
QRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCY
EEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEE
FASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTP
HHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA