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The map label for this gene is ybiS

Identifier: 209397897

GI number: 209397897

Start: 982492

End: 983406

Strand: Reverse

Name: ybiS

Synonym: ECH74115_0968

Alternate gene names: 209397897

Gene position: 983406-982492 (Counterclockwise)

Preceding gene: 209397491

Following gene: 209398680

Centisome position: 17.65

GC content: 53.55

Gene sequence:

>915_bases
ATGAAATTGAAAACATTATTCGCAGCGGCCTTCGCTGTTGTCGGCTTTTGCAGTACCGCCTCTGCGGTAACTTATCCTCT
GCCAACCGACGGGAGTCGCCTGGTTGGTCAGAATCAGGTGATCACCATTCCTGAAGGTAACACTCAGCCGCTGGAGTATT
TTGCCGCGGAGTACCAGATGGGGCTTTCCAATATGATGGAAGCGAACCCGGGTGTGGATACCTTCCTGCCGAAAGGCGGT
ACTGTACTGAACATTCCGCAGCAGCTGATCCTGCCGGATACCGTTCATGAAGGCATCGTCATTAACAGTGCAGAGATGCG
TCTGTATTACTATCCGAAAGGGACCAACACCGTTATCGTGCTGCCGATCGGCATTGGTCAGTTAGGCAAAGATACGCCTA
TCAACTGGACCACCAAAGTTGAGCGTAAGAAAGCAGGCCCGACCTGGACGCCGACCGCCAAAATGCACGCAGAGTACCGC
GCTGCGGGCGAACCGCTTCCGGCTGTCGTTCCGGCAGGTCCGGATAACCCGATGGGGCTGTATGCACTCTACATCGGTCG
CCTGTACGCTATCCATGGCACCAACGCTAACTTCGGTATCGGTCTGCGTGTAAGTCATGGTTGTGTGCGTCTGCGTAACG
AAGACATCAAATTCCTGTTCGAGAAAGTACCGGTCGGTACCCGCGTACAGTTTATTGATGAGCCGGTAAAAGCGACCACC
GAGCCAGACGGCAGCCGTTACATTGAAGTCCATAACCCGCTGTCTACCACCGAAGCCCAATTTGAAGGTCAGGAAATTGT
GCCAATTACCCTGACGAAGAGCGTGCAGACAGTGACCGGCCAGCCAGATGTTGACCAGGTTGTTCTTGATGAAGCGATTA
AAAACCGCTCCGGGATGCCGGTTCGTCTGAATTAA

Upstream 100 bases:

>100_bases
GATTTCACTGTGAGCTGGAATGAACTTATAATGCGCTTCCAATACTCTAATATTCTCAACCCAATGGCCTGCCAGGCACA
AAATCTCGCTTAACATGAAT

Downstream 100 bases:

>100_bases
TCTTCAAAACGTAAAACAAAGGCGGACTCATAATCCGCCTTTTTTATTTGCCAGACCTTACCAGGCCGGGATCATAACCA
ACAAAACATATCCCACCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVITIPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGG
TVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYR
AAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEKVPVGTRVQFIDEPVKATT
EPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKSVQTVTGQPDVDQVVLDEAIKNRSGMPVRLN

Sequences:

>Translated_304_residues
MKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVITIPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGG
TVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYR
AAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEKVPVGTRVQFIDEPVKATT
EPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKSVQTVTGQPDVDQVVLDEAIKNRSGMPVRLN
>Mature_304_residues
MKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVITIPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGG
TVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYR
AAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEKVPVGTRVQFIDEPVKATT
EPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKSVQTVTGQPDVDQVVLDEAIKNRSGMPVRLN

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family

Homologues:

Organism=Escherichia coli, GI1787040, Length=304, Percent_Identity=100, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1788299, Length=296, Percent_Identity=64.1891891891892, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787356, Length=311, Percent_Identity=48.2315112540193, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1787968, Length=305, Percent_Identity=45.5737704918033, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YBIS_ECO57 (P0AAY0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85591
- PIR:   H90740
- RefSeq:   NP_286583.1
- RefSeq:   NP_308923.1
- ProteinModelPortal:   P0AAY0
- SMR:   P0AAY0
- EnsemblBacteria:   EBESCT00000026363
- EnsemblBacteria:   EBESCT00000058460
- GeneID:   917644
- GeneID:   958084
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1041
- KEGG:   ecs:ECs0896
- GeneTree:   EBGT00050000008974
- HOGENOM:   HBG295778
- OMA:   PPPGEDI
- ProtClustDB:   PRK10260
- BioCyc:   ECOL83334:ECS0896-MONOMER
- InterPro:   IPR005490

Pfam domain/function: PF03734 YkuD

EC number: NA

Molecular weight: Translated: 33080; Mature: 33080

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVITIPEGNTQPLEYFAAEYQM
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHHH
GLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIV
HHHHHHHCCCCCCEECCCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEEEE
LPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPMGL
EEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHH
YALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEKVPVGTRVQFIDEPVKATT
HHEEEEEEEEEECCCCCEEEEEEEECCEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCC
EPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKSVQTVTGQPDVDQVVLDEAIKNRSGMP
CCCCCEEEEECCCCCCCHHHCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCC
VRLN
EEEC
>Mature Secondary Structure
MKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVITIPEGNTQPLEYFAAEYQM
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHHH
GLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIV
HHHHHHHCCCCCCEECCCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEEEE
LPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPMGL
EEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHH
YALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEKVPVGTRVQFIDEPVKATT
HHEEEEEEEEEECCCCCEEEEEEEECCEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCC
EPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKSVQTVTGQPDVDQVVLDEAIKNRSGMP
CCCCCEEEEECCCCCCCHHHCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCC
VRLN
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796