Click here to switch to the map view.

The map label for this gene is rapA [H]

Identifier: 209397603

GI number: 209397603

Start: 65271

End: 68030

Strand: Reverse

Name: rapA [H]

Synonym: ECH74115_0064

Alternate gene names: 209397603

Gene position: 68030-65271 (Counterclockwise)

Preceding gene: 209398156

Following gene: 209396031

Centisome position: 1.22

GC content: 55.22

Gene sequence:

>2760_bases
GTGACCCGCGTGATGTTCAACCCTGGTGATACCATTACCAGCCATGACGGCTGGCAGATGCAAGTCGAAGAAGTAAAAGA
AGAAAATGGCTTGCTGACCTATATCGGTACTCGCCTGGATACTGAAGAGTCCGGCGTAGCCCTGCGTGAAGTTTTCCTTG
ATAGCAAACTGGTGTTCAGCAAACCGCAGGACCGCCTGTTTGCCGGGCAGATTGACCGTATGGACCGCTTTGCGCTGCGT
TATCGCGCGCGTAAGTATTCCAGCGAACAGTTCCGTATGCCGTACAGCGGCCTGCGCGGTCAGCGTACCAGCCTGATCCC
GCACCAGCTCAACATCGCTCATGATGTTGGCCGCCGCCACGCGCCGCGCGTCCTGCTGGCTGACGAAGTGGGTTTAGGGA
AAACCATTGAAGCCGGGATGATCCTTCATCAGCAACTGCTCTCTGGCGCTGCTGAACGTGTGCTGATTATCGTCCCGGAA
ACCTTACAGCATCAGTGGCTGGTAGAAATGCTGCGCCGTTTCAACCTGCGCTTTGCGCTGTTTGATGATGAGCGTTATGC
CGAAGCTCAGCACGATGCTTACAACCCGTTCGACACCGAACAGCTGGTGATTTGCTCGCTGGATTTTGCCCGCCGTAGCA
AACAGCGTCTGGAACATCTCTGTGAAGCCGAGTGGGACCTGCTGGTGGTCGATGAAGCGCATCACCTGGTGTGGAGCGAA
GACGCGCCGAGCCGCGAATATCAGGCTATTGAACAACTGGCAGAGCACGTGCCGGGCGTTCTGCTGCTGACCGCAACCCC
GGAACAGCTGGGAATGGAAAGCCACTTCGCCCGTCTGCGTCTGCTGGACCCGAACCGTTTCCACGATTTCGCGCAATTCG
TTGAAGAGCAGAAAAATTATCGTCCAGTAGCGGACGCCGTTGCCATGCTGCTGGCAGGTAACAAACTGAGCAATGACGAA
CTGAACATGCTTGGCGAGATGATCGGCGAGCAGGATATCGAGCCGCTGTTGCAAGCAGCAAACAGCGACAGCGAAGATGC
CCAGAGCGCCCGTCAGGAGCTGGTTTCGATGCTGATGGATCGCCACGGCACCAGCCGCGTGCTGTTCCGTAACACCCGTA
ACGGTGTGAAAGGCTTCCCGAAACGCGAGCTGCACACCATTAAGCTGCCGCTACCGACGCAGTATCAGACGGCTATTAAA
GTCTCCGGCATTATGGGCGCACGTAAAAGTGCGGAAGACCGCGCCCGCGATATGCTCTACCCGGAGCGTATTTATCAGGA
ATTTGAAGGTGATAACGCCACCTGGTGGAACTTCGATCCGCGCGTTGAGTGGCTGATGGGCTACCTGACCAGCCATCGCT
CTCAGAAAGTGCTGGTGATCTGCGCTAAAGCTGCCACTGCGCTGCAACTGGAGCAAGTACTGCGCGAACGTGAAGGTATT
CGCGCTGCGGTGTTCCACGAAGGTATGTCGATTATCGAACGTGACCGCGCTGCCGCCTGGTTTGCCGAAGAAGACACCGG
CGCACAGGTACTGCTGTGCTCAGAAATCGGTTCTGAAGGACGTAACTTCCAGTTCGCCAGCCACATGGTGATGTTTGACC
TGCCATTCAACCCGGATCTACTGGAGCAGCGTATTGGTCGTCTGGATCGTATCGGCCAAGCGCACGATATTCAGATCCAT
GTGCCTTATCTGGAGAAAACCGCTCAGTCGGTGCTGGTGCGCTGGTATCACGAAGGTCTGGATGCATTTGAGCACACCTG
CCCGACCGGACGCACTATTTACGATAGCGTATACAACGATCTGATTAACTATCTGGCTTCACCGGATCAAACCGAAGGCT
TTGACGATCTGATCAAAAACTGCCGCGAGCAACATGAAGCGCTGAAAGCACAGCTGGAACAGGGTCGTGACCGCCTGCTG
GAAATCCACTCCAACGGTGGCGAAAAAGCCCAGGCACTGGCAGAAAGCATTGAAGAGCAGGATGACGATACCAACCTGAT
CGCCTTCGCCATGAACCTGTTCGATATTATCGGTATCAATCAGGACGATCGCGGCGACAACATGATCGTGCTGACGCCGT
CCGATCATATGCTGGTGCCGGACTTCCCTGGCCTGTCGGAAGATGGCATCACCATCACCTTTGATCGTGAAGTGGCGCTG
GCGCGTGAAGATGCGCAGTTTATTACCTGGGAACATCCGCTGATCCGCAACGGTCTGGATCTGATTCTTTCTGGCGATAC
CGGTAGCAGCACGATTTCACTGTTAAAAAACAAAGCGTTGCCGGTAGGTACGCTGTTGGTGGAACTGATTTATGTGGTTG
AAGCCCAGGCTCCGAAGCAGTTGCAGCTCAACCGCTTCCTGCCACCGACGCCGGTACGTATGCTGCTGGATAAAAACGGC
AACAACCTGGCGGCGCAGGTAGAGTTTGAAACCTTTAACCGCCAGCTTAACGCGGTTAACCGTCACACCGGCAGCAAACT
GGTTAACGCCGTGCAGCAGGATGTTCACGCTATCCTTCAACTGGGTGAAGCGCAGATCGAGAAATCTGCCCGTGCATTGA
TTGATGCAGCACGTAACGAAGCCGACGAAAAACTGTCTGCCGAGCTGTCTCGTCTGGAAGCTCTGCGTGCAGTGAACCCG
AACATTCGTGACGACGAACTGACCGCCATTGAGAGCAACCGTCAGCAGGTAATGGAAAGCCTGGATCAGGCAGGTTGGCG
TCTGGATGCCCTGCGTTTGATCGTTGTAACGCATCAGTAA

Upstream 100 bases:

>100_bases
ATTGGGACTTGGAACCGTTGTCGCGGTGGATGCGCGAACTGTCACTTTACTTTTCCCATCTACTGGTGAAAACCGTCTGT
ACGCACGCAGTGATTCCCCC

Downstream 100 bases:

>100_bases
CGGAGCCGAAAATGGGGATGGAAAACTACAATCCACCGCAGGAACCCTGGTTGGTTATCCTGTATCAGGATGACCATATT
ATGGTGGTCAACAAGCCGAG

Product: ATP-dependent helicase HepA

Products: NA

Alternate protein names: ATP-dependent helicase hepA [H]

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:

>919_residues
MTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFLDSKLVFSKPQDRLFAGQIDRMDRFALR
YRARKYSSEQFRMPYSGLRGQRTSLIPHQLNIAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPE
TLQHQWLVEMLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDLLVVDEAHHLVWSE
DAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDE
LNMLGEMIGEQDIEPLLQAANSDSEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIK
VSGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLVICAKAATALQLEQVLREREGI
RAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIH
VPYLEKTAQSVLVRWYHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLEQGRDRLL
EIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVLTPSDHMLVPDFPGLSEDGITITFDREVAL
AREDAQFITWEHPLIRNGLDLILSGDTGSSTISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNG
NNLAAQVEFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKLSAELSRLEALRAVNP
NIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ

Sequences:

>Translated_919_residues
MTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFLDSKLVFSKPQDRLFAGQIDRMDRFALR
YRARKYSSEQFRMPYSGLRGQRTSLIPHQLNIAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPE
TLQHQWLVEMLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDLLVVDEAHHLVWSE
DAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDE
LNMLGEMIGEQDIEPLLQAANSDSEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIK
VSGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLVICAKAATALQLEQVLREREGI
RAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIH
VPYLEKTAQSVLVRWYHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLEQGRDRLL
EIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVLTPSDHMLVPDFPGLSEDGITITFDREVAL
AREDAQFITWEHPLIRNGLDLILSGDTGSSTISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNG
NNLAAQVEFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKLSAELSRLEALRAVNP
NIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ
>Mature_918_residues
TRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFLDSKLVFSKPQDRLFAGQIDRMDRFALRY
RARKYSSEQFRMPYSGLRGQRTSLIPHQLNIAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPET
LQHQWLVEMLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDLLVVDEAHHLVWSED
APSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDEL
NMLGEMIGEQDIEPLLQAANSDSEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLVICAKAATALQLEQVLREREGIR
AAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHV
PYLEKTAQSVLVRWYHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLEQGRDRLLE
IHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVLTPSDHMLVPDFPGLSEDGITITFDREVALA
REDAQFITWEHPLIRNGLDLILSGDTGSSTISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGN
NLAAQVEFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKLSAELSRLEALRAVNPN
IRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ

Specific function: Transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on

COG id: COG0553

COG function: function code KL; Superfamily II DNA/RNA helicases, SNF2 family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1786245, Length=919, Percent_Identity=99.8911860718172, Blast_Score=1889, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR022737
- InterPro:   IPR000330 [H]

Pfam domain/function: PF00271 Helicase_C; PF12137 RapA_C; PF00176 SNF2_N [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 104465; Mature: 104333

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFLDSKLVFS
CCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEC
KPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLNIAHDVGRRH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCC
APRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVEMLRRFNLRFAL
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEE
FDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDLLVVDEAHHLVWSE
ECCHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECC
DAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQKNY
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHEEEECCCHHHHHHHHHHHHHHCC
RPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSDSEDAQSARQELVSMLMD
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKVSGIMGARKSAEDRARDMLY
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHEEHHHHCCCCCHHHHHHHCCC
PERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLVICAKAATALQLEQVLREREGI
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCC
RAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASHMVMFDLPFNPDL
EEHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCEEEECEEEEEECCCCHHH
LEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRWYHEGLDAFEHTCPTGRTIYDSVYND
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LINYLASPDQTEGFDDLIKNCREQHEALKAQLEQGRDRLLEIHSNGGEKAQALAESIEEQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHC
DDDTNLIAFAMNLFDIIGINQDDRGDNMIVLTPSDHMLVPDFPGLSEDGITITFDREVAL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCCCEEEEECCCEEE
AREDAQFITWEHPLIRNGLDLILSGDTGSSTISLLKNKALPVGTLLVELIYVVEAQAPKQ
ECCCCCEEEECCHHHHCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LQLNRFLPPTPVRMLLDKNGNNLAAQVEFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQ
CHHCCCCCCCCHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEAQIEKSARALIDAARNEADEKLSAELSRLEALRAVNPNIRDDELTAIESNRQQVMES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDQAGWRLDALRLIVVTHQ
HHHCCCEEEEEEEEEEECC
>Mature Secondary Structure 
TRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFLDSKLVFS
CEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEC
KPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLNIAHDVGRRH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCC
APRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVEMLRRFNLRFAL
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEE
FDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDLLVVDEAHHLVWSE
ECCHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECC
DAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQKNY
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHEEEECCCHHHHHHHHHHHHHHCC
RPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSDSEDAQSARQELVSMLMD
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKVSGIMGARKSAEDRARDMLY
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHEEHHHHCCCCCHHHHHHHCCC
PERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLVICAKAATALQLEQVLREREGI
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCC
RAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASHMVMFDLPFNPDL
EEHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCEEEECEEEEEECCCCHHH
LEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRWYHEGLDAFEHTCPTGRTIYDSVYND
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LINYLASPDQTEGFDDLIKNCREQHEALKAQLEQGRDRLLEIHSNGGEKAQALAESIEEQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHC
DDDTNLIAFAMNLFDIIGINQDDRGDNMIVLTPSDHMLVPDFPGLSEDGITITFDREVAL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCCCEEEEECCCEEE
AREDAQFITWEHPLIRNGLDLILSGDTGSSTISLLKNKALPVGTLLVELIYVVEAQAPKQ
ECCCCCEEEECCHHHHCCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LQLNRFLPPTPVRMLLDKNGNNLAAQVEFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQ
CHHCCCCCCCCHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEAQIEKSARALIDAARNEADEKLSAELSRLEALRAVNPNIRDDELTAIESNRQQVMES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDQAGWRLDALRLIVVTHQ
HHHCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA