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The map label for this gene is trmA [H]

Identifier: 209397508

GI number: 209397508

Start: 5074548

End: 5075648

Strand: Reverse

Name: trmA [H]

Synonym: ECH74115_5428

Alternate gene names: 209397508

Gene position: 5075648-5074548 (Counterclockwise)

Preceding gene: 209396990

Following gene: 209400281

Centisome position: 91.09

GC content: 51.04

Gene sequence:

>1101_bases
ATGACCCCCGAACACCTTCCAACAGAACAGTATGAAGCGCAGTTAGCCGAAAAAGTGGTACGTTTGCAAAGTATGATGGC
ACCGTTTTCTGACCTGGTTCCGGAAGTGTTTCGCTCGCCGGTCAGTCATTACCGGATGCGTGCGGAGTTCCGCATCTGGC
ACGATGGCGATGACCTGTACCACATCATTTTCGATCAACAAACCAAAAGCCGTATCCGGGTGGATAGCTTCCCCGCCGCC
AGTGAACTCATCAACCAGTTGATGACGGCGATGATTGCGGGTGTGCGTAATAATCCCGTTCTGCGCCACAAGTTGTTCCA
GATTGATTACCTCACCACGCTGAGTAATCAGGCGGTGGTTTCCCTGCTGTACCATAAAAAGCTGGATGATGAGTGGCGTC
AGGAAGCGGAAGCCCTGCGCGATGCACTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAA
ATCGCGCTGGATCAAGATTACATCGACGAGCGTCTGCCGGTCGCAGGGAAAGAGATGATCTATCGTCAGGTAGAAAACAG
CTTTACCCAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATT
TACTGGAGCTGTACTGCGGCAACGGTAACTTCTCATTAGCGCTGGCGCGCAATTTTGATCGGGTATTAGCCACCGAAATC
GCCAAGCCATCAGTCGCTGCCGCGCAATACAACATTGCCGCTAACCATATTGATAACGTACAAATTATTCGTATGGCGGC
AGAAGAATTTACTCAGGCGATGAATGGCGTGCGCGAGTTTAACCGCCTGCAAGGGATCGACTTAAAGAGTTATCAGTGCG
AAACCATTTTTGTCGACCCACCTCGCAGCGGTCTGGACAGTGAAACCGAGAAAATGGTGCAGGCGTATCCACGTATTCTG
TATATCTCCTGCAATCCGGAAACGTTATGCAAAAATCTGGAAACATTAAGCCAGACGCACAAGGTCGAACGTCTGGCTCT
GTTTGATCAGTTCCCCTACACGCACCATATGGAGTGCAGCGTATTACTGACCGCGAAGTAA

Upstream 100 bases:

>100_bases
ACTGTCCAGACGTAGCTCACAAATAGGAATTCATCAAGATCTGGACATCTGATGAGCAATCCCTACAATCGCCGCGTACT
TTAATTTTTCAGGATACATC

Downstream 100 bases:

>100_bases
AACCCATGCCGGATGCGCCAGCATCCGGCATGATACCGATTACTCAGCAACTTCCTGCTTACGGTTACGCATCTTCGCGC
CAATCCAGAACACCATAATG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECSVLLTAK

Sequences:

>Translated_366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECSVLLTAK
>Mature_365_residues
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAAS
ELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKI
ALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEIA
KPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILY
ISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECSVLLTAK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=99.4535519125683, Blast_Score=763, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=183, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=194, Percent_Identity=29.3814432989691, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 41939; Mature: 41808

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIALDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECS
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VLLTAK
EEEEEC
>Mature Secondary Structure 
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIALDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECS
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VLLTAK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA