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The map label for this gene is leuC [H]

Identifier: 209397303

GI number: 209397303

Start: 84052

End: 85452

Strand: Reverse

Name: leuC [H]

Synonym: ECH74115_0079

Alternate gene names: 209397303

Gene position: 85452-84052 (Counterclockwise)

Preceding gene: 209399607

Following gene: 209397741

Centisome position: 1.53

GC content: 55.6

Gene sequence:

>1401_bases
ATGGCTAAGACGTTATACGAAAAATTATTCGACGCTCACGTAGTGTACGAAGCCGAAAATGAAACCCCGCTGTTATATAT
CGACCGCCACCTGGTGCATGAAGTGACCTCACCGCAGGCGTTCGATGGTCTGCGCGCCCACGGTCGCCCGGTACGTCAGC
CGGGCAAAACCTTCGCCACCATGGATCACAACGTCTCTACCCAGACTAAAGACATTAATGCCTGCGGTGAAATGGCGCGC
ATCCAGATGCAGGAGCTGATCAAAAACTGCAAAGAATTTGGCGTCGAACTGTATGACCTGAATCACCCCTATCAAGGGAT
CGTCCACGTAATGGGGCCGGAACAGGGCGTGACCTTGCCGGGGATGACCATTGTCTGCGGCGACTCGCATACCGCCACCC
ACGGCGCGTTTGGCGCACTGGCCTTTGGTATCGGCACTTCCGAAGTTGAACACGTACTGGCAACGCAAACCCTGAAACAG
GGCCGTGCGAAGACCATGAAAATTGAAGTCCAGGGCAAAGCCGCGCCGGGCATTACCGCAAAAGATATCGTGTTGGCAAT
TATCGGTAAAACCGGTAGCGCAGGCGGCACCGGGCATGTGGTGGAGTTTTGCGGCGAAGCAATCCGTGATTTAAGCATGG
AAGGTCGGATGACCCTGTGCAATATGGCAATCGAAATGGGCGCGAAAGCCGGTCTGGTTGCACCTGACGAAACCACCTTT
AACTATGTCAAAGGCCGTCTGCATGCGCCGAAAGGCAAAGATTTCGACGACGCCGTTGCCTACTGGAAAACCCTGCAAAC
CGACGAAGGCGCAACTTTCGATACCGTTGTCACTTTGCAGGCAGAAGAGATTTCACCGCAGGTCACCTGGGGAACTAACC
CAGGCCAGGTGATTTCCGTGAACGACAATATTCCCGATCCGGCTTCGTTTGCCGATCCGGTTGAACGCGCGTCGGCAGAA
AAAGCGTTGGCCTATATGGGGCTGAAACCGGGTATTCTGCTGACCGAAGTGGCTATCGACAAAGTGTTTATCGGTTCCTG
CACCAACTCACGTATTGAAGATTTACGCGCGGCGGCGGAAATCGCCAAAGGGCGGAAAGTCGCGCCAGGCGTACAGGCGC
TGGTGGTTCCCGGCTCTGGTCCGGTAAAAGCGCAGGCGGAAGCAGAAGGTCTGGATAAAATCTTTATTGAAGCCGGTTTT
GAATGGCGCTTGCCTGGCTGCTCAATGTGTCTGGCGATGAACAACGACCGTCTGAATCCGGGCGAACGTTGTGCCTCCAC
CAGCAACCGTAACTTTGAAGGCCGTCAGGGGCGCGGCGGGCGCACGCATCTGGTCAGCCCGGCAATGGCTGCCGCCGCGG
CTGTGACCGGCCATTTCGCCGACATTCGCAACATTAAATAA

Upstream 100 bases:

>100_bases
AAGGCATTCGCACCGGGGATTTAGCCCGTGGCGCTGCCGCCGTTAGTACCGATGAAATGGGCGATATCATTGCCCGCTAT
GTGGCAGAAGGGGTGTAATC

Downstream 100 bases:

>100_bases
GGAGCACACCATGGCAGAGAAATTTATCAAACACACAGGCCTGGTGGTTCCGCTGGATGCCGCCAATGTCGATACCGATG
CAATCATCCCGAAACAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMAR
IQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAE
KALAYMGLKPGILLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK

Sequences:

>Translated_466_residues
MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMAR
IQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAE
KALAYMGLKPGILLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK
>Mature_465_residues
AKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARI
QMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQG
RAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFN
YVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEK
ALAYMGLKPGILLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFE
WRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=26.5151515151515, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4501867, Length=413, Percent_Identity=26.1501210653753, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=26.2337662337662, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=99.7854077253219, Blast_Score=962, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=26.7409470752089, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367097, Length=363, Percent_Identity=26.4462809917355, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=475, Percent_Identity=27.1578947368421, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=27.9805352798054, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=26.9736842105263, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.5095541401274, Blast_Score=584, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320440, Length=417, Percent_Identity=24.7002398081535, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=409, Percent_Identity=28.6063569682152, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=388, Percent_Identity=27.5773195876289, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=476, Percent_Identity=24.3697478991597, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864292, Length=476, Percent_Identity=24.3697478991597, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=24.5742092457421, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=411, Percent_Identity=26.0340632603406, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=27.3712737127371, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137564, Length=455, Percent_Identity=25.4945054945055, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49898; Mature: 49767

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEEEE
MDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEE
NDNIPDPASFADPVERASAEKALAYMGLKPGILLTEVAIDKVFIGSCTNSRIEDLRAAAE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHEEEECCCCHHHHHHHHHHH
IAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEEEE
MDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEE
NDNIPDPASFADPVERASAEKALAYMGLKPGILLTEVAIDKVFIGSCTNSRIEDLRAAAE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHEEEECCCCHHHHHHHHHHH
IAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA