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The map label for this gene is uhpB [H]

Identifier: 209397016

GI number: 209397016

Start: 4738870

End: 4740375

Strand: Reverse

Name: uhpB [H]

Synonym: ECH74115_5098

Alternate gene names: 209397016

Gene position: 4740375-4738870 (Counterclockwise)

Preceding gene: 209398144

Following gene: 209396606

Centisome position: 85.07

GC content: 57.3

Gene sequence:

>1506_bases
GTGATGAAGACGTTGTTCTCCCGCTTAATTACCGTTATTGCCTGCTTTTTTATCTTCTCTGCCGCATGGTTTTGCCTGTG
GAGTATCAGCCTGCACCTGGTTGAGCGCCCTGATATGGCGGTGCTGTTATTTCCGTTTGGTCTGCGGCTAGGGCTAATGC
TGCAATGCCCGCGCGGATACTGGCCGGTGCTGCTGGGAGCGGAGTGGCTGCTGATTTACTGGCTAACGCAGGCGGTCGGT
TTAACCCATTTCTCCTTATTGATGATCGGTAGTTTACTGACGTTACTGCCCGTGGCGCTGATCTCGCGCTATCGCCATCA
ACGTGACTGGCGCACCTTGCTGTTACAGGGTGCGGCGTTAACGGCGGCGGCGTTGTTGCAGTCGCTGCCCTGGCTTTGGC
ACGGCAAAGAGTCGTGGAATGCGCTGCTGTTAACTTTAACTGGCGGCCTGACGCTGGCCCCGATATGTCTGGTGTTCTGG
CATTATCTCGCCAATAACACCTGGCTGCCGCTCGGGCCGTCGTTAGTTTCTCAACCGATCAACTGGCGCGGGCGGCATCT
GGTCTGGTACTTGCTGCTGTTTGTTATCAGTCTCTGGCTCCAGTTGGGCTTGCCGGACGAACTGTCGCGCTTTACGCCAT
TCTGCCTGGCGCTGCCGATTATCGCGCTGGCCTGGCACTACGGCTGGCAAGGGGCGCTGATAGCGACATTGATGAACGCC
ATCGCGCTGATCGCCAGTCAAACCTGGCGCGATCATCCTGTGGATTTATTGCTCTCGCTGCTGGTGCAAAGTCTGACAGG
GTTGTTACTGGGCGCTGGCATCCAGCGGTTGCGTGAACTTAACCAGTCGCTGCAAAAGGAACTGGCGCGCAATCAGCATC
TCGCTGAACGTTTGTTAGAAACTGAAGAGAGCGTGCGCCGTGATGTGGCGCGTGAGCTGCATGATGATATCGGCCAGACC
ATCACTGCCATTCGTACTCAAGCGGGCATTGTCCAGCGACTGGCGGCAGATAACGCCAGTGTGAAGCAGAGCGGGCAGCT
CATCGAACAACTGTCGCTGGGCGTGTACGACGCGGTGCGCCGTTTGTTAGGGCGGTTACGTCCGCGCCAGTTGGATGATC
TCACCCTAGAGCAGGCCATCCGCTCACTGATGCGGGAAATGGAGCTGGAAGGGCGCGGCATTGTCAGCCATCTCGAATGG
CGAATCGAAGAGTCAGCGTTAAGCGAAAACCAGCGCGTGACGCTGTTTCGTGTCTGCCAGGAAGGGCTGAATAACATTGT
GAAACATGCTGATGCCAGCGCGGTCACCCTGCAAGGCTGGCTGCAGGATGAACGGTTGATGCTGGTGATTGAAGACGATG
GCAGCGGTTTACCGCCGGGTTCCGGGCAACAAGGTTTTGGCCTCACCGGAATGCGCGAGCGCGTAACGGCGCTGGGCGGC
ACCTTGACCATTTCCTGTCTGCACGGCACGCGTGTCAGCGTTTCTCTACCTCAACGCTATGTCTAA

Upstream 100 bases:

>100_bases
GCTTGTCACCGAAAACGGTACACGTCCATCGCGCCAATCTGATGGAAAAACTGGGCGTCAGTAACGACGTTGAACTGGCG
CGCCGTATGTTTGATGGCTG

Downstream 100 bases:

>100_bases
GGTTTGATGATGTTGCCGTTTCTGAAAGCGCCTGCCGATGCGCCATTAATGACTGATAAACATGAAATTGATGCCCGTTA
TCGCTACTGGCGTCGGCATA

Product: sensory histidine kinase UhpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MMKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVG
LTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFW
HYLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
IALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQT
ITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEW
RIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGG
TLTISCLHGTRVSVSLPQRYV

Sequences:

>Translated_501_residues
MMKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVG
LTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFW
HYLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
IALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQT
ITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEW
RIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGG
TLTISCLHGTRVSVSLPQRYV
>Mature_501_residues
MMKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVG
LTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFW
HYLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
IALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQT
ITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEW
RIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGG
TLTISCLHGTRVSVSLPQRYV

Specific function: Member of the two-component regulatory system uhpB/uhpA involved in the regulation of the uptake of hexose phosphates. UhpB functions as a membrane-associated protein kinase that phosphorylates uhpA in response to environmental signals [H]

COG id: COG3851

COG function: function code T; Signal transduction histidine kinase, glucose-6-phosphate specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=500, Percent_Identity=99.2, Blast_Score=984, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=214, Percent_Identity=33.1775700934579, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787474, Length=219, Percent_Identity=26.9406392694064, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR007895
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3; PF05231 MASE1 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56391; Mature: 56391

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHEECCCCC
WPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWHYLANNTWLPLGPSLVSQPI
HHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
NWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEESVRRDVARELHDDIGQTITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
RLLGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEWRIEESALSENQRVTLFRVCQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGLNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGG
HHHHHHHHHCCCCCEEHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCC
TLTISCLHGTRVSVSLPQRYV
CEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure
MMKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHEECCCCC
WPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWHYLANNTWLPLGPSLVSQPI
HHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
NWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEESVRRDVARELHDDIGQTITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
RLLGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEWRIEESALSENQRVTLFRVCQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGLNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGG
HHHHHHHHHCCCCCEEHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCC
TLTISCLHGTRVSVSLPQRYV
CEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3301805; 1569007; 7686882; 9278503 [H]