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The map label for this gene is iraD [H]

Identifier: 209396977

GI number: 209396977

Start: 5485463

End: 5485855

Strand: Direct

Name: iraD [H]

Synonym: ECH74115_5835

Alternate gene names: 209396977

Gene position: 5485463-5485855 (Clockwise)

Preceding gene: 209398073

Following gene: 209396723

Centisome position: 98.45

GC content: 48.35

Gene sequence:

>393_bases
ATGATGCGCCAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATTGCTGCGTAAATCGGTATGCAG
CGATCTCCTCACTCTTTTTAATTCTCCTCATTCGACATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGC
ATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATT
GCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGA
TGAACCGCTAACGTTTGATCTGGTCTGGGATAATGGCGGTTGGCGCAGTGCGACGCTTGAGAATGTCAGCTAA

Upstream 100 bases:

>100_bases
ATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCT
AAACGTCAGGAGTGCGCAAA

Downstream 100 bases:

>100_bases
GCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACTGGATTCATGCGGGTATTCATCCGGTATGCGTAG
GCCTGAATCGGGATCACCAG

Product: DNA replication/recombination/repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRI
AALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS

Sequences:

>Translated_130_residues
MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRI
AALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS
>Mature_130_residues
MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRI
AALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS

Specific function: Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS during oxidative stress. Its effect on RpoS stability is due to its interaction with RssB, which probably blocks the interaction of Rss

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GpW/Gp25 family. IraD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082422, Length=130, Percent_Identity=98.4615384615385, Blast_Score=265, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007048 [H]

Pfam domain/function: PF04965 GPW_gp25 [H]

EC number: NA

Molecular weight: Translated: 14803; Mature: 14803

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLLVSGMPEWQVHNPSDKH
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHH
LQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGG
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHEEEEEEEEEECCCCEEEEEEECCCC
WRSATLENVS
CCCCCCCCCC
>Mature Secondary Structure
MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLLVSGMPEWQVHNPSDKH
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHH
LQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGG
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHEEEEEEEEEECCCCEEEEEEECCCC
WRSATLENVS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA