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The map label for this gene is fklB

Identifier: 209396918

GI number: 209396918

Start: 5359609

End: 5360229

Strand: Direct

Name: fklB

Synonym: ECH74115_5724

Alternate gene names: 209396918

Gene position: 5359609-5360229 (Clockwise)

Preceding gene: 209397707

Following gene: 209399441

Centisome position: 96.19

GC content: 55.56

Gene sequence:

>621_bases
ATGACCACCCCAACTTTTGACACCATCGAAGCGCAAGCAAGCTACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGA
ATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTC
CGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATG
GCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCG
CGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACG
GCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTTCCGGTTAATGGCGTGATCCCTGGCTGGATTGAA
GCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGG
CGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAA

Upstream 100 bases:

>100_bases
TTTTCCCTGGGATTTGTCATCATTCCCGCGACAAAGTTTTACCCAAAGAAGTGTGGCTGATATGCTGCCCGCTACTTTAT
ACCCTAAGAAAGGAAATACG

Downstream 100 bases:

>100_bases
GCAGCGCATTCTGTTCCCCTCGAACCAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATT
TTGTGTTGCAAAGACCCCGT

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: FKBP22; PPIase; Rotamase

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAM
AAEGVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL

Sequences:

>Translated_206_residues
MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAM
AAEGVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL
>Mature_205_residues
TTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMA
AEGVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIEA
LTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

COG id: COG0545

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 1

Gene ontology:

Cell location: Cytoplasm. Periplasm. Note=According to PubMed:7610040 it is periplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Homo sapiens, GI206725530, Length=114, Percent_Identity=47.3684210526316, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI17149844, Length=114, Percent_Identity=47.3684210526316, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI17149842, Length=114, Percent_Identity=47.3684210526316, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI17149836, Length=106, Percent_Identity=45.2830188679245, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4503725, Length=106, Percent_Identity=45.2830188679245, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4503729, Length=137, Percent_Identity=40.8759124087591, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4503727, Length=109, Percent_Identity=42.2018348623853, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4758380, Length=106, Percent_Identity=38.6792452830189, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI33469985, Length=88, Percent_Identity=45.4545454545455, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI192448443, Length=87, Percent_Identity=42.5287356321839, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI7706131, Length=88, Percent_Identity=39.7727272727273, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082390, Length=206, Percent_Identity=100, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789745, Length=210, Percent_Identity=37.1428571428571, Blast_Score=128, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540882, Length=106, Percent_Identity=45.2830188679245, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71993519, Length=106, Percent_Identity=39.622641509434, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17561782, Length=100, Percent_Identity=40, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320727, Length=88, Percent_Identity=53.4090909090909, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324194, Length=108, Percent_Identity=47.2222222222222, Blast_Score=85, Evalue=8e-18,
Organism=Drosophila melanogaster, GI281363946, Length=88, Percent_Identity=50, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17352457, Length=88, Percent_Identity=50, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24655568, Length=106, Percent_Identity=44.3396226415094, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583150, Length=108, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24647110, Length=100, Percent_Identity=41, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356629, Length=88, Percent_Identity=45.4545454545455, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): FKBB_ECOLI (P0A9L3)

Other databases:

- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_004707.1
- RefSeq:   NP_418628.4
- ProteinModelPortal:   P0A9L3
- SMR:   P0A9L3
- DIP:   DIP-31854N
- IntAct:   P0A9L3
- MINT:   MINT-1238607
- STRING:   P0A9L3
- SWISS-2DPAGE:   P0A9L3
- EnsemblBacteria:   EBESCT00000004635
- EnsemblBacteria:   EBESCT00000017107
- GeneID:   948726
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5746
- KEGG:   eco:b4207
- EchoBASE:   EB2396
- EcoGene:   EG12503
- eggNOG:   COG0545
- GeneTree:   EBGT00050000010913
- HOGENOM:   HBG731200
- OMA:   PYSALVF
- ProtClustDB:   PRK11570
- BioCyc:   EcoCyc:G7865-MONOMER
- BioCyc:   MetaCyc:G7865-MONOMER
- Genevestigator:   P0A9L3
- GO:   GO:0005829
- InterPro:   IPR001179
- InterPro:   IPR000774
- Gene3D:   G3DSA:1.10.287.460
- PANTHER:   PTHR10516

Pfam domain/function: PF00254 FKBP_C; PF01346 FKBP_N

EC number: =5.2.1.8

Molecular weight: Translated: 22216; Mature: 22085

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHR
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
ALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPART
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
DRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIEALTLMPVGSKWELTIPQELA
CEEEEEEECEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHH
YGERGAGASIPPFSTLVFEVELLEIL
CCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHR
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
ALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPART
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
DRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIEALTLMPVGSKWELTIPQELA
CEEEEEEECEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHH
YGERGAGASIPPFSTLVFEVELLEIL
CCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8703024; 9298646