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The map label for this gene is yscN [H]

Identifier: 209396453

GI number: 209396453

Start: 4708798

End: 4710138

Strand: Reverse

Name: yscN [H]

Synonym: ECH74115_5062

Alternate gene names: 209396453

Gene position: 4710138-4708798 (Counterclockwise)

Preceding gene: 209395993

Following gene: 209400182

Centisome position: 84.53

GC content: 44.37

Gene sequence:

>1341_bases
ATGATTTCAGAGCATGATTCTGTATTGGAAAAATACCCACGTATTCAGAAAGTGCTCAATAGCACTGTGCCGGCATTATC
ATTAAATTCGTCTACCAGATATGAAGGCAAGATTATCAATATCGGCGGGACGATTATTAAGGCGCGTTTACCAAAAGCGC
GTATTGGCGCCTTTTATAAGATAGAACCCAGTCAACGTTTAGCCGAGGTGATCGCTATTGATGAAGATGAGGTTTTCTTG
TTGCCTTTTGAGCATGTTTCCGGCATGTACTGTGGTCAGTGGTTAAGTTATCAGGGAGATGAGTTCAAGATTCGTGTTGG
AGACGCACTACTTGGGCGGCTTGTTGATGGCATAGGCAGACCAATGGAGAGTAATATTGCTGCCCCCTATCTTCCGTTTG
AACGCAGCTTGTATGCTGAACCACCAGATCCTTTATTAAGGCAGGTTATTGATCAGCCATTTATACTTGGCGTCAGGGCA
ATTGATGGGCTACTAACTTGCGGTATTGGGCAGCGTATCGGTATTTTTGCTGGTTCAGGCGTTGGTAAAAGTACGCTTTT
GGGGATGATTTGTAATGGCGCATCCGCAGATATTATTGTCCTTGCTCTTATCGGCGAACGTGGTCGCGAAGTAAATGAAT
TCCTCGCGCTCTTACCTCAATCCACGCTTTCTAAATGTGTACTGGTCGTCACAACGTCAGACCGCCCCGCGCTGGAAAGG
ATGAAAGCCGCATTTACGGCGACGACTATTGCAGAGTACTTCCGCGATCAAGGTAAAAATGTATTATTAATGATGGATTC
TGTAACTCGTTATGCCCGAGCCGCACGTGATGTTGGGCTTGCATCGGGAGAACCTGATGTAAGGGGGGGATTCCCTCCGA
GTGTTTTTTCTTCGTTACCCAAATTATTAGAGCGAGCGGGGCCTGCGCCAAAAGGTTCAATAACCGCGATTTATACGGTG
TTGTTAGAAAGCGATAATGTTAATGATCCTATTGGCGATGAAGTCCGCTCTATTCTTGATGGGCACATCGTACTTACCCG
AGAACTTGCAGAGGAAAACCATTTCCCTGCAATTGATATTGGTTTAAGTGCCAGTCGTGTTATGCATAACGTTGTTACAT
CGGAGCATTTGCGTGCGGCGGCAGAATGCAAAAAGCTTATTGCAACTTATAAAAATGTTGAGCTGCTTATTCGTATTGGT
GAGTACACGATGGGGCAAGATCCTGAAGCAGATAAGGCAATAAAAAATAGGAAATTAATTCAGAACTTTATACAACAAAG
CACCAAAGATATCAGTAGTTACGAAAAAACGATTGAAAGCCTATTCAAAGTGGTTGCCTGA

Upstream 100 bases:

>100_bases
TATGTGAAGAAAATGATTGAAGGTTCGTTCCCGTCAGTCCCCGTGCTCTCTTTTCAGGAGGTTGGGAATAATATCGAACT
TAAAGTATTAGGAACGGTAA

Downstream 100 bases:

>100_bases
GTATGTTGGACAGAATTTTATCTATTCGTAAAAGCAGAGCGAACCGATTGAGAGAATCAATGGCTAAGATTAACTCTCAG
ATAAAAGAGGTGGATGGTAA

Product: type III secretion apparatus H+-transporting two-sector ATPase

Products: NA

Alternate protein names: Yop proteins secretion ATPase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGTIIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFL
LPFEHVSGMYCGQWLSYQGDEFKIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALLPQSTLSKCVLVVTTSDRPALER
MKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAARDVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTV
LLESDNVNDPIGDEVRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKNVELLIRIG
EYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA

Sequences:

>Translated_446_residues
MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGTIIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFL
LPFEHVSGMYCGQWLSYQGDEFKIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALLPQSTLSKCVLVVTTSDRPALER
MKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAARDVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTV
LLESDNVNDPIGDEVRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKNVELLIRIG
EYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA
>Mature_446_residues
MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGTIIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFL
LPFEHVSGMYCGQWLSYQGDEFKIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALLPQSTLSKCVLVVTTSDRPALER
MKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAARDVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTV
LLESDNVNDPIGDEVRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKNVELLIRIG
EYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA

Specific function: Is a component of the Yop secretion machinery using ATP, and it is either the energizer of this machinery, or part of it [H]

COG id: COG1157

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway ATPase

Gene ontology:

Cell location: Cytoplasm. Note=But connected to the inner membrane possibly through LcrD (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI32189394, Length=367, Percent_Identity=31.6076294277929, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI19913424, Length=303, Percent_Identity=26.7326732673267, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI19913428, Length=375, Percent_Identity=26.4, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI50345984, Length=384, Percent_Identity=28.3854166666667, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI4757810, Length=384, Percent_Identity=28.3854166666667, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI19913426, Length=342, Percent_Identity=26.6081871345029, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788251, Length=430, Percent_Identity=40.4651162790698, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1790170, Length=407, Percent_Identity=27.7641277641278, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790172, Length=398, Percent_Identity=25.3768844221106, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790217, Length=238, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144756, Length=379, Percent_Identity=30.8707124010554, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17565854, Length=313, Percent_Identity=28.1150159744409, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71988063, Length=410, Percent_Identity=27.3170731707317, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71988080, Length=410, Percent_Identity=27.3170731707317, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17510931, Length=395, Percent_Identity=26.3291139240506, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17570191, Length=370, Percent_Identity=25.4054054054054, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988074, Length=312, Percent_Identity=29.4871794871795, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6322581, Length=418, Percent_Identity=27.7511961722488, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319370, Length=325, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319603, Length=397, Percent_Identity=26.9521410579345, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320016, Length=188, Percent_Identity=32.4468085106383, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24638766, Length=414, Percent_Identity=29.4685990338164, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28574560, Length=364, Percent_Identity=29.6703296703297, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20129479, Length=306, Percent_Identity=28.1045751633987, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24583988, Length=322, Percent_Identity=27.0186335403727, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583986, Length=322, Percent_Identity=27.0186335403727, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583984, Length=322, Percent_Identity=27.0186335403727, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583992, Length=336, Percent_Identity=26.1904761904762, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24658560, Length=418, Percent_Identity=26.3157894736842, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI281361666, Length=330, Percent_Identity=26.0606060606061, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24646341, Length=330, Percent_Identity=26.0606060606061, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17136796, Length=330, Percent_Identity=26.0606060606061, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR004100
- InterPro:   IPR005714
- InterPro:   IPR013380 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: =3.6.3.14 [H]

Molecular weight: Translated: 48817; Mature: 48817

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGTIIKARLPKARIGAFYK
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEEEEECCCHHHHCEEEE
IEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEFKIRVGDALLGRLVDGIGR
ECHHHHHHHHHEECCCCEEEEEHHHHCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCC
PMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRAIDGLLTCGIGQRIGIFAGSG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHCCCCCCEEEEECCC
VGKSTLLGMICNGASADIIVLALIGERGREVNEFLALLPQSTLSKCVLVVTTSDRPALER
CCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHCCEEEEEECCCCHHHHH
MKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAARDVGLASGEPDVRGGFPPSVFSSLP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KLLERAGPAPKGSITAIYTVLLESDNVNDPIGDEVRSILDGHIVLTRELAEENHFPAIDI
HHHHHCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHHHHCCCEEEEHHHHHCCCCCEEEE
GLSASRVMHNVVTSEHLRAAAECKKLIATYKNVELLIRIGEYTMGQDPEADKAIKNRKLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
QNFIQQSTKDISSYEKTIESLFKVVA
HHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGTIIKARLPKARIGAFYK
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCEEEEECCCHHHHCEEEE
IEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEFKIRVGDALLGRLVDGIGR
ECHHHHHHHHHEECCCCEEEEEHHHHCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCC
PMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRAIDGLLTCGIGQRIGIFAGSG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHCCCCCCEEEEECCC
VGKSTLLGMICNGASADIIVLALIGERGREVNEFLALLPQSTLSKCVLVVTTSDRPALER
CCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHCCEEEEEECCCCHHHHH
MKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAARDVGLASGEPDVRGGFPPSVFSSLP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KLLERAGPAPKGSITAIYTVLLESDNVNDPIGDEVRSILDGHIVLTRELAEENHFPAIDI
HHHHHCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHHHHCCCEEEEHHHHHCCCCCEEEE
GLSASRVMHNVVTSEHLRAAAECKKLIATYKNVELLIRIGEYTMGQDPEADKAIKNRKLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
QNFIQQSTKDISSYEKTIESLFKVVA
HHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8132449; 2160939 [H]