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The map label for this gene is amiB [H]

Identifier: 209396446

GI number: 209396446

Start: 5324990

End: 5326327

Strand: Direct

Name: amiB [H]

Synonym: ECH74115_5685

Alternate gene names: 209396446

Gene position: 5324990-5326327 (Clockwise)

Preceding gene: 209400173

Following gene: 209398397

Centisome position: 95.57

GC content: 55.01

Gene sequence:

>1338_bases
ATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGTTGCTGCTGCTGTGCGCGCAGGTGGGTGCCGCGACGCTCTCTGA
TATTCAGGTTTCTAACGGCAACCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATC
AAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGTAATAAT
CTGGTGAAGGCGATTCGCTCCGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAATGGTAA
AACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGCGCCGCCACCGCCTC
CTCCGCCGCCTGTGGTTGTGAAACGCGTTGAAACGCCTACGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCG
TTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAA
CAATGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGC
GGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTA
ACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGCGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTC
GATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGCG
AAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGCGGGGCGGGTGATGTGCTGGCGAACAGTCAG
TCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTCGGTCATTCCCAGCGGGTAGGGTATGATGTAGCGACCAG
TATGATCAGTCAGTTGCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGC
CGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTAC
CAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTATTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGG
TGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAA

Upstream 100 bases:

>100_bases
TTATCAGGCACAAGGCCGTGAGGCGCGCGTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCACGTTTAGCCGGTT
AACCTTTGAAAGGTGGTGGG

Downstream 100 bases:

>100_bases
GGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGC
GTCGGTAGTCAAAGAACTGG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNN
LVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVL
TRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQ
SDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDY
QQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN

Sequences:

>Translated_445_residues
MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNN
LVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVL
TRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQ
SDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDY
QQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN
>Mature_445_residues
MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNN
LVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVL
TRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQ
SDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDY
QQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=445, Percent_Identity=98.6516853932584, Blast_Score=898, Evalue=0.0,
Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=40.4444444444444, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788776, Length=244, Percent_Identity=38.1147540983607, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48030; Mature: 48030

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVA
CCCHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEE
LDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYT
EEECCCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCEE
VVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNPFKTESNRTTGVISSNTVTRP
EEEEEECCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
AARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVL
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEE
TRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLE
ECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHK
HHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNYFLAHP
CCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
MQSAPQGATAQTASTVTTPDRTLPN
CCCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVA
CCCHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEE
LDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYT
EEECCCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCEE
VVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNPFKTESNRTTGVISSNTVTRP
EEEEEECCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
AARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVL
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEE
TRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLE
ECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHK
HHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNYFLAHP
CCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
MQSAPQGATAQTASTVTTPDRTLPN
CCCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]