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The map label for this gene is treF [H]

Identifier: 209396219

GI number: 209396219

Start: 4514806

End: 4516455

Strand: Direct

Name: treF [H]

Synonym: ECH74115_4882

Alternate gene names: 209396219

Gene position: 4514806-4516455 (Clockwise)

Preceding gene: 209398218

Following gene: 209396325

Centisome position: 81.03

GC content: 54.06

Gene sequence:

>1650_bases
ATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAGGTCGATCTCTGCTATGAACTGGACCCGTA
TGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCCGCCTCTGATGCGCTGA
CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGAC
TGCGCACCCAAAATGGACCCGCTGGATATTTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA
GTTTGTTGAAAATCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGC
ATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAG
TCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACCATGCTGGGGCTGGCGGAAAG
TGGTCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCA
ACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGC
GGTGCGCGCCGCTATCTCGACCACCTGAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTGATCCCTAATCA
GGCCTATCGCCATGTTGTGCGGATGCCGGACGGATCGCTGCTCAACCGTTACTGGGACGATCGCGACACGCCGCGTGACG
AATCCTGGCTTGAGGACGTTGAAACCGCGAAACATTCTGGTCGCCCGCCCAACGAGGTGTACCGCGATTTACGCGCGGGG
GCGGCCTCCGGTTGGGATTACTCTTCCCGTTGGCTCCGTGATACTGGTCGTCTGGCGAGCATTCGTACCACCCAGTTCAT
CCCCATCGATCTGAATGCCTTCCTGTTTAAACTGGAGAGCGCCATCGCCAACATCTCGGCGCTGAAAGGTGAGAAAGAGA
CAGAAGCACTGTTCCGCCAGAAGGCCAGTGCCCGTCGCGATGCGGTAAACCGTTACCTCTGGGATGATGAAAACGGCATC
TACCGCGATTACGACTGGCGACGCGAACAACTGGCGCTGTTTTCCGCTGCCGCCATTGTGCCGCTCTATGTCGGTATGGC
GAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGT
ACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCAATTCAGGGATTTAAAATGTAT
GGCGATGACCTTCTGGGCGATGAAATCGCGCGCAGCTGGCTGAAGACGGTGAATCAGTTTTATCTGGAACAGCACAAACT
GATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCGGTGGCGAGTATCCGTTGCAGGATGGGTTTGGCT
GGACTAACGGTGTGGTACGCCGTTTAATTGGTTTGTACGGCGAACCATAA

Upstream 100 bases:

>100_bases
CGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCCGATAATTAGCGACGA
ATTTCGGAGGTTGGATCCTT

Downstream 100 bases:

>100_bases
TATTTTTACAGCCAGCCGCTAACTTCCTGCTGGCTGTAAAATTATCCTCTTCAGGAGGAGATATTTAACATCATTGCCGC
CTGGGTGCGATTTTTCACTT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Sequences:

>Translated_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP
>Mature_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=525, Percent_Identity=34.0952380952381, Blast_Score=254, Evalue=2e-67,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=100, Blast_Score=1131, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=51.129363449692, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.6888045540797, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25141398, Length=529, Percent_Identity=31.3799621928166, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17565078, Length=510, Percent_Identity=31.3725490196078, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI25148109, Length=546, Percent_Identity=30.03663003663, Blast_Score=231, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI71987755, Length=570, Percent_Identity=28.0701754385965, Blast_Score=193, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=473, Percent_Identity=27.6955602536998, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319473, Length=481, Percent_Identity=28.6902286902287, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24656680, Length=550, Percent_Identity=31.2727272727273, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI24656675, Length=550, Percent_Identity=31.2727272727273, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI24656661, Length=535, Percent_Identity=31.4018691588785, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17933716, Length=535, Percent_Identity=31.4018691588785, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656670, Length=535, Percent_Identity=31.4018691588785, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656685, Length=486, Percent_Identity=31.8930041152263, Blast_Score=219, Evalue=6e-57,
Organism=Drosophila melanogaster, GI22024178, Length=533, Percent_Identity=25.891181988743, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45551104, Length=376, Percent_Identity=26.063829787234, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28573474, Length=297, Percent_Identity=26.9360269360269, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63697; Mature: 63697

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC
>Mature Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA