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The map label for this gene is pepB

Identifier: 209396200

GI number: 209396200

Start: 3479847

End: 3481130

Strand: Reverse

Name: pepB

Synonym: ECH74115_3754

Alternate gene names: 209396200

Gene position: 3481130-3479847 (Counterclockwise)

Preceding gene: 209398449

Following gene: 209397515

Centisome position: 62.47

GC content: 56.07

Gene sequence:

>1284_bases
ATGACAGAAGCGATGAAGATTACCCTCTCTACCCAACCTGCCGACGCGCGCTGGGGAGAAAAAGCAACTTACAGCATTAA
TAATGACGGCATTACCCTGCATTTGAACGGGGCAGACGATCTGGGGCTGATCCAGCGTGCGGCGCGCAAGATTGACGGTC
TGGGCATCAAGCATGTTCAGTTAAGCGGTGAAGGCTGGGATGCGGATCGATGCTGGGCATTCTGGCAAGGTTACAAAGCC
CCGAAAGGCACGCGTAAAGTGGAGTGGCCGGATCTGGACGATGCCCAGCGCCAGGAACTGGATAACCGCCTGATGATCAT
CGACTGGGTGCGTGACACCATCAACGCACCGGCAGAAGAATTGGGACCATCGCAACTGGCACAGCGTGCTGTTGATCTGA
TCAGCAACGTCGCGAGCGATCGTGTGACTTATCGGATCACCAAAGGCGAAGATCTGCGTGAGCAAGGTTATATGGGGCTG
CACACCGTCGGACGCGGTTCAGAACGTTCTCCGGTATTGCTGGCGCTGGATTACAACCCAACTGGCGATAAAGAAGCGCC
AGTGTACGCGTGCCTGGTAGGTAAAGGTATCACTTTTGACTCCGGCGGCTACAGCATCAAACAGACTGCGTTTATGGACT
CGATGAAGTCGGACATGGGCGGCGCGGCAACGGTTACCGGGGCGCTGGCATTTGCCATTACGCGCGGACTGAACAAGCGC
GTGAAGCTGTTCCTCTGCTGTGCGGATAACCTGATTAGCGGCAATGCGTTCAAGCTGGGCGATATCATCACTTATCGCAA
CGGTAAAAAAGTTGAAGTGATGAACACTGATGCCGAAGGGCGCCTGGTGCTTGCCGATGGTCTGATTGATGCCAGTGCGC
AGAAACCGGAAATGATCATTGATGCGGCGACCCTCACCGGGGCGGCGAAAACTGCGCTGGGTAATGATTATCACGCGCTG
TTCAGTTTTGACGATGCGCTTGCCGGTCGTTTGCTGGCGAGTGCCTCACAAGAGAACGAACCATTCTGGCGTCTGCCGCT
GGCGGAATTCCACCGCAGCCAGCTGCCGTCTAACTTTGCCGAACTGAACAATACCGGAAGCGCGGCGTATCCGGCAGGCG
CGAGCACGGCAGCGGGCTTCCTGTCGCACTTTGTTGAGAACTATCAGCAAGGCTGGCTGCATATCGACTGCTCGGCGACT
TACCGTAAAGCGCCGGTTGAACAGTGGTCTGCGGGTGCTACGGGACTTGGTGTGCGCACGATTGCTAATCTGTTAACGGC
GTAA

Upstream 100 bases:

>100_bases
GGGCTTCACTCACTTGCCGCCTTCCTGCAACGCGAATCATTTAGCGGAAAAATCCTGGGGCTGCCAACTGGCGGCCCTTT
TACAAAGAAGGATAACTAAA

Downstream 100 bases:

>100_bases
ACTAATATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAGTATTGCAAATTCAACAAATTGTGAATCCCTTGTA
GGCCTGATAAGTATGGCGCA

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA

Sequences:

>Translated_427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA
>Mature_426_residues
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAP
KGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGLH
TVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKRV
KLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALF
SFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATY
RKAPVEQWSAGATGLGVRTIANLLTA

Specific function: Probably plays an important role in intracellular peptide degradation

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family

Homologues:

Organism=Homo sapiens, GI41393561, Length=317, Percent_Identity=36.9085173501577, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI47155554, Length=286, Percent_Identity=35.6643356643357, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=99.2974238875878, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1790710, Length=315, Percent_Identity=38.4126984126984, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17556903, Length=325, Percent_Identity=32.6153846153846, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17565172, Length=207, Percent_Identity=28.5024154589372, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221379063, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221379062, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357381, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24662227, Length=322, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21355725, Length=289, Percent_Identity=29.4117647058824, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24661038, Length=320, Percent_Identity=28.125, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161077148, Length=344, Percent_Identity=28.4883720930233, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20130057, Length=344, Percent_Identity=28.4883720930233, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129969, Length=294, Percent_Identity=29.2517006802721, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21355645, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24662223, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129963, Length=316, Percent_Identity=27.5316455696203, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922386, Length=311, Percent_Identity=27.0096463022508, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24646701, Length=228, Percent_Identity=29.3859649122807, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646703, Length=228, Percent_Identity=29.3859649122807, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21358201, Length=228, Percent_Identity=29.3859649122807, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPB_ECO57 (P58473)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85897
- PIR:   E91052
- RefSeq:   NP_289080.2
- RefSeq:   NP_311416.2
- ProteinModelPortal:   P58473
- SMR:   P58473
- EnsemblBacteria:   EBESCT00000024201
- EnsemblBacteria:   EBESCT00000058532
- GeneID:   912754
- GeneID:   959184
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3790
- KEGG:   ecs:ECs3389
- GeneTree:   EBGT00050000011358
- HOGENOM:   HBG742580
- OMA:   ENEAFWR
- ProtClustDB:   PRK05015
- BioCyc:   ECOL83334:ECS3389-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00504
- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819
- PANTHER:   PTHR11963:SF3
- PIRSF:   PIRSF036388
- PRINTS:   PR00481

Pfam domain/function: PF00883 Peptidase_M17

EC number: =3.4.11.23

Molecular weight: Translated: 46257; Mature: 46125

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00631 CYTOSOL_AP

Important sites: ACT_SITE 207-207 ACT_SITE 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC
>Mature Secondary Structure 
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796