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The map label for this gene is spaL [H]

Identifier: 209396176

GI number: 209396176

Start: 3832304

End: 3833596

Strand: Reverse

Name: spaL [H]

Synonym: ECH74115_4146

Alternate gene names: 209396176

Gene position: 3833596-3832304 (Counterclockwise)

Preceding gene: 209398095

Following gene: 209396721

Centisome position: 68.8

GC content: 44.55

Gene sequence:

>1293_bases
ATGAAAAAATTAAAATTATTAAATAAATACAGCTACTTACATAGCATAAACGGCTCTCTGATTGAAGCAGAACTTGACGA
CGTTTCTGTGGGTGAAGTATGCGAAATTTATGCGAGCAGGCAAGCAAATGAACGTATAGCCAGGGCTCAGGTCGTTGGCT
TTCGCAATGGGAAGACATTATTAAACCTTATAGGCAGCTCTGTGGGGCTTACACGTACTGCAGTTTTGAAACCAACGGGA
GAGCAGTTAACCATACAGATCAGTGATGCCTTTCTTGGTTCGGTTTTGAATGCCTCTGGTCAAATTATGGAAAGGTTTGT
CCCGGACACTCCAGGCGATAGGGGAAATTTACGCCTGATTGATGAACTGCCACCGTCGTATCAGGAACGTCGAGTTATTA
ATACACCACTTGAGACACGGATAAGAGTCATTGATGGCGTACTGACATGTGGCATTGGTCAACGTGTTGGGATTTTCGCC
TCGGCTGGCTGTGGCAAAACGGTTTTAATGCATATGCTCGTAAACAATACTGAAGCTGATGTGTTTGTTATTGGCTTGAT
TGGCGAACGTGGTAGGGAAGTTACGGAATGCGCGGAATCGCTCAAAAAATCAGTAAATGCAGCGAAATGCGTATTGGTCT
ATGCCACCTCTGATTTTTCGTCAGTAGACCGGTGTAATGCCGCCCTGATGGCGACAACCGTGGCAGAGTATTTTCGAGAT
CGAGGAAAGAGAGTTGTACTTTTTATCGACTCAATGACGCGGTATGCACGAGCACTACGTGATATGAAACTGGCAGCGGG
TGAACCACCTGCCAGACGTGGTTATCCTGCTTCTGTTTTTGACAGTTTGCCTCGCTTACTGGAGAGGCCGGGACCAACAC
TAAAAGGAAGTATTACCGAGTTTTATACCGTGTTACTGGAAGGGGAAGATGAATCAGACCCACTGGGTGACGAAATTCGC
TCAATCCTTGATGGCCATATCTATCTAAGTCGCAAACTCGCGGGTCAAGGTCACTATCCGGCAATAGATGTTCTAAAAAG
TGTGAGTCGAGTATTTGGTCAGGTCACTGATGAAAAACACAGAGATAATGCTGCACGGGTAAGGAAGAACCTCACAACTT
TGGAAGATCTACAGGTGTTTATTGACTTAGGGGAATATCGCGCAGGGCAAAATGCTGAAAATGATTTTGCTATGAATGCC
CGGCCTAAACTCACAAATTGGTTGAAACAGTCAGTTAATGAAAAAATGCCAATGAGTGAAACGCTAAAAGAGCTTGAACG
AATTGTTAAGTAA

Upstream 100 bases:

>100_bases
GACCTCGAAGTACTTTCGTACGGGGAACTAACGGAAAATGTTCCCGTTAATATTCTGAAAACTATTTAGGATAATGTCTT
GCGATATGGAGCATGCTGGT

Downstream 100 bases:

>100_bases
AGTTAATCGACTTATCAGAAGAACAGCGCAGAGCCTGGCTGCATGTGAAGCATCATTACAAAAGTTAAATGCAGAAAAAG
AAAAGTTAGCCGAAAAGGAA

Product: ATP synthase SpaL

Products: NA

Alternate protein names: Invasion protein InvC [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTG
EQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAEYFRD
RGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIR
SILDGHIYLSRKLAGQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNA
RPKLTNWLKQSVNEKMPMSETLKELERIVK

Sequences:

>Translated_430_residues
MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTG
EQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAEYFRD
RGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIR
SILDGHIYLSRKLAGQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNA
RPKLTNWLKQSVNEKMPMSETLKELERIVK
>Mature_430_residues
MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTG
EQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAEYFRD
RGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIR
SILDGHIYLSRKLAGQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNA
RPKLTNWLKQSVNEKMPMSETLKELERIVK

Specific function: Necessary for efficient entry of S.typhimurium into cultured epithelial cells. Probable catalytic subunit of a protein translocase. May energize the protein export apparatus encoded in the inv locus which is required for the surface presentation of determ

COG id: COG1157

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway ATPase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI32189394, Length=390, Percent_Identity=28.7179487179487, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI19913424, Length=238, Percent_Identity=32.3529411764706, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI50345984, Length=362, Percent_Identity=25.9668508287293, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4757810, Length=362, Percent_Identity=25.9668508287293, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI19913428, Length=406, Percent_Identity=25.8620689655172, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI19913426, Length=376, Percent_Identity=26.3297872340426, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788251, Length=442, Percent_Identity=38.4615384615385, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1790170, Length=370, Percent_Identity=31.0810810810811, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790172, Length=382, Percent_Identity=25.6544502617801, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144756, Length=325, Percent_Identity=32.6153846153846, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565854, Length=243, Percent_Identity=32.9218106995885, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71988080, Length=360, Percent_Identity=26.9444444444444, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988063, Length=360, Percent_Identity=26.9444444444444, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17510931, Length=395, Percent_Identity=25.8227848101266, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17570191, Length=393, Percent_Identity=25.1908396946565, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988074, Length=308, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322581, Length=325, Percent_Identity=31.6923076923077, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319370, Length=381, Percent_Identity=27.2965879265092, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319603, Length=371, Percent_Identity=24.5283018867925, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320016, Length=239, Percent_Identity=30.1255230125523, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574560, Length=402, Percent_Identity=30.5970149253731, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24638766, Length=386, Percent_Identity=30.5699481865285, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129479, Length=272, Percent_Identity=31.6176470588235, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583992, Length=288, Percent_Identity=31.25, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24658560, Length=359, Percent_Identity=28.133704735376, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583988, Length=288, Percent_Identity=30.9027777777778, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583986, Length=288, Percent_Identity=30.9027777777778, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583984, Length=288, Percent_Identity=30.9027777777778, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281361666, Length=393, Percent_Identity=24.4274809160305, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646341, Length=393, Percent_Identity=24.4274809160305, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136796, Length=393, Percent_Identity=24.4274809160305, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR004100
- InterPro:   IPR005714 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: =3.6.3.14 [H]

Molecular weight: Translated: 47600; Mature: 47600

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKTL
CCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHH
LNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLI
HHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEE
DELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGKTVLMHMLVNNTEAD
CCCCCCHHHCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
VFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAEYFRD
EEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
RGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTLKGSITE
CCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
FYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYPAIDVLKSVSRVFGQVTDEKH
HEEEEECCCCCCCCCHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHH
RDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNARPKLTNWLKQSVNEKMPMSE
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
TLKELERIVK
HHHHHHHHHC
>Mature Secondary Structure
MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKTL
CCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHH
LNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLI
HHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEE
DELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGKTVLMHMLVNNTEAD
CCCCCCHHHCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
VFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAEYFRD
EEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
RGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTLKGSITE
CCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
FYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYPAIDVLKSVSRVFGQVTDEKH
HEEEEECCCCCCCCCHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHH
RDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNARPKLTNWLKQSVNEKMPMSE
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
TLKELERIVK
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]