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The map label for this gene is gcvT [H]

Identifier: 209395879

GI number: 209395879

Start: 1299777

End: 1300937

Strand: Direct

Name: gcvT [H]

Synonym: ECH74115_1287

Alternate gene names: 209395879

Gene position: 1299777-1300937 (Clockwise)

Preceding gene: 209400702

Following gene: 209398258

Centisome position: 23.33

GC content: 49.27

Gene sequence:

>1161_bases
ATGAAATCTCTTGCTGAATTTCACTTGAAAAATAATGCGGTAATGGGGGTATATAACAACCGTACCCTACCTTCTTCTTA
TCACGATGCTATGACCGAATATAAAGCCGTTCGTGAAAACGCGCTGCTGGTGGACTATTCTCACCTCTCTATTGTTAGCG
TCATGGGCGATGATGCCTGGGCGCTGATCAACCAGCTGGTTTCCGCAGATGTCTCCATCATTCGTGATGAGCAGGCAATC
TACTCACTGGTGTTGAATGAAGAGGGCACCATTCGCGGAGACGTCTATGTGCTGTGCAGCATTGACGGTTATTACCTGCT
TTCAGAAGATATTTCGGCTGCCGAATTGATCGCCAGCATGAATACCATTCTCGAAAAAGCGGAAGAACTGGATATTCAAT
CGATGCCTGAAATTCAGGATATGCGGGAAAACAACTGGGGGGCAATCCTGCTCGAAGGCCCATACTCCTGGGAGATCATG
TCTGAAATTCATGGCTTTGACGTGATTGGCCTGCCTTACTACGAATACATGAATACCGAGGAGGATCTGCTGCTCTTCCG
CTGTGGTAAACACGGTGAGTATGCGTACATGACCATCGGTGAGCAGGCGAAACTGGCGGAGCAGTGGGAAAAACTGTTAA
CCGTTGGCGAGAAATACCTGATGCAGACCGGCGGCCTGGATTATCAAAAAATCGTACGCCTCGAAAATCCGTGCTGGGAT
GCCAGCCTCTGGGAAGGTCAGGCGGTGAATCCGGTTCAGTTGCAGATGCAGTGGGCGGTGCAGTACGACAAAGATGATTT
TATTGGCAAGGACGCGGTGACAGAGCTATCCCAGGAGTATACCGGTAATAAACTCATCGGCATGATCGCACAGGAAGAAT
GCGAAGGCATTGAGGCTGGCGATCGCGTGCTGGTGGAAGGTCAAGACGTGGGCTATGTCGTGAAGGCACTCTTTTCCCCA
GCGTTGCAGCGTTTCATTGCCCTGACCCTGCTGGAAAAGGATTACGCCTGGTCTGACATCAGCGGCTACGAAATTCAAAC
TGCTCACGGAATTATTCCGGCGCAATCAAAATGTATGCCGTTTATTTATAACCTGAGCATGTTGGTTAGCCCAACTGAGC
ACAGTTATATCGACGCGTCAAAAAATAAAAGCGCAGCGTAA

Upstream 100 bases:

>100_bases
ACCCGTCCTATATGCGCAGTATCAATAGTCTGGCCACCTTCGTTATTCAAAAGAAAAACGATTAACTACTTATTAAAGAA
TTAATTATCAGGACACGTTT

Downstream 100 bases:

>100_bases
TGAATATCGCCGCGTGACTATAGCTTACGCGGCTTAAATTAACCCCAATTGGCGGGATTGCCAGGTATTATTCATGAAAA
CGAACAGGAAAAGAGTTGTT

Product: putative aminomethyltransferase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLVDYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAI
YSLVLNEEGTIRGDVYVLCSIDGYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEKYLMQTGGLDYQKIVRLENPCWD
ASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTELSQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSP
ALQRFIALTLLEKDYAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA

Sequences:

>Translated_386_residues
MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLVDYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAI
YSLVLNEEGTIRGDVYVLCSIDGYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEKYLMQTGGLDYQKIVRLENPCWD
ASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTELSQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSP
ALQRFIALTLLEKDYAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA
>Mature_386_residues
MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLVDYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAI
YSLVLNEEGTIRGDVYVLCSIDGYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEKYLMQTGGLDYQKIVRLENPCWD
ASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTELSQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSP
ALQRFIALTLLEKDYAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI257796258, Length=346, Percent_Identity=23.9884393063584, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI44662838, Length=346, Percent_Identity=23.9884393063584, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789272, Length=374, Percent_Identity=23.7967914438503, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 43515; Mature: 43515

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLVDYSHLSIVSVMGDDAW
CCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCHH
ALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSIDGYYLLSEDISAAELIASM
HHHHHHHHCCHHHEEHHHHHHHEEECCCCCEECCEEEEEECCCEEEECCCCHHHHHHHHH
NTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIMSEIHGFDVIGLPYYEYMNTE
HHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCHHHHCCCC
EDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEKYLMQTGGLDYQKIVRLENPCWD
CCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
ASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTELSQEYTGNKLIGMIAQEECEGIEAG
CCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCC
DRVLVEGQDVGYVVKALFSPALQRFIALTLLEKDYAWSDISGYEIQTAHGIIPAQSKCMP
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
FIYNLSMLVSPTEHSYIDASKNKSAA
EEEEEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLVDYSHLSIVSVMGDDAW
CCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCHH
ALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSIDGYYLLSEDISAAELIASM
HHHHHHHHCCHHHEEHHHHHHHEEECCCCCEECCEEEEEECCCEEEECCCCHHHHHHHHH
NTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIMSEIHGFDVIGLPYYEYMNTE
HHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCHHHHCCCC
EDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEKYLMQTGGLDYQKIVRLENPCWD
CCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
ASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTELSQEYTGNKLIGMIAQEECEGIEAG
CCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCC
DRVLVEGQDVGYVVKALFSPALQRFIALTLLEKDYAWSDISGYEIQTAHGIIPAQSKCMP
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
FIYNLSMLVSPTEHSYIDASKNKSAA
EEEEEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]