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The map label for this gene is CbiB [H]

Identifier: 20806889

GI number: 20806889

Start: 402033

End: 402983

Strand: Direct

Name: CbiB [H]

Synonym: TTE0377

Alternate gene names: 20806889

Gene position: 402033-402983 (Clockwise)

Preceding gene: 20806888

Following gene: 20806890

Centisome position: 14.95

GC content: 35.54

Gene sequence:

>951_bases
ATGAAAGTTTTTCTGGCATATATTCTCGATTTGATTTTAGGAGACCCTGAGGGTTATCCTCATCCTGTAAGGCTGATAGG
ATCTCTTATATCAAAAGAAGAAAGAATAATAAGAAAATACGCAAAAGGTGAAAGAGCTTTAAAAATTGCAGGATTTGTCA
TGTGTTTTTTGACAATTGCTACAGTATATTTTACCACTTACTTTGCCCTTTATTTAGCGGGAATTATAAATCGTTACTTC
CAATACTTGCTCGAAGTAGTATTTATCTATACGACTCTTGCGACAAAAGACCTGGCAAAAGCGGCTTACAGAGTTTTTAA
ACCTTTAGAAGAGAAAGATTTAGAAACTGCTCGGAAAAAACTTTCTCTCATAGTGAGCAGGGATACTGAAAAATTAGAGG
AAAAAGACATAATAAGAGGAGTTTTGGAGACAGTTTCAGAAAATATCTCAGACGGAATAATAGCTCCGATGTTTTACGCA
TTTTTAGGAGGAGCCCCTCTTGCAATGCTTTATAAAGCTGCAAGTACCCTGGATTCTATGGTGGGATATAAAAATGAAAA
GTACAAAGACCTAGGCTTTGCTTCTGCTAAATTAGATGATATTTTGAACTTCATACCTGCGAGAATAACAGGGGTTTTAA
TAGTAGTCTCTTCCTTTTTACTGGGTTATGACTACAAAAACAGTTTCAGGATTTTTATGAGGGATAGGTTAAAGCACGAA
AGTCCCAATAGCGCCCATGGGGAAGCTGCTTTGGCTGGAGCGCTGGGTGTAGAGCTGGGAGGGATAAATTACTATTTTGG
AAAGCCTGTTATAAAGCCCAGGTTAGGAGACAAAAAAGTTGATTTATCTTTAAAACACATAAAAGACAGCGTAAAAATAA
TGTATATGACCTCTTTTATAGGGTTAATCGTATTTTATCTACTCGAAAATTTCTTTAAAAAATTAATTTGA

Upstream 100 bases:

>100_bases
GAGAATTTAGAGAAAAAGAATATGACAGGCTTGCAAATATTGTGAGAAACTCCCTTGACATGGAAAGGATATACCAGATA
ATGGAGAGGTATAGAGACAA

Downstream 100 bases:

>100_bases
GAGGGGAGATTTGAATGAAAGAAAAAGAGGTATCTTTAGGGAATGTGAAGACTCTCACTTTAGTTGCCATGTTAATAGCT
CTGTCAGCTGTAGGGGCTTT

Product: cobalamin biosynthesis protein CobD/CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKVFLAYILDLILGDPEGYPHPVRLIGSLISKEERIIRKYAKGERALKIAGFVMCFLTIATVYFTTYFALYLAGIINRYF
QYLLEVVFIYTTLATKDLAKAAYRVFKPLEEKDLETARKKLSLIVSRDTEKLEEKDIIRGVLETVSENISDGIIAPMFYA
FLGGAPLAMLYKAASTLDSMVGYKNEKYKDLGFASAKLDDILNFIPARITGVLIVVSSFLLGYDYKNSFRIFMRDRLKHE
SPNSAHGEAALAGALGVELGGINYYFGKPVIKPRLGDKKVDLSLKHIKDSVKIMYMTSFIGLIVFYLLENFFKKLI

Sequences:

>Translated_316_residues
MKVFLAYILDLILGDPEGYPHPVRLIGSLISKEERIIRKYAKGERALKIAGFVMCFLTIATVYFTTYFALYLAGIINRYF
QYLLEVVFIYTTLATKDLAKAAYRVFKPLEEKDLETARKKLSLIVSRDTEKLEEKDIIRGVLETVSENISDGIIAPMFYA
FLGGAPLAMLYKAASTLDSMVGYKNEKYKDLGFASAKLDDILNFIPARITGVLIVVSSFLLGYDYKNSFRIFMRDRLKHE
SPNSAHGEAALAGALGVELGGINYYFGKPVIKPRLGDKKVDLSLKHIKDSVKIMYMTSFIGLIVFYLLENFFKKLI
>Mature_316_residues
MKVFLAYILDLILGDPEGYPHPVRLIGSLISKEERIIRKYAKGERALKIAGFVMCFLTIATVYFTTYFALYLAGIINRYF
QYLLEVVFIYTTLATKDLAKAAYRVFKPLEEKDLETARKKLSLIVSRDTEKLEEKDIIRGVLETVSENISDGIIAPMFYA
FLGGAPLAMLYKAASTLDSMVGYKNEKYKDLGFASAKLDDILNFIPARITGVLIVVSSFLLGYDYKNSFRIFMRDRLKHE
SPNSAHGEAALAGALGVELGGINYYFGKPVIKPRLGDKKVDLSLKHIKDSVKIMYMTSFIGLIVFYLLENFFKKLI

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35711; Mature: 35711

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFLAYILDLILGDPEGYPHPVRLIGSLISKEERIIRKYAKGERALKIAGFVMCFLTIA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVYFTTYFALYLAGIINRYFQYLLEVVFIYTTLATKDLAKAAYRVFKPLEEKDLETARKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSLIVSRDTEKLEEKDIIRGVLETVSENISDGIIAPMFYAFLGGAPLAMLYKAASTLDSM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VGYKNEKYKDLGFASAKLDDILNFIPARITGVLIVVSSFLLGYDYKNSFRIFMRDRLKHE
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
SPNSAHGEAALAGALGVELGGINYYFGKPVIKPRLGDKKVDLSLKHIKDSVKIMYMTSFI
CCCCCCCHHHHHHHHHHEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLIVFYLLENFFKKLI
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVFLAYILDLILGDPEGYPHPVRLIGSLISKEERIIRKYAKGERALKIAGFVMCFLTIA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVYFTTYFALYLAGIINRYFQYLLEVVFIYTTLATKDLAKAAYRVFKPLEEKDLETARKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSLIVSRDTEKLEEKDIIRGVLETVSENISDGIIAPMFYAFLGGAPLAMLYKAASTLDSM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VGYKNEKYKDLGFASAKLDDILNFIPARITGVLIVVSSFLLGYDYKNSFRIFMRDRLKHE
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
SPNSAHGEAALAGALGVELGGINYYFGKPVIKPRLGDKKVDLSLKHIKDSVKIMYMTSFI
CCCCCCCHHHHHHHHHHEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLIVFYLLENFFKKLI
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA