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The map label for this gene is CobB

Identifier: 20806887

GI number: 20806887

Start: 399150

End: 400508

Strand: Direct

Name: CobB

Synonym: TTE0375

Alternate gene names: 20806887

Gene position: 399150-400508 (Clockwise)

Preceding gene: 20806886

Following gene: 20806888

Centisome position: 14.84

GC content: 38.48

Gene sequence:

>1359_bases
TTGTGTAGAGTTTTCATGATAGCTGGAACCCATTCGGGGGCAGGCAAGACTACTTTAAGTCTTGGGATCATGGGCGTTCT
TTCAAAGAAGTATAATGTTAGGCCTTTCAAAGTTGGACCTGACTATATAGATACTGCCTACCACAGGTATGTGACAAGGA
ATTTTTCAGTGAATCTCGACCTTTTTATGCTGGGAGAGGAGAATCTCAAAAATTTGTTTTATAAAAATGCATCACGTGCG
GATGTTTCAATAATAGAAGGGGTCATGGGGCTTTACGACGGGATAGATACGACATCAAGAGGAAGCAGTGCCCACATAGC
AAAGCTTGTAAATGTGCCTGTTGTGCTCGTGGTGGATGCTTCTTCTATGGCAGCTAGTGTCTCGGCTTTAATAATGGGAT
ATATCTATTACGACAAAGATGTAGACATAAGAGGAGTTATTCTGAATAAAGTTGGAAGTGAAAGGCACTACACCCTCTTA
AAAGAGGTTATAGAGAGGGATTTGAATATTGAAGTTTTTGGTTATCTTCCTAAAGATGTGGAACTTGAGCTTCCTGAAAG
GCATTTAGGACTTTTACCTGTATATGAGACAGAAAATTTAGATAAAAAATTGGAAAAATTATATGGCTATATTGAAAATT
GCATTGATGTGGAAAAATTGATGAATTTGACAGTTAAGCCGCCTGATTTTATTGAAAAGGAAGATAGAGTCCTGAAAGAG
AAAAAGGTAAAAATAGCTTATGCTTATGACGAGGCTTTTAACTTTTACTACAAAGAAAGTCTTGAAACCTTAGAGGAAAT
GGGGGCACATCTTATTCCTTTCAGCCCTCTTGATGATGAAAGACTTCCAGAAGGAACTGAGGGACTTTACATTGGGGGTG
GGTTTCCAGAGGTTTTTGCGAAAAGGCTCAGTGAAAATGAGAGTATGTTGAGAGAAATAAAAGAAGCTGTTGAAAAAGGC
ATGCCTGTTTACGCTGAATGCGGGGGACTTATGTATCTTTCAAAAGGCATAAGGGATTTAGAGGGGAATAGATACAGCAT
GGTGGGAGTTTACGACTTTGAAGTAGTGATGACAAAAAAATTGCAGAGGTTTGGATATGTGGAAGCTGAAATTACCATGG
ATAATGTGCTCTTCAAAAAAGGTGAAAGGATAAAGGGACATGAGTTTCACTATTCTCGCATAGAAGGTTTTTCACAAAAT
GCCTCTTATATTGTGAATAGACCGGGCAAGGAAGAGAAGTGGGAATGTGGATTTGTGCATAAAAACTGTCTCGCAAGTTT
TGTTCACATAAACTTATACACATACAAAGAAGGAGTGAAGAGATTTTTAGACAGGTGTTCATCTTTTGGGAGGAAATAG

Upstream 100 bases:

>100_bases
ATAAAACTGGCATTTTCAATAGATGTTCAGATATTTGAAGACCCGATTACCAGAACCCCTTACATCATACCTTCTTTAAA
TGGAAAGGAGAAGCTGAAAG

Downstream 100 bases:

>100_bases
TATGGCGTTGAAACTTATGATACAGGGTACGGCCTCTTCAGTGGGTAAAAGCCTTATTGTCACAGCTTTGTGCAGGATAT
TCAAACAGGATGGACTTAAA

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MCRVFMIAGTHSGAGKTTLSLGIMGVLSKKYNVRPFKVGPDYIDTAYHRYVTRNFSVNLDLFMLGEENLKNLFYKNASRA
DVSIIEGVMGLYDGIDTTSRGSSAHIAKLVNVPVVLVVDASSMAASVSALIMGYIYYDKDVDIRGVILNKVGSERHYTLL
KEVIERDLNIEVFGYLPKDVELELPERHLGLLPVYETENLDKKLEKLYGYIENCIDVEKLMNLTVKPPDFIEKEDRVLKE
KKVKIAYAYDEAFNFYYKESLETLEEMGAHLIPFSPLDDERLPEGTEGLYIGGGFPEVFAKRLSENESMLREIKEAVEKG
MPVYAECGGLMYLSKGIRDLEGNRYSMVGVYDFEVVMTKKLQRFGYVEAEITMDNVLFKKGERIKGHEFHYSRIEGFSQN
ASYIVNRPGKEEKWECGFVHKNCLASFVHINLYTYKEGVKRFLDRCSSFGRK

Sequences:

>Translated_452_residues
MCRVFMIAGTHSGAGKTTLSLGIMGVLSKKYNVRPFKVGPDYIDTAYHRYVTRNFSVNLDLFMLGEENLKNLFYKNASRA
DVSIIEGVMGLYDGIDTTSRGSSAHIAKLVNVPVVLVVDASSMAASVSALIMGYIYYDKDVDIRGVILNKVGSERHYTLL
KEVIERDLNIEVFGYLPKDVELELPERHLGLLPVYETENLDKKLEKLYGYIENCIDVEKLMNLTVKPPDFIEKEDRVLKE
KKVKIAYAYDEAFNFYYKESLETLEEMGAHLIPFSPLDDERLPEGTEGLYIGGGFPEVFAKRLSENESMLREIKEAVEKG
MPVYAECGGLMYLSKGIRDLEGNRYSMVGVYDFEVVMTKKLQRFGYVEAEITMDNVLFKKGERIKGHEFHYSRIEGFSQN
ASYIVNRPGKEEKWECGFVHKNCLASFVHINLYTYKEGVKRFLDRCSSFGRK
>Mature_452_residues
MCRVFMIAGTHSGAGKTTLSLGIMGVLSKKYNVRPFKVGPDYIDTAYHRYVTRNFSVNLDLFMLGEENLKNLFYKNASRA
DVSIIEGVMGLYDGIDTTSRGSSAHIAKLVNVPVVLVVDASSMAASVSALIMGYIYYDKDVDIRGVILNKVGSERHYTLL
KEVIERDLNIEVFGYLPKDVELELPERHLGLLPVYETENLDKKLEKLYGYIENCIDVEKLMNLTVKPPDFIEKEDRVLKE
KKVKIAYAYDEAFNFYYKESLETLEEMGAHLIPFSPLDDERLPEGTEGLYIGGGFPEVFAKRLSENESMLREIKEAVEKG
MPVYAECGGLMYLSKGIRDLEGNRYSMVGVYDFEVVMTKKLQRFGYVEAEITMDNVLFKKGERIKGHEFHYSRIEGFSQN
ASYIVNRPGKEEKWECGFVHKNCLASFVHINLYTYKEGVKRFLDRCSSFGRK

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_THETN (Q8RCP4)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622058.1
- ProteinModelPortal:   Q8RCP4
- GeneID:   997281
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0375
- NMPDR:   fig|273068.3.peg.304
- HOGENOM:   HBG634910
- OMA:   HHTAICG
- ProtClustDB:   PRK01077
- BioCyc:   TTEN273068:TTE0375-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: NA

Molecular weight: Translated: 51555; Mature: 51555

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 327-327 ACT_SITE 429-429

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRVFMIAGTHSGAGKTTLSLGIMGVLSKKYNVRPFKVGPDYIDTAYHRYVTRNFSVNLD
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCEEEEEE
LFMLGEENLKNLFYKNASRADVSIIEGVMGLYDGIDTTSRGSSAHIAKLVNVPVVLVVDA
EEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECC
SSMAASVSALIMGYIYYDKDVDIRGVILNKVGSERHYTLLKEVIERDLNIEVFGYLPKDV
HHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
ELELPERHLGLLPVYETENLDKKLEKLYGYIENCIDVEKLMNLTVKPPDFIEKEDRVLKE
EEECCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHH
KKVKIAYAYDEAFNFYYKESLETLEEMGAHLIPFSPLDDERLPEGTEGLYIGGGFPEVFA
HCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KRLSENESMLREIKEAVEKGMPVYAECGGLMYLSKGIRDLEGNRYSMVGVYDFEVVMTKK
HHHCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCHHHCCCCEEEEEEEEEHHHHHHHH
LQRFGYVEAEITMDNVLFKKGERIKGHEFHYSRIEGFSQNASYIVNRPGKEEKWECGFVH
HHHCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCHH
KNCLASFVHINLYTYKEGVKRFLDRCSSFGRK
HHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCRVFMIAGTHSGAGKTTLSLGIMGVLSKKYNVRPFKVGPDYIDTAYHRYVTRNFSVNLD
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCEEEEEE
LFMLGEENLKNLFYKNASRADVSIIEGVMGLYDGIDTTSRGSSAHIAKLVNVPVVLVVDA
EEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECC
SSMAASVSALIMGYIYYDKDVDIRGVILNKVGSERHYTLLKEVIERDLNIEVFGYLPKDV
HHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
ELELPERHLGLLPVYETENLDKKLEKLYGYIENCIDVEKLMNLTVKPPDFIEKEDRVLKE
EEECCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHH
KKVKIAYAYDEAFNFYYKESLETLEEMGAHLIPFSPLDDERLPEGTEGLYIGGGFPEVFA
HCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KRLSENESMLREIKEAVEKGMPVYAECGGLMYLSKGIRDLEGNRYSMVGVYDFEVVMTKK
HHHCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCHHHCCCCEEEEEEEEEHHHHHHHH
LQRFGYVEAEITMDNVLFKKGERIKGHEFHYSRIEGFSQNASYIVNRPGKEEKWECGFVH
HHHCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCHH
KNCLASFVHINLYTYKEGVKRFLDRCSSFGRK
HHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336