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The map label for this gene is dus1 [H]

Identifier: 20806861

GI number: 20806861

Start: 368367

End: 369335

Strand: Direct

Name: dus1 [H]

Synonym: TTE0344

Alternate gene names: 20806861

Gene position: 368367-369335 (Clockwise)

Preceding gene: 20806860

Following gene: 20806864

Centisome position: 13.7

GC content: 37.87

Gene sequence:

>969_bases
TTGAAAATAGGGGATGTAGTTTTAGAAAATAACGTATTTTTATCACCTATGGCAGGAGTTACAGATAAGCCGTTCAGATT
GATATGTCAGGAAATGGGTGCAGGACTTACTTATACTGAAATGGTAAGCGCTAAAGGGCTTTACTATGGGAGTGAAAATA
CAAAGGCACTTTTGGATATTGACGATGGGGAAAAAGTAGCCATTCAGATATTTGGCTCTGACCCCGAGATTATGGGAGAG
ATTGCAAGAAGGTTGAATGAAAGCAAAGCGTTAATCATTGATATAAACATGGGATGCCCTACTCCAAAGATAGTAAAAAA
TGGAGATGGAGCAGCTTTAATGTTAAAACCGGATTTGGCTGAAAAAGTGATGGAGGCGGTGGTAAAGGCTTCATATAAAC
CAGTAACTGTAAAAATAAGAAAAGGATGGAATGATAATAATGTAAATGCAGTAGAGATTGCACAAATTGCTGAGAGAGTA
GGCATTAAAGCTGTAGCGGTACACGGGAGGACAAGAGAACAGTTTTATTCAGGAAAAGCTGACTGGGAGATAATTAAAAA
AGTCAAGGAAAGCGTAAAGATACCTGTAATAGGCAATGGAGATATATTTACGCCAGAAGATGCAAAAAGAATGCTTGATG
AAACAGGTTGCGATGCCGTAATGATTGGGAGAGGAGCCCAGGGCAATCCGTGGATATTTAAAAGGACTGTCCATTATCTT
AAAACGGGTGAACTTTTGCCTGAACCTACAATAAAGGAAAAAGTAGACCTTATGATAAGGCATCTTACTATGATGGTTGA
GTATAAAGGTGAAAGAACAGGTGTGGTAGAAATGAGAAAGCACATCGCTTGGTATTTAAAAGGCCTTCCAGGCAGTGCAC
GAATGAGAGAAGCAGTATTTAAAATGGAAAGTTATAAAGAAATAAAAGAATTTTTAGAAGGTATTCCTAAAATTTACGAA
AAAGGGTAA

Upstream 100 bases:

>100_bases
AGTAAAGTGAAGGGAGTGGCTTTCCACTCCCTTATTATTTTTGTATCAAAATATGATAGAATATATGAAGGAAACATTCG
TTAAAGGGGTTGAAGCTCTG

Downstream 100 bases:

>100_bases
TAAAAAATCCTGCGAGAGCAGGATTTTTATCTTAAATTTTCAGGCAACGTCTGTCTCATTGGGATGTTTTGCCTATTTAG
AAGGTTTTCTACCAAGTCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKIGDVVLENNVFLSPMAGVTDKPFRLICQEMGAGLTYTEMVSAKGLYYGSENTKALLDIDDGEKVAIQIFGSDPEIMGE
IARRLNESKALIIDINMGCPTPKIVKNGDGAALMLKPDLAEKVMEAVVKASYKPVTVKIRKGWNDNNVNAVEIAQIAERV
GIKAVAVHGRTREQFYSGKADWEIIKKVKESVKIPVIGNGDIFTPEDAKRMLDETGCDAVMIGRGAQGNPWIFKRTVHYL
KTGELLPEPTIKEKVDLMIRHLTMMVEYKGERTGVVEMRKHIAWYLKGLPGSARMREAVFKMESYKEIKEFLEGIPKIYE
KG

Sequences:

>Translated_322_residues
MKIGDVVLENNVFLSPMAGVTDKPFRLICQEMGAGLTYTEMVSAKGLYYGSENTKALLDIDDGEKVAIQIFGSDPEIMGE
IARRLNESKALIIDINMGCPTPKIVKNGDGAALMLKPDLAEKVMEAVVKASYKPVTVKIRKGWNDNNVNAVEIAQIAERV
GIKAVAVHGRTREQFYSGKADWEIIKKVKESVKIPVIGNGDIFTPEDAKRMLDETGCDAVMIGRGAQGNPWIFKRTVHYL
KTGELLPEPTIKEKVDLMIRHLTMMVEYKGERTGVVEMRKHIAWYLKGLPGSARMREAVFKMESYKEIKEFLEGIPKIYE
KG
>Mature_322_residues
MKIGDVVLENNVFLSPMAGVTDKPFRLICQEMGAGLTYTEMVSAKGLYYGSENTKALLDIDDGEKVAIQIFGSDPEIMGE
IARRLNESKALIIDINMGCPTPKIVKNGDGAALMLKPDLAEKVMEAVVKASYKPVTVKIRKGWNDNNVNAVEIAQIAERV
GIKAVAVHGRTREQFYSGKADWEIIKKVKESVKIPVIGNGDIFTPEDAKRMLDETGCDAVMIGRGAQGNPWIFKRTVHYL
KTGELLPEPTIKEKVDLMIRHLTMMVEYKGERTGVVEMRKHIAWYLKGLPGSARMREAVFKMESYKEIKEFLEGIPKIYE
KG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=289, Percent_Identity=31.1418685121107, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=31.1418685121107, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=33.4677419354839, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=34.6491228070175, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=31.25, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=44.4444444444444, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=34.3096234309623, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=29.5138888888889, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=29.9595141700405, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=29.7071129707113, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=28.2407407407407, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=318, Percent_Identity=28.9308176100629, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=26.6447368421053, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=26.5486725663717, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585320, Length=283, Percent_Identity=33.5689045936396, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24580595, Length=249, Percent_Identity=32.5301204819277, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920448, Length=249, Percent_Identity=32.5301204819277, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=31.4285714285714, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921524, Length=281, Percent_Identity=24.5551601423488, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35926; Mature: 35926

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGDVVLENNVFLSPMAGVTDKPFRLICQEMGAGLTYTEMVSAKGLYYGSENTKALLDI
CCCCCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCEEEEEE
DDGEKVAIQIFGSDPEIMGEIARRLNESKALIIDINMGCPTPKIVKNGDGAALMLKPDLA
CCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCEEEECCHHH
EKVMEAVVKASYKPVTVKIRKGWNDNNVNAVEIAQIAERVGIKAVAVHGRTREQFYSGKA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHCCCC
DWEIIKKVKESVKIPVIGNGDIFTPEDAKRMLDETGCDAVMIGRGAQGNPWIFKRTVHYL
HHHHHHHHHHHCEEEEECCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHH
KTGELLPEPTIKEKVDLMIRHLTMMVEYKGERTGVVEMRKHIAWYLKGLPGSARMREAVF
HCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KMESYKEIKEFLEGIPKIYEKG
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGDVVLENNVFLSPMAGVTDKPFRLICQEMGAGLTYTEMVSAKGLYYGSENTKALLDI
CCCCCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCEEEEEE
DDGEKVAIQIFGSDPEIMGEIARRLNESKALIIDINMGCPTPKIVKNGDGAALMLKPDLA
CCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCEEEECCHHH
EKVMEAVVKASYKPVTVKIRKGWNDNNVNAVEIAQIAERVGIKAVAVHGRTREQFYSGKA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHCCCC
DWEIIKKVKESVKIPVIGNGDIFTPEDAKRMLDETGCDAVMIGRGAQGNPWIFKRTVHYL
HHHHHHHHHHHCEEEEECCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHH
KTGELLPEPTIKEKVDLMIRHLTMMVEYKGERTGVVEMRKHIAWYLKGLPGSARMREAVF
HCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KMESYKEIKEFLEGIPKIYEKG
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]