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The map label for this gene is hslU [H]

Identifier: 203284216

GI number: 203284216

Start: 317941

End: 319296

Strand: Reverse

Name: hslU [H]

Synonym: BDU_298

Alternate gene names: 203284216

Gene position: 319296-317941 (Counterclockwise)

Preceding gene: 203284217

Following gene: 203284215

Centisome position: 34.27

GC content: 29.72

Gene sequence:

>1356_bases
TTGATGAATAAGATTGAGGATCAAAGTATAGTACCTAGAGAAATTGTTGCAGAATTGGATAAGTATATAATAGGACAGTC
AGAAGCAAAGAAATTGGTTTCAATTGCTCTTGTTAATAGGTATATAAGGTCTAAACTTCCTAAAGAAATAAGAGACGATG
TTATGCCTAAAAATATTATTATGGTTGGGTCAACTGGAGTTGGGAAAACTGAAATTGCAAGAAGACTTTCAAAGTTTATT
AAGGCTCCTTTTATTAAAGTTGAGGCTACTAAATATACTGAGGTAGGTTATGTTGGTCGTGATGTTGAATCTATGATTCG
TGATCTAATGAGTATTGCAGTTAATATGGTAAGAGAAGAGATGTATGATTCTGTTAAAGAAGAAGCAAGTAAGCGTGCTG
AGGATAGAATCATTGATAAACTTTTAAAAAATTCTGAGAATTCTGAGAATGATAATATTACTGATGAAGAGAAGAGAGTT
CGTGATAAATTGAGAGATAAGTTTAGAAAACAGTTAAGAAAAGGTGAGCTTGATGAGAATCTTATCGATGTTTATGTTTC
GGGTAAGATGCCTGTTTCTACTATAGAAATATTTTCAGGTGGTAATTTAGAAGAGATTGATATGAGTATTGGGGGTCTTA
TTAATAATATCTTTGATAGAAAAAAGAGAAGAGAGTTAAAAATAAAAAAAGCTAGAGAGATAATTATATCTGAGGAGCTT
GAAAAATTAGTTGATTATGAAAATATTGTAGATATTGCTAAATCACGGGTTGAAAATATGGGTATTGTTTTTATTGATGA
GATAGATAAAATAGTTACTAAAAATAGAACTGGAAATGATGTATCTAGAGAAGGTGTGCAGAGAGATATTTTGCCAATTG
TTGAAGGATCTAAAGTTAATACTAGGTATGGAATAGTTGATACTTCTCATATTTTGTTCATTGCTGCTGGTGCATTTAAT
TTATCTAAACCATCTGATTTAATACCAGAGCTTCAAGGTAGGTTTCCAATTAAAGTTGAACTTACAAGTTTGAGTATAGA
TGATTTTAAAAATATTTTAAAGTGTACTAAAAATTCTTTGATAAAACAGTACATTGCGATGTTTAAAGTTTATAATTTAA
CCTTAACATTTACTGAAGAGGCAATTGATAGGATTGCTGAACTTACATTTAATATGAATTTTGAGGGAGAAAATCTTGGT
GCACGGAGATTGCATGGAGTTATGGAAAAAATCCTTGCCGATCTTTTTTTTGAAGCACCTGGTAGTAAGTTGAAAAAAAT
TGAAATAAATTTGGACTATGTTGATGAAAAAATAAAAATTAACGAACAAAAAGACTTAAATTATTATATAATATAG

Upstream 100 bases:

>100_bases
ATAAAAAATTAAGTGCTGCTGATATTGCTTTTAAAGCTTTAAAGATAGCAGCAAGAGTTTGTATATATACAAATTCAAAT
ATTGTGCTTGAGGAGATTGG

Downstream 100 bases:

>100_bases
TAAAAAACAATTTGTAGTATGAGGATATTAGTTTGGATATTTTTCAAAGATCAGTAGATTTGACACATAGGTATTTAGAT
GTTCTTGGGTTAAGACAAAG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMNKIEDQSIVPREIVAELDKYIIGQSEAKKLVSIALVNRYIRSKLPKEIRDDVMPKNIIMVGSTGVGKTEIARRLSKFI
KAPFIKVEATKYTEVGYVGRDVESMIRDLMSIAVNMVREEMYDSVKEEASKRAEDRIIDKLLKNSENSENDNITDEEKRV
RDKLRDKFRKQLRKGELDENLIDVYVSGKMPVSTIEIFSGGNLEEIDMSIGGLINNIFDRKKRRELKIKKAREIIISEEL
EKLVDYENIVDIAKSRVENMGIVFIDEIDKIVTKNRTGNDVSREGVQRDILPIVEGSKVNTRYGIVDTSHILFIAAGAFN
LSKPSDLIPELQGRFPIKVELTSLSIDDFKNILKCTKNSLIKQYIAMFKVYNLTLTFTEEAIDRIAELTFNMNFEGENLG
ARRLHGVMEKILADLFFEAPGSKLKKIEINLDYVDEKIKINEQKDLNYYII

Sequences:

>Translated_451_residues
MMNKIEDQSIVPREIVAELDKYIIGQSEAKKLVSIALVNRYIRSKLPKEIRDDVMPKNIIMVGSTGVGKTEIARRLSKFI
KAPFIKVEATKYTEVGYVGRDVESMIRDLMSIAVNMVREEMYDSVKEEASKRAEDRIIDKLLKNSENSENDNITDEEKRV
RDKLRDKFRKQLRKGELDENLIDVYVSGKMPVSTIEIFSGGNLEEIDMSIGGLINNIFDRKKRRELKIKKAREIIISEEL
EKLVDYENIVDIAKSRVENMGIVFIDEIDKIVTKNRTGNDVSREGVQRDILPIVEGSKVNTRYGIVDTSHILFIAAGAFN
LSKPSDLIPELQGRFPIKVELTSLSIDDFKNILKCTKNSLIKQYIAMFKVYNLTLTFTEEAIDRIAELTFNMNFEGENLG
ARRLHGVMEKILADLFFEAPGSKLKKIEINLDYVDEKIKINEQKDLNYYII
>Mature_451_residues
MMNKIEDQSIVPREIVAELDKYIIGQSEAKKLVSIALVNRYIRSKLPKEIRDDVMPKNIIMVGSTGVGKTEIARRLSKFI
KAPFIKVEATKYTEVGYVGRDVESMIRDLMSIAVNMVREEMYDSVKEEASKRAEDRIIDKLLKNSENSENDNITDEEKRV
RDKLRDKFRKQLRKGELDENLIDVYVSGKMPVSTIEIFSGGNLEEIDMSIGGLINNIFDRKKRRELKIKKAREIIISEEL
EKLVDYENIVDIAKSRVENMGIVFIDEIDKIVTKNRTGNDVSREGVQRDILPIVEGSKVNTRYGIVDTSHILFIAAGAFN
LSKPSDLIPELQGRFPIKVELTSLSIDDFKNILKCTKNSLIKQYIAMFKVYNLTLTFTEEAIDRIAELTFNMNFEGENLG
ARRLHGVMEKILADLFFEAPGSKLKKIEINLDYVDEKIKINEQKDLNYYII

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=36.036036036036, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790366, Length=445, Percent_Identity=46.9662921348315, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786642, Length=243, Percent_Identity=34.156378600823, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=35.4609929078014, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648291, Length=241, Percent_Identity=30.7053941908714, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648289, Length=241, Percent_Identity=30.7053941908714, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR011704
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07724 AAA_2; PF07728 AAA_5; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 51749; Mature: 51749

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNKIEDQSIVPREIVAELDKYIIGQSEAKKLVSIALVNRYIRSKLPKEIRDDVMPKNII
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEE
MVGSTGVGKTEIARRLSKFIKAPFIKVEATKYTEVGYVGRDVESMIRDLMSIAVNMVREE
EEECCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MYDSVKEEASKRAEDRIIDKLLKNSENSENDNITDEEKRVRDKLRDKFRKQLRKGELDEN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
LIDVYVSGKMPVSTIEIFSGGNLEEIDMSIGGLINNIFDRKKRRELKIKKAREIIISEEL
EEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLVDYENIVDIAKSRVENMGIVFIDEIDKIVTKNRTGNDVSREGVQRDILPIVEGSKVN
HHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
TRYGIVDTSHILFIAAGAFNLSKPSDLIPELQGRFPIKVELTSLSIDDFKNILKCTKNSL
CEECCEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
IKQYIAMFKVYNLTLTFTEEAIDRIAELTFNMNFEGENLGARRLHGVMEKILADLFFEAP
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GSKLKKIEINLDYVDEKIKINEQKDLNYYII
CCCEEEEEEEEEECCCEEEECCCCCCCEEEC
>Mature Secondary Structure
MMNKIEDQSIVPREIVAELDKYIIGQSEAKKLVSIALVNRYIRSKLPKEIRDDVMPKNII
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEE
MVGSTGVGKTEIARRLSKFIKAPFIKVEATKYTEVGYVGRDVESMIRDLMSIAVNMVREE
EEECCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MYDSVKEEASKRAEDRIIDKLLKNSENSENDNITDEEKRVRDKLRDKFRKQLRKGELDEN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
LIDVYVSGKMPVSTIEIFSGGNLEEIDMSIGGLINNIFDRKKRRELKIKKAREIIISEEL
EEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLVDYENIVDIAKSRVENMGIVFIDEIDKIVTKNRTGNDVSREGVQRDILPIVEGSKVN
HHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
TRYGIVDTSHILFIAAGAFNLSKPSDLIPELQGRFPIKVELTSLSIDDFKNILKCTKNSL
CEECCEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
IKQYIAMFKVYNLTLTFTEEAIDRIAELTFNMNFEGENLGARRLHGVMEKILADLFFEAP
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GSKLKKIEINLDYVDEKIKINEQKDLNYYII
CCCEEEEEEEEEECCCEEEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]