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The map label for this gene is IlvD

Identifier: 20094633

GI number: 20094633

Start: 1190631

End: 1192280

Strand: Reverse

Name: IlvD

Synonym: MK1197

Alternate gene names: 20094633

Gene position: 1192280-1190631 (Counterclockwise)

Preceding gene: 20094637

Following gene: 20094632

Centisome position: 70.34

GC content: 67.03

Gene sequence:

>1650_bases
TTGCGCAGCGGTGATATCAAGGAGGGCGTCGAACGGACGCCGCACCGGGCGCTGCTTCGCGCGTGCGGACTCACTGACGA
GGAGATGGACCGACCGTTCGTCGCGGTGGTGAACACGTACTCGGAGGTCGTTCCGGGACACATGCACCTGGACAAGGTGA
CGGAGGCCGTCAAGGCCGGGATCCGGATGGCCGGCGGGGTGCCGTTCGAGGTCGAGACGATCGCCCTGTGCGACGGTATT
GCGATGAACACGCCCGGTATGAAATATTCCCTCCCGAGCCGTGAGCTCGTGGCCGATACTATCGAGACCGTAATCGAGGC
GCACCGGTTCGACGGGTTCGTGGCGATCGTCTCGTGTGATAAGATGGTGCCGGGGGCACTGATGGCGGCAGCGCGGCTGG
ACCTACCCGCGGCGATCGTGACCGGAGGCCCGATGGAGCCCGGGTGCGTGGACGGCGAGCGGGTGGACTTGATCGACGCG
TTCGAGGCGGTGGGAGCGTACGAGGAGGGGGAGATTTCGGAGGAAGAACTCGAGGAGCTGGAGCAGCGCGCGTGTCCGGG
ACCGGGATCCTGCGCCGGGATGTTCACGGCGAACACGATGGCGTGCATGACCGAGGTGCTGGGGATGTCGGAGTTCAACT
GCGCGGCGACCCCGGCGACGGAGGCGGAGAAGCTGCGGGTCGCGAAGCTTACGGGTATGCGGATCGTGGAGGCGATCGAG
GAAGGGATCACCGCGCGGGACGTACTCACGCGGGAGGCCTTCCTGGACGCGATCCGTGTCGACATGGCGCTGGGCGGGAG
CACGAACACCGTGCTGCACCTGCTCGCGATCGCGCGTGAGGCCGACGTTGAGCTTTCCCTCGACGACTTCGACGAGCTGA
GCCGGGAGACGCCGCATCTGTGCGCGATGCGTCCGGGCGGTCCCTACACGATGCGGGACCTGTACGAGGCGGGTGGCGTT
CCCGCGGTCATGAAGGAACTGGCGGACGACCTGCACCTGGACAGGATCGACTTCGCGGGTCGATCGATGCGGGAACGGGT
CGAACGGACCGAGGTAAAGGATCGCGAGGTGATCCGGCCGAAGGAGGATCCCGTGCACGAGGAAGGTGGTATCGTCGTCC
TGTACGGGAACCTCGCCCCGAAGGGTGCGGTGATCAAGACGGCGGCGCTCTCGGAGGAGATGTATGAGCACGAGGGTCCG
GCGGTGGTGTTCGACTCGGAGGAGGAGGCGACGGAGGCGATACTGGGTGGCGATATCGACCCGGGTGACGTGGTAGTGAT
CCGATACGAAGGCCCCGCCGGCGGTCCCGGAATGCGTGAGATGCTCACACCTACGGCGGCGCTGTGCGGAATGGGACTGG
ACGACTCCGTGGCGCTCGTGACGGACGGGCGGTTCTCCGGCGGTACGCGGGGTCCGTGCGTGGGACACGTGTCACCGGAG
GCGTACCGGGGCGGACCGATCGCGGTCGTGGAGGAAGGAGACACGATCCGGCTCGACGTGCGGGAGCGACGGCTCGAGGT
GGACGTCGAGGACGAGGAGCTGGAGGCCAGGTTGGAGGAGTGGGAGCCCCCGGAGGACGAGGTCACGGGGTACTTGAGGA
GGTACCGAGAGCTCGTCCGCGGGGCCGACGAAGGGGCCGTACTGCGGTGA

Upstream 100 bases:

>100_bases
CTCGATCACGCCCTTGAGCACGCCGTACGGGTCCAAGGCCTCCAACCCCAGCCATAAGCGTTTAACCTACGCGCCCCGGG
GTCGGCCGGGGGGTTTCCGG

Downstream 100 bases:

>100_bases
CCCTCGGGGAGCGGACGGGTCGGGGCCGTTCGATCGCCGAGCGCAGCCTCGAGCGGTGGACCGAGAAGCTCGGTGAGCCC
GAGAACTTAGAGGACCTCCT

Product: dihydroxyacid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRSGDIKEGVERTPHRALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAGIRMAGGVPFEVETIALCDGI
AMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCDKMVPGALMAAARLDLPAAIVTGGPMEPGCVDGERVDLIDA
FEAVGAYEEGEISEEELEELEQRACPGPGSCAGMFTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIE
EGITARDVLTREAFLDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHLCAMRPGGPYTMRDLYEAGGV
PAVMKELADDLHLDRIDFAGRSMRERVERTEVKDREVIRPKEDPVHEEGGIVVLYGNLAPKGAVIKTAALSEEMYEHEGP
AVVFDSEEEATEAILGGDIDPGDVVVIRYEGPAGGPGMREMLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPE
AYRGGPIAVVEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPPEDEVTGYLRRYRELVRGADEGAVLR

Sequences:

>Translated_549_residues
MRSGDIKEGVERTPHRALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAGIRMAGGVPFEVETIALCDGI
AMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCDKMVPGALMAAARLDLPAAIVTGGPMEPGCVDGERVDLIDA
FEAVGAYEEGEISEEELEELEQRACPGPGSCAGMFTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIE
EGITARDVLTREAFLDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHLCAMRPGGPYTMRDLYEAGGV
PAVMKELADDLHLDRIDFAGRSMRERVERTEVKDREVIRPKEDPVHEEGGIVVLYGNLAPKGAVIKTAALSEEMYEHEGP
AVVFDSEEEATEAILGGDIDPGDVVVIRYEGPAGGPGMREMLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPE
AYRGGPIAVVEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPPEDEVTGYLRRYRELVRGADEGAVLR
>Mature_549_residues
MRSGDIKEGVERTPHRALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAGIRMAGGVPFEVETIALCDGI
AMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCDKMVPGALMAAARLDLPAAIVTGGPMEPGCVDGERVDLIDA
FEAVGAYEEGEISEEELEELEQRACPGPGSCAGMFTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIE
EGITARDVLTREAFLDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHLCAMRPGGPYTMRDLYEAGGV
PAVMKELADDLHLDRIDFAGRSMRERVERTEVKDREVIRPKEDPVHEEGGIVVLYGNLAPKGAVIKTAALSEEMYEHEGP
AVVFDSEEEATEAILGGDIDPGDVVVIRYEGPAGGPGMREMLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPE
AYRGGPIAVVEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPPEDEVTGYLRRYRELVRGADEGAVLR

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=604, Percent_Identity=41.7218543046358, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788157, Length=600, Percent_Identity=32, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1786464, Length=500, Percent_Identity=33.6, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI2367371, Length=559, Percent_Identity=30.5903398926655, Blast_Score=217, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322476, Length=546, Percent_Identity=42.3076923076923, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_METKA (Q8TW40)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614480.1
- ProteinModelPortal:   Q8TW40
- SMR:   Q8TW40
- GeneID:   1477298
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1197
- NMPDR:   fig|190192.1.peg.1193
- HOGENOM:   HBG671001
- OMA:   NSHNEII
- BioCyc:   MKAN190192:MK1197-MONOMER
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59377; Mature: 59377

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSGDIKEGVERTPHRALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAG
CCCCCHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IRMAGGVPFEVETIALCDGIAMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCD
HHHCCCCCEEEEEEEEECCCEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KMVPGALMAAARLDLPAAIVTGGPMEPGCVDGERVDLIDAFEAVGAYEEGEISEEELEEL
CCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EQRACPGPGSCAGMFTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIE
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHH
EGITARDVLTREAFLDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHL
HCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCE
CAMRPGGPYTMRDLYEAGGVPAVMKELADDLHLDRIDFAGRSMRERVERTEVKDREVIRP
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCHHCCCC
KEDPVHEEGGIVVLYGNLAPKGAVIKTAALSEEMYEHEGPAVVFDSEEEATEAILGGDID
CCCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCC
PGDVVVIRYEGPAGGPGMREMLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPE
CCCEEEEEECCCCCCCCHHHHHCHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCCH
AYRGGPIAVVEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPPEDEVTGYLRRYRELVR
HHCCCCEEEEECCCEEEEEEECCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GADEGAVLR
CCCCCCCCC
>Mature Secondary Structure
MRSGDIKEGVERTPHRALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAG
CCCCCHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IRMAGGVPFEVETIALCDGIAMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCD
HHHCCCCCEEEEEEEEECCCEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KMVPGALMAAARLDLPAAIVTGGPMEPGCVDGERVDLIDAFEAVGAYEEGEISEEELEEL
CCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EQRACPGPGSCAGMFTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIE
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHH
EGITARDVLTREAFLDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHL
HCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCE
CAMRPGGPYTMRDLYEAGGVPAVMKELADDLHLDRIDFAGRSMRERVERTEVKDREVIRP
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCHHCCCC
KEDPVHEEGGIVVLYGNLAPKGAVIKTAALSEEMYEHEGPAVVFDSEEEATEAILGGDID
CCCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCC
PGDVVVIRYEGPAGGPGMREMLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPE
CCCEEEEEECCCCCCCCHHHHHCHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCCH
AYRGGPIAVVEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPPEDEVTGYLRRYRELVR
HHCCCCEEEEECCCEEEEEEECCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GADEGAVLR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014