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The map label for this gene is CobF [H]

Identifier: 20094474

GI number: 20094474

Start: 999865

End: 1000527

Strand: Direct

Name: CobF [H]

Synonym: MK1038

Alternate gene names: 20094474

Gene position: 999865-1000527 (Clockwise)

Preceding gene: 20094471

Following gene: 20094475

Centisome position: 58.99

GC content: 63.35

Gene sequence:

>663_bases
TTGAACACGGGAGCACCCACCCTGTACGGAGTGGGCCTGGGCCCCGGGGATCCCGACCTGCTAACTCGTAAGGCGATCAG
GATTATAAAGAAGGTACCCGTCGCGATGGTACCCTTCGTGGGCGCGGAGAGTCGAGCCGCCAGGGTAGTGCGCGCCGTCG
ACCCGGAGGCCACCGTGGTCGGTTACCACGCACCGATGACCAGGGACCCGGAGGAACGCGATCGGGCGTACTCGAAGGCC
GCGGAAACCTTGGCGGAACTGCTCGAGCGGTTTCACGAAGTGGCGGTCTGCAACCTCGGAGATCCCACCCTCTACGCCAC
GTTCTGGCACATCGTGGAGCGGATCCCACTAGAGGACTTCGAGATAGAGCTGGTCCCCGGAATCCCGGCGGGACTCCTCT
GTGCGGCTCGCATCGGCCGCCCCTTAGCGATGGAGAGTGACAAGGTCCTAATCTGCACTCGGGAACCGCCAGAAGACCTC
GAAGGCATGGATACGGTGATACTCTATAAACCAACCCGGCGAGGCGTCGAAAGGCTTCTCAGGGAGGGGTTCGAGGTCTA
CGCGTGTCGGGAGCTGGGGTTCGAGGGAGAGCGAGTGGAGCGCGTAAACGGGGCCGAGTTCGAGCCCAGTTACCTGTGCA
CGATAGTCGCCCTCCGGCCCTGA

Upstream 100 bases:

>100_bases
GCCCGGTCGCGGTCGAAGTACGGCCAGCACCGTCGCTTTCATCACCGGCTTGGTGGGGCCTGGAACACCCTCTGTGACCA
AGTCGGAGGGGTCTACGACA

Downstream 100 bases:

>100_bases
GATACCAGGGCGGGGGCCGGGTTTTTACCCGGTGACTACCCGGGAGGTTCGGGGGCGAGGGTTGCTCCGTACAGTGTGGG
TAGATTACGCCAGGAAAGGC

Product: precorrin-2 methylase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVVGYHAPMTRDPEERDRAYSKA
AETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDFEIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDL
EGMDTVILYKPTRRGVERLLREGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP

Sequences:

>Translated_220_residues
MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVVGYHAPMTRDPEERDRAYSKA
AETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDFEIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDL
EGMDTVILYKPTRRGVERLLREGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP
>Mature_220_residues
MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVVGYHAPMTRDPEERDRAYSKA
AETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDFEIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDL
EGMDTVILYKPTRRGVERLLREGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 24389; Mature: 24389

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHCCCCCEEE
GYHAPMTRDPEERDRAYSKAAETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCC
EIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDLEGMDTVILYKPTRRGVERLL
EEEEECCCCHHHHHHHHHCCCEEECCCCEEEEECCCCHHHCCCCEEEEECCCHHHHHHHH
REGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP
HCCHHEEEHHHCCCCCCHHHHCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHCCCCCEEE
GYHAPMTRDPEERDRAYSKAAETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCC
EIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDLEGMDTVILYKPTRRGVERLL
EEEEECCCCHHHHHHHHHCCCEEECCCCEEEEECCCCHHHCCCCEEEEECCCHHHHHHHH
REGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP
HCCHHEEEHHHCCCCCCHHHHCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]