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The map label for this gene is KsgA

Identifier: 20094370

GI number: 20094370

Start: 893506

End: 894342

Strand: Reverse

Name: KsgA

Synonym: MK0934

Alternate gene names: 20094370

Gene position: 894342-893506 (Counterclockwise)

Preceding gene: 20094371

Following gene: 20094369

Centisome position: 52.76

GC content: 62.01

Gene sequence:

>837_bases
TTGAGAAGCGTTGAGTACCTCGCGTACCTGCGATCCAAGTACGGGATCCGTCCCAGGCGACGCTTGGGCCAGCACTTCAT
GGTAGACGATAATATCCTGGAGTTCATGGTGGAGGCGGCGGAGGTACGCGAGGACGACATAGTGTTAGAAATAGGCCCCG
GACCCGGTCTCCTGACCCGTTACCTGATGACCCGAGCGGGTCAAGTGATCGCCGTCGAGCTCGACGGACGCATGGTGGAG
ATTCTAAAGCGGGAGCTCGGTGAGGCCCCCAACCTCGAGATCGTACGGGCCGACTTCTTGGAGTACGACGTTCCGGACGA
CGTGAACAAGGTCGTAGCCAACATCCCCTACAACATCTCGTCTCCGATCACGTTCAAGCTCCTGGAGCTGGACATCGACG
TCGCGGTGTTGACCTACCAACGGGAGTTCGCCGAGCGAATGGTGGCCGAACCCGGATCCAAGAAGTACAGCCGCCTCACC
GTGATGGTGAACCTGCTCGCGGACGTGGAGCTCCTCAGAGGGGTACCCCGCAGGGCCTTCATCCCTCCCCCGCGCGTGGG
CTCCTCCGTGGTCCGCCTGACGCCCAAGTCGGAGGAGGAGCGCCCCGATGTGGATCCGGACACCTTGGAATCCGTGTGTC
GAGCCCTCTTCCAGCACAAGAACAAAACGGTGAGGAACGCGTTACTCCTCTCCGCTCACGAGTGGGCGACCGATCGCGAG
CAGGCTCGAGAGGTACTGGAAGAGCTCCCGGAGGACCTACTATCCGAGCGTCCGCTCCATCTACCTCCGGAGCGTGTGGC
GGAGCTCGCCGCCGCTATCGAGAGCGCACTGGGGTAG

Upstream 100 bases:

>100_bases
ATAGGGGACGTACTTACATCCGTCGGTCGCGAGGTTAACTTGAACGACCCGAGAACAGTCGTCACCGTAGACGTCCTGGG
ACCGAGGGTGTCCGTCGGCG

Downstream 100 bases:

>100_bases
GTGGATGAAGCTCAAGATCTGGTCACCGGTCCGGTTGTTCGGGGCTACTCCGGGTGCGGTCGTGGGTATCTCTCTGACGG
ACATCGAGGTCCTGTTCGAT

Product: dimethyladenosine transferase (rRNA methylase)

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTRYLMTRAGQVIAVELDGRMVE
ILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNISSPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLT
VMVNLLADVELLRGVPRRAFIPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDRE
QAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG

Sequences:

>Translated_278_residues
MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTRYLMTRAGQVIAVELDGRMVE
ILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNISSPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLT
VMVNLLADVELLRGVPRRAFIPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDRE
QAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG
>Mature_278_residues
MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTRYLMTRAGQVIAVELDGRMVE
ILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNISSPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLT
VMVNLLADVELLRGVPRRAFIPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDRE
QAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=228, Percent_Identity=41.2280701754386, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI156415992, Length=239, Percent_Identity=30.9623430962343, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786236, Length=226, Percent_Identity=33.6283185840708, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=215, Percent_Identity=42.7906976744186, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25141369, Length=279, Percent_Identity=28.3154121863799, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=208, Percent_Identity=39.4230769230769, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21358017, Length=218, Percent_Identity=42.2018348623853, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21357273, Length=282, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METKA (Q8TWU7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614217.1
- ProteinModelPortal:   Q8TWU7
- GeneID:   1477035
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0934
- NMPDR:   fig|190192.1.peg.930
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- BioCyc:   MKAN190192:MK0934-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31521; Mature: 31521

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 25-25 BINDING 27-27 BINDING 52-52 BINDING 73-73 BINDING 98-98 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTR
CCHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
YLMTRAGQVIAVELDGRMVEILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNIS
HHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHCCCCCC
SPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLTVMVNLLADVELLRGVPRRAF
CCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDRE
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
QAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTR
CCHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
YLMTRAGQVIAVELDGRMVEILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNIS
HHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHCCCCCC
SPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLTVMVNLLADVELLRGVPRRAF
CCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDRE
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
QAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014