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The map label for this gene is top6B [H]

Identifier: 20094357

GI number: 20094357

Start: 878332

End: 879759

Strand: Direct

Name: top6B [H]

Synonym: MK0921

Alternate gene names: 20094357

Gene position: 878332-879759 (Clockwise)

Preceding gene: 20094356

Following gene: 20094358

Centisome position: 51.82

GC content: 65.62

Gene sequence:

>1428_bases
ATGGAGCGGCTGGAATGGCGTGAATCCTCACCGGCGGAGTTCTTCGAGCGGAACCGCGAGATGCTCGGGTTCGACGGACC
CATCAAGAGCATGGTGATGACCGTCCACGAGCTCGTCACCAACTCGATGGACGCGTGCCACCTGAACCGCATACGTCCCG
ACGTGCGGGTGGTGATCCGACGCGAGGAGGAGAACGTGTATCGGGTGCGAGTGATCGACAACGGGCCGGGGATCCCGCCG
GAGCGCGTGCCGAAGGTGTTCGGTAAGTTCCTCGCGGGAGATAAGTTCGACCCCGTCTACGGGATCCAGTCGATGGGCCA
GCAGGGGATCGGAGCCGCCGGCGTCGCCCTCTACGCCCTGATCACCACGGGCGAACCCGTGCGCGTGCTCACATCCACCG
ACGGTAGAACCGCGCACTACTTCGAGGTCAAGCCCGATCCCTCGACCAACGAGCCGGTGGTCGTCCGGCGGGAGAAGCGA
CCCGCGAGCCGCCGCGGGACGACGGTGGAGGTGACCATCGGGGACGCCGTCTACGAGTCGGGTCGCCGCGGTCCCCGGGA
GTACCTTCGTAGGCTGCACGCGGTGAACCCCCACGCCCGGATCTCCCTCCGCGACCCCGACGGTAACGCGCACGTCTGGG
AGCCGCTGGTCGAGGAACTCCCCGACCCACCTCGAGTGCTGAAGCCTCATCCTCACGCCCTCGACGCCCACAAGCTCCTC
CGGATCGCCGAGCGGACGTCCCGGAGGACCGTCCGAACGATGCTCGTCGGGGAGCTCTGCAGGTTCTCGGAGGCCCGGGT
CGAAGAGCTCCGGGAGCGACTGGCGGGCCGTGTGGATCTGGAGAAGGACCCGAAGGAGTTGACCCGCGAGGAGGCCGAGC
TCATCGTGAAGGCGCTGCACGAGATGGACTTCATGCGCCCGCCGTCCGACGTCGTTTCCCCGGTCGGAGAGTCGGCGCTG
AAGGCCGCGCTGAAATCGGAGGGAGCTCGACTGGTCTCGGCGGTTTCTCGGCGGCCGCTCGTACTGCGCGATAACGTGAT
CCAGGTCGAGGTGGCCGTGGGTTACGGGGAGGAGGGGGATCTGTGGCGGTTCGCGAACAGGGCTCCGTTGATGTTCAGGG
CGGGCGGTTGTTCCATCACCAAGGGCGTCGAGGAGGTGGATTTCGGCCGGTACGGTCTCGAGGAGGATCGGTTACTGATC
CTGGTGAACGTGAACTCCCCGTTCGTTCCGTTCAGCGGGCCCGCTAAGCAGGAGATCGGTAGCGAATACGTCGAAGAGGA
AGTAAAGCGGGCGGTACAGCGGGCGTGTCGGGAGCTGGGTCGTGAGGTCCGACGGCGACGTCGGGCCCGTCTCGAGCGCC
AGAAGATGAAAAGGATCGATAGGAACCTGAGAATCGTGAGGAAGAAGGCCATCGAGATACTCTCGTGA

Upstream 100 bases:

>100_bases
GTCTTCGGAGAAGCGCGAGATCGAGAGACCGCCATCAGGCGGGTGGAGCACTGGAAGGAACGCGTCGAGGAGATCGTCTC
GGAGCTGAAGGGGTAGCTGG

Downstream 100 bases:

>100_bases
TGAGCTCACGACGGGGAGACCTAGTCGGGATGATCGACCCCTAGCAGGCTGAAACGGCGCGGGTGGAGGTAGCCGGCGGG
GAGCGCGGGCTTCAGACCCG

Product: DNA topoisomerase VI subunit B

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit B; TopoVI-B [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MERLEWRESSPAEFFERNREMLGFDGPIKSMVMTVHELVTNSMDACHLNRIRPDVRVVIRREEENVYRVRVIDNGPGIPP
ERVPKVFGKFLAGDKFDPVYGIQSMGQQGIGAAGVALYALITTGEPVRVLTSTDGRTAHYFEVKPDPSTNEPVVVRREKR
PASRRGTTVEVTIGDAVYESGRRGPREYLRRLHAVNPHARISLRDPDGNAHVWEPLVEELPDPPRVLKPHPHALDAHKLL
RIAERTSRRTVRTMLVGELCRFSEARVEELRERLAGRVDLEKDPKELTREEAELIVKALHEMDFMRPPSDVVSPVGESAL
KAALKSEGARLVSAVSRRPLVLRDNVIQVEVAVGYGEEGDLWRFANRAPLMFRAGGCSITKGVEEVDFGRYGLEEDRLLI
LVNVNSPFVPFSGPAKQEIGSEYVEEEVKRAVQRACRELGREVRRRRRARLERQKMKRIDRNLRIVRKKAIEILS

Sequences:

>Translated_475_residues
MERLEWRESSPAEFFERNREMLGFDGPIKSMVMTVHELVTNSMDACHLNRIRPDVRVVIRREEENVYRVRVIDNGPGIPP
ERVPKVFGKFLAGDKFDPVYGIQSMGQQGIGAAGVALYALITTGEPVRVLTSTDGRTAHYFEVKPDPSTNEPVVVRREKR
PASRRGTTVEVTIGDAVYESGRRGPREYLRRLHAVNPHARISLRDPDGNAHVWEPLVEELPDPPRVLKPHPHALDAHKLL
RIAERTSRRTVRTMLVGELCRFSEARVEELRERLAGRVDLEKDPKELTREEAELIVKALHEMDFMRPPSDVVSPVGESAL
KAALKSEGARLVSAVSRRPLVLRDNVIQVEVAVGYGEEGDLWRFANRAPLMFRAGGCSITKGVEEVDFGRYGLEEDRLLI
LVNVNSPFVPFSGPAKQEIGSEYVEEEVKRAVQRACRELGREVRRRRRARLERQKMKRIDRNLRIVRKKAIEILS
>Mature_475_residues
MERLEWRESSPAEFFERNREMLGFDGPIKSMVMTVHELVTNSMDACHLNRIRPDVRVVIRREEENVYRVRVIDNGPGIPP
ERVPKVFGKFLAGDKFDPVYGIQSMGQQGIGAAGVALYALITTGEPVRVLTSTDGRTAHYFEVKPDPSTNEPVVVRREKR
PASRRGTTVEVTIGDAVYESGRRGPREYLRRLHAVNPHARISLRDPDGNAHVWEPLVEELPDPPRVLKPHPHALDAHKLL
RIAERTSRRTVRTMLVGELCRFSEARVEELRERLAGRVDLEKDPKELTREEAELIVKALHEMDFMRPPSDVVSPVGESAL
KAALKSEGARLVSAVSRRPLVLRDNVIQVEVAVGYGEEGDLWRFANRAPLMFRAGGCSITKGVEEVDFGRYGLEEDRLLI
LVNVNSPFVPFSGPAKQEIGSEYVEEEVKRAVQRACRELGREVRRRRRARLERQKMKRIDRNLRIVRKKAIEILS

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP [H]

COG id: COG1389

COG function: function code L; DNA topoisomerase VI, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR010979
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005734
- InterPro:   IPR015320
- ProDom:   PD013790 [H]

Pfam domain/function: PF02518 HATPase_c; PF09239 Topo-VIb_trans [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 54010; Mature: 54010

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLEWRESSPAEFFERNREMLGFDGPIKSMVMTVHELVTNSMDACHLNRIRPDVRVVIR
CCCCCCCCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEE
REEENVYRVRVIDNGPGIPPERVPKVFGKFLAGDKFDPVYGIQSMGQQGIGAAGVALYAL
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
ITTGEPVRVLTSTDGRTAHYFEVKPDPSTNEPVVVRREKRPASRRGTTVEVTIGDAVYES
EECCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHC
GRRGPREYLRRLHAVNPHARISLRDPDGNAHVWEPLVEELPDPPRVLKPHPHALDAHKLL
CCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RIAERTSRRTVRTMLVGELCRFSEARVEELRERLAGRVDLEKDPKELTREEAELIVKALH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
EMDFMRPPSDVVSPVGESALKAALKSEGARLVSAVSRRPLVLRDNVIQVEVAVGYGEEGD
HHHCCCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHCCCEEEECCEEEEEEEECCCCCCC
LWRFANRAPLMFRAGGCSITKGVEEVDFGRYGLEEDRLLILVNVNSPFVPFSGPAKQEIG
CCHHCCCCCEEEECCCCCHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHH
SEYVEEEVKRAVQRACRELGREVRRRRRARLERQKMKRIDRNLRIVRKKAIEILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERLEWRESSPAEFFERNREMLGFDGPIKSMVMTVHELVTNSMDACHLNRIRPDVRVVIR
CCCCCCCCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEE
REEENVYRVRVIDNGPGIPPERVPKVFGKFLAGDKFDPVYGIQSMGQQGIGAAGVALYAL
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
ITTGEPVRVLTSTDGRTAHYFEVKPDPSTNEPVVVRREKRPASRRGTTVEVTIGDAVYES
EECCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHC
GRRGPREYLRRLHAVNPHARISLRDPDGNAHVWEPLVEELPDPPRVLKPHPHALDAHKLL
CCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RIAERTSRRTVRTMLVGELCRFSEARVEELRERLAGRVDLEKDPKELTREEAELIVKALH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
EMDFMRPPSDVVSPVGESALKAALKSEGARLVSAVSRRPLVLRDNVIQVEVAVGYGEEGD
HHHCCCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHCCCEEEECCEEEEEEEECCCCCCC
LWRFANRAPLMFRAGGCSITKGVEEVDFGRYGLEEDRLLILVNVNSPFVPFSGPAKQEIG
CCHHCCCCCEEEECCCCCHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHH
SEYVEEEVKRAVQRACRELGREVRRRRRARLERQKMKRIDRNLRIVRKKAIEILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]