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The map label for this gene is AroA

Identifier: 20094066

GI number: 20094066

Start: 590310

End: 591596

Strand: Direct

Name: AroA

Synonym: MK0628

Alternate gene names: 20094066

Gene position: 590310-591596 (Clockwise)

Preceding gene: 20094064

Following gene: 20094069

Centisome position: 34.83

GC content: 68.76

Gene sequence:

>1287_bases
GTGAAGAGGGTCGAGCTGGAGGGTATCCCGGAGGTTCGGGGGACCGTCTGTCCGCCTCCCTCGAAGAGCGGGTCCCACCG
GGCCCTGATCGCCGCCTCGTTATGCGACGGGAGTACCGAGCTCTGGAACGTGCTGGACGCGGAAGACGTGCGCGCGACGC
TTCGCCTATGTCGGATGCTGGGGGCGGAGGTGGACGTCGACGGTGAGGAGCGGCTCGAGGCGACGGTCTCCGGGTTCGGG
GATTCCCCGCGTGCGCCCGAGGACGTCGTGGACTGCGGGAACTCGGGGACTACGTTAAGGTTGGGGTGCGGGCTGGCCGC
CCTCGTCGAGGGCACCACAATCCTGACGGGGGACGACTCCCTCAGGTCCCGTCCCGTCGGGGACCTCCTGGCGGCGCTCC
GCTCCCTGGGTGTGGACGCCCGCGGAAGGGTGGTGCGGGGTGAGGAGTACCCCCCGGTGGTGATCTCGGGGCGCCCGCTC
CGGGAACGCGTCGCGGTGTACGGGGACGTCAGCTCCCAGTTCGTGAGCGCGCTCCTCTTTCTGGGTGCCGGGCTCGGTGC
GCTCCGCGTGGACGTGGTGGGGGACCTGCGCTCTCGCCCCTACGTGGACATGACCGTCGAGACGCTGGAGCGGTTCGGGG
TCTCGGTGGTACGGGAGGGTTCCTCGTTCGAGGTGGAGGGACGGCCTCGCTCACCCGGGAAGTTGCGGGTCGAGAACGAT
TGGAGTTCCGCGGGGTACTTCGTCGCGCTCGGCGCGATAGGTGGTGAGATGAGAATCGAGGGGGTGGACCTCGACTCCTC
GCACCCGGATCGCAGGATCGTGGAGATCACGCGCGAGATGGGGGCCGAGGTCCGTAGGATCGACGGCGGTATCGTCGTGC
GTTCGACGGGTCGACTGGAGGGGGTCGAGGTGGACCTCTCCGACTCGCCCGACCTGGTCCCGACCGTGGCGGCGATGGCG
TGCTTCGCGGAGGGGGTCACGAGGATCGAGAACGTGGGTCACCTGCGGTACAAAGAGGTGGACAGGCTTCGGGCGCTCGC
GGCGGAGCTACCCAAGTTCGGGGTGGAGGTAAGGGAGGGGAAGGATTGGCTGGAGATCGTGGGTGGAGAGCCGGTCGGGG
CACGGGTCGATTCCCGGGGTGATCATCGGATGGCGATGGCGCTGGCGGTGGTGGGAGCCTTCGCACGGGGTAAAACGGTC
GTCGAGCGCGCGGACGCGGTGTCCATCTCCTACCCGAGGTTCTGGGAGGATCTGGCGTCGGTCGGGGTGCCGGTCCACTC
AGTGTAG

Upstream 100 bases:

>100_bases
ACGACGACCGACTCCTCGGACATGGTCATCACCCGATCGTGCACCGCTCCGTCCGTTTTAAATCGGTTACCATCCCGAGG
GGTCGATCGGGGAGATCCAC

Downstream 100 bases:

>100_bases
GTTCGAGTGTCGGATCTCGAAGGCGCACGCGTTCACCAGCTCGCCGGCGCGGACCGCGCACTTGGCCTCCCTCACCTTAT
GCGGCTCTCGGCCGAAAGCC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRMLGAEVDVDGEERLEATVSGFG
DSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDSLRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPL
RERVAVYGDVSSQFVSALLFLGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVEND
WSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLEGVEVDLSDSPDLVPTVAAMA
CFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREGKDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTV
VERADAVSISYPRFWEDLASVGVPVHSV

Sequences:

>Translated_428_residues
MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRMLGAEVDVDGEERLEATVSGFG
DSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDSLRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPL
RERVAVYGDVSSQFVSALLFLGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVEND
WSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLEGVEVDLSDSPDLVPTVAAMA
CFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREGKDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTV
VERADAVSISYPRFWEDLASVGVPVHSV
>Mature_428_residues
MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRMLGAEVDVDGEERLEATVSGFG
DSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDSLRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPL
RERVAVYGDVSSQFVSALLFLGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVEND
WSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLEGVEVDLSDSPDLVPTVAAMA
CFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREGKDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTV
VERADAVSISYPRFWEDLASVGVPVHSV

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=430, Percent_Identity=35.5813953488372, Blast_Score=211, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6320332, Length=458, Percent_Identity=29.2576419213974, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_METKA (Q8TXN4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613913.1
- ProteinModelPortal:   Q8TXN4
- SMR:   Q8TXN4
- GeneID:   1476729
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0628
- NMPDR:   fig|190192.1.peg.626
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- BioCyc:   MKAN190192:MK0628-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45828; Mature: 45828

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRML
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHH
GAEVDVDGEERLEATVSGFGDSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDS
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCEEHHHCCCEEEECCHH
LRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPLRERVAVYGDVSSQFVSALLF
HHCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHH
LGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVEND
HHCCCCCEEEEEHHHHCCCCEEEHHHHHHHHCCCEEEECCCCEEECCCCCCCCEEEEECC
WSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLE
CCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHEECCCCEEEEECCCEE
GVEVDLSDSPDLVPTVAAMACFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECC
KDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTVVERADAVSISYPRFWEDLAS
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEECCHHHHHHHHH
VGVPVHSV
CCCCCCCC
>Mature Secondary Structure
MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRML
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHH
GAEVDVDGEERLEATVSGFGDSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDS
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCEEHHHCCCEEEECCHH
LRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPLRERVAVYGDVSSQFVSALLF
HHCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHH
LGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVEND
HHCCCCCEEEEEHHHHCCCCEEEHHHHHHHHCCCEEEECCCCEEECCCCCCCCEEEEECC
WSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLE
CCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHEECCCCEEEEECCCEE
GVEVDLSDSPDLVPTVAAMACFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECC
KDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTVVERADAVSISYPRFWEDLAS
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEECCHHHHHHHHH
VGVPVHSV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014