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The map label for this gene is GuaA_2

Identifier: 20093989

GI number: 20093989

Start: 513689

End: 514252

Strand: Direct

Name: GuaA_2

Synonym: MK0551

Alternate gene names: 20093989

Gene position: 513689-514252 (Clockwise)

Preceding gene: 20093988

Following gene: 20093990

Centisome position: 30.31

GC content: 58.33

Gene sequence:

>564_bases
TTGATCCTCATAATCGACAACCACGGCCAATACGTGCACCTGATCCGCAAGAACTTCGACTACATGGGTGTCCCCGCCGA
GATCATCCCCAACACCACCGACCCCGAAGACGTCCGCGAGCGTGCGAGCGGTGTGGTGATCAGCGGAGGACCATCCCGTG
AGCGGGCGGGTAACTCCCGAGAGATCATCGAGGAACTGACGGGAGAGGTACCTATACTGGGAATCTGCTTGGGTCACCAG
CTCATGGCGGAGGTTTTCGGAGGAAAGGTGGACTGGGCCGCGGGCCGTGAAGAATACGCCAGGACCGAGGTGGAGATACT
GGACCATGAAGGCATCTTCGAAGGCTTACCGGATAAGATCGTGGCTTGGGCCTCGCACCGGGACGAGGTGAAGGAAGTCC
CAGATGAGTTCGTCGTCACGGCCCGCTCCGACAGATGCGAGGTAGAGGCGATGCGTCACGAGGAGTTACCATTATACGGG
GTCCAGTTCCACCCCGAGCTGAAGTTCACCGAGTACGGGCCGGATATACTGAAGAACTTCGCGAAACTATGTGGCGAACT
GTAA

Upstream 100 bases:

>100_bases
GAAAAAGAGGACCTTAGAGCGCTCTCGGAGGACGTCGCCCGTATTACCGGCGTGAAGATGGCCGGGTCCGACGTCGAGCT
GGCCTAGGGGCCTCTCCCCG

Downstream 100 bases:

>100_bases
AGATGGGGGGTAGCACGACGAAGTTCGGGTTCTTCACCCTCGTAGGTTACACGGCGTTACTCCTGTCCTTAGGAGCGATC
GTCGCGAGACTGTCCGAAGA

Product: GMP synthase subunit A

Products: NA

Alternate protein names: Glutamine amidotransferase

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSREIIEELTGEVPILGICLGHQ
LMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKIVAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYG
VQFHPELKFTEYGPDILKNFAKLCGEL

Sequences:

>Translated_187_residues
MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSREIIEELTGEVPILGICLGHQ
LMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKIVAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYG
VQFHPELKFTEYGPDILKNFAKLCGEL
>Mature_187_residues
MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSREIIEELTGEVPILGICLGHQ
LMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKIVAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYG
VQFHPELKFTEYGPDILKNFAKLCGEL

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=181, Percent_Identity=35.9116022099448, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1788854, Length=191, Percent_Identity=32.4607329842932, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=36.8983957219251, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787517, Length=186, Percent_Identity=28.494623655914, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901714, Length=184, Percent_Identity=34.2391304347826, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992717, Length=184, Percent_Identity=34.2391304347826, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992710, Length=184, Percent_Identity=34.2391304347826, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323873, Length=185, Percent_Identity=35.6756756756757, Blast_Score=108, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322638, Length=190, Percent_Identity=31.0526315789474, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281365319, Length=181, Percent_Identity=32.5966850828729, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAAA_METKA (Q8TXV8)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613836.1
- ProteinModelPortal:   Q8TXV8
- SMR:   Q8TXV8
- GeneID:   1476652
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0551
- NMPDR:   fig|190192.1.peg.549
- HOGENOM:   HBG292341
- OMA:   GQYVHRI
- ProtClustDB:   PRK00758
- BioCyc:   MKAN190192:MK0551-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_01510
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR004739
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase

EC number: =6.3.5.2

Molecular weight: Translated: 21042; Mature: 21042

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 76-76 ACT_SITE 164-164 ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSR
CEEEEECCCCEEEEEHHCCCCCCCCHHHCCCCCCHHHHHHHHCCEEEECCCCHHHCCCHH
EIIEELTGEVPILGICLGHQLMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKI
HHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCHHHCCCHHH
VAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYGVQFHPELKFTEYGPDILKNF
HHHHCCHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
AKLCGEL
HHHHCCC
>Mature Secondary Structure
MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSR
CEEEEECCCCEEEEEHHCCCCCCCCHHHCCCCCCHHHHHHHHCCEEEECCCCHHHCCCHH
EIIEELTGEVPILGICLGHQLMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKI
HHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCHHHCCCHHH
VAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYGVQFHPELKFTEYGPDILKNF
HHHHCCHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
AKLCGEL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014