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The map label for this gene is TrpF

Identifier: 20093946

GI number: 20093946

Start: 469072

End: 469722

Strand: Direct

Name: TrpF

Synonym: MK0508

Alternate gene names: 20093946

Gene position: 469072-469722 (Clockwise)

Preceding gene: 20093945

Following gene: 20093948

Centisome position: 27.67

GC content: 61.75

Gene sequence:

>651_bases
GTGGTCAGGGTGAAGATTTGCGGCATAACCAGGCCCGAGGACGCCGCGACCGCCGACGAGGCGGGAACCGACGCCGTCGG
TTGCGTGGTAGAAGTTCCCGTCTCCACACCGCGAAAGGTGTCCGCGGAGCACGCGAACGAGGTATTCTCCGTAGTTTCAC
CGTTCGTGTCGAGGGTCGCCGTGCTGATGGACAACCTAGAGCCTATCGACCGGCTGGAAGAAGCCACCGCCGTGCAGCTT
CACGGGACGGAGGATCCGGAAACCTGTGAAGAGCTCAGCGAGCTCGGGTTAGACGTCATCAAGACGTTCTGGGTGGATCA
GCGAGGGTCCGTATGGTTGGGAGAGGAACTGATAGGGGACGAGGTGCTGGCGGAGTACTGTGAGATCGTGGATGCCGTGC
TCCTGGACACCAAATCCGCGGAAGGGGGAGGGTCCGGCGAGCGCCACGACTGGGACGCGTCGGCGCGATTGGTCAGGCGA
CTGGACGTACCCGTGATCTTAGCGGGCGGTCTGAACCCGGAGAACGTTCGAGAGGCCGTTGAGAAGGTCCGTCCGTACGC
CGTCGACACATCTTCGGGCGTAGAGAAGGAGCCGGGAATCAAGGATCCGGAAGCTATAGCGGAGTTCGTAAGGGCTACCA
AGTCGGTTTAA

Upstream 100 bases:

>100_bases
GGCTGGAGTGGAAGAAGCTAGAACCCGTACCGCTGAGGGACGATGGTGGTAAGAAGCAGTGCACGCTCACGGAGTTCCTG
GCGGGGGAGGGAGACTGACC

Downstream 100 bases:

>100_bases
TCGGGACACTGGCGCGTCTCATCCACACGAGCCAGCATACCAGCGTTCCTACCACGGCGGCCATAACCAGGTACCTGAGA
ATCGGTGGCACGGGTGAACC

Product: phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVAVLMDNLEPIDRLEEATAVQL
HGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGDEVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRR
LDVPVILAGGLNPENVREAVEKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV

Sequences:

>Translated_216_residues
MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVAVLMDNLEPIDRLEEATAVQL
HGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGDEVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRR
LDVPVILAGGLNPENVREAVEKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV
>Mature_216_residues
MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVAVLMDNLEPIDRLEEATAVQL
HGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGDEVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRR
LDVPVILAGGLNPENVREAVEKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=213, Percent_Identity=31.4553990610329, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320210, Length=226, Percent_Identity=29.646017699115, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_METKA (Q8TXZ9)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613793.1
- ProteinModelPortal:   Q8TXZ9
- SMR:   Q8TXZ9
- GeneID:   1476609
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0508
- NMPDR:   fig|190192.1.peg.506
- HOGENOM:   HBG554803
- OMA:   DSWNKET
- BioCyc:   MKAN190192:MK0508-MONOMER
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_A
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 23294; Mature: 23294

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVA
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLMDNLEPIDRLEEATAVQLHGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGD
HHHHCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHH
EVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRRLDVPVILAGGLNPENVREAV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHH
EKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV
HHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVA
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLMDNLEPIDRLEEATAVQLHGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGD
HHHHCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHH
EVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRRLDVPVILAGGLNPENVREAV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHH
EKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV
HHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014